Decreased motility of human spermatozoa presenting phosphatidylserine membrane translocation-cells selection with the swim-up technique

Kotwicka, Małgorzata; Jendraszak, Magdalena; Skibinska, Izabela; Jȩdrzejczak, Piotr; Pawełczyk, Leszek

doi:10.1007/s13577-011-0024-1

Cited by 8 publications

(7 citation statements)

References 34 publications

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“…In this investigation, PS(40:2) was found significantly decreased in infertile patients ( Figure 5 ). PS are known to be involved in apoptotic cell events through the process of PS membrane translocation, which is recognized as an important biological event in the cell ( Kotwicka et al, 2013 ). It was suggested that PS membrane translocation could be responsible for the changes in the spermatozoa cell membrane which happen during capacitation and acrosome reaction or during the elimination of pathological spermatozoa ( Kotwicka et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Seminal plasma untargeted metabolomic and lipidomic profiling for the identification of a novel panel of biomarkers and therapeutic targets related to male infertility

Correnti,

Preianò,

Fregola

et al. 2023

Front. Pharmacol.

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Male infertility occurs approximately in about 50% of all infertility cases and represents a serious concern worldwide. Traditional semen analysis alone is insufficient to diagnose male infertility. Over the past two decades, advances in omics technologies have led to the widespread application of metabolomics profiling as a valuable diagnostic tool for various diseases and disorders. Seminal plasma represents a rich and easily accessible source of metabolites surrounding spermatozoa, a milieu that provides several indispensable nutrients to sustain sperm motility and fertilization. Changes of metabolic profiles in seminal plasma reflect male reproductive tract disorders. Here, we performed seminal plasma metabolomics and lipidomics profiling to identify a new pattern of biomarkers of male infertility. Seminal plasma samples from unfertile subjects (n = 31) and fertile controls (n = 19) were analyzed using an untargeted metabolomics/lipidomics integrated approach, based on Ultra-High-Pressure Liquid Chromatography–tandem Mass Spectrometry. Partial Least Squares-Discriminant Analysis showed a distinct separation between healthy fertile men and infertile subjects. Among the 15 selected candidate biomarkers based on Variable Importance in Projection scores, phosphatidylethanolamine (PE) (18:1; 18:1) resulted with the highest score. In total, 40 molecular species showed statistically significant variations between fertile and infertile men. Heat-map and volcano plot analysis indicated that acylcarnitines, phosphatidylserine (PS) (40:2) and lactate were decreased, while PE (18:1; 18:1), Phosphatidic acid (PA) (O-19:2; 18:1), Lysophosphatidylethanolamine (LPE) (O-16:1) and Phosphatidylcholine (PC) (O-16:2; 18:1)-CH3 were increased in the infertile group. The present study is the first one to analyze the metabolomics/lipidomics dysregulation in seminal plasma between fertile and infertile individuals regardless of sub-infertility condition. Association of several metabolites/lipids dysregulation with male infertility reinforced data of previous studies performed with different approaches. In particular, we confirmed significantly decreased levels of PS and carnitines in infertile patients as well as the positive correlation with sperm motility and morphology. If validated on a larger prospective cohort, the metabolite biomarkers of infertility in seminal plasma we identified in the present study might inform novel strategies for diagnosis and interventions to overcome male infertility.

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Section: Discussionmentioning

confidence: 99%

Seminal plasma untargeted metabolomic and lipidomic profiling for the identification of a novel panel of biomarkers and therapeutic targets related to male infertility

Correnti,

Preianò,

Fregola

et al. 2023

Front. Pharmacol.

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“…OEC would also be able to discriminate between annexin negative spermatozoa, with their morphologically superior quality, and annexin positive sperm. 58 Moreover, PS translocation is associated with a decrease in the adenosine triphosphate (ATP) level of spermatozoa 59 and with a lower mitochondrial potential, 60 and so the OECs do not bind these spermatozoa because they have insufficient energy reserves to remain in the sperm reservoir and perform fertilization. Another explanation is that phospholipids (including PS) are incorporated into the sperm membrane by OEC 61 as a selection mechanism.…”

Section: Discussionmentioning

confidence: 99%

Oviductal epithelial cells selected boar sperm according to their functional characteristics

et al. 2017

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The interaction of oviductal epithelial cells (OECs) with the spermatozoa has beneficial effects on the sperm functions. The aim of this study is to evaluate the in vitro fertilizing capacity of incubating spermatozoa previously selected by density gradient in OEC and determinate some sperm characteristics that could explain the results obtained. In this study, we assessed in vitro fertilization (IVF), tyrosine phosphorylation, phosphatidylserine translocation, nuclear DNA fragmentation, and chromatin decondensation. Three experimental sperm groups, previously selected by Percoll gradient, were established according to the origin of the sperm used for IVF: (i) W30 group: spermatozoa were incubated with oocytes in the absence of OEC; (ii) NB group: after sperm incubation in OEC, the unbound spermatozoa were incubated with oocytes, in the absence of OEC; and (iii) B group: after sperm incubation with OEC, the bound spermatozoa were incubated with oocytes in the OEC plates. The results showed that sperm from the NB group led to a lower IVF yield, accompanied by low penetration rates (NB: 19.6%, B: 94.9%, and W30: 62.9%; P < 0.001) and problems of nuclear decondensation. Moreover, higher levels of tyrosine phosphorylation were observed in the NB group compared with the W30 and B groups (NB: 58.7%, B: 2.5%, and W30: 4.5%; P < 0.01). A similar trend was observed in phosphatidylserine translocation (NB: 93.7%, B: 5.7%, and W30: 44.2%; P < 0.01). These results demonstrate that the OEC exerts a rigorous degree of sperm selection, even within an already highly selected population of spermatozoa, and can capture the best functional spermatozoa for fertilization.

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“…In such a context, our observation about positive correlation between sperm apoptotic markers and the fertilization rate of conventional IVF appears well grounded. The midpiece location of the PST and activation of caspase-3 suggest mitochondrial dysfunction, both by the location itself and by decreased sperm motility [ 20 ] resulting in impaired fertility potential. Nevertheless, the active caspase-3 presence does not represent a decisive indicator for cell death as the time of spermatozoa vital time was showed not to increase after incubation with caspases inhibitors [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…Higher PST was found in men with lower sperm quality than sperm donors [ 17 – 19 ]. The presence of PST is related to lower vitality and motility of the sperm cells [ 20 ]. In contrast, Ricci et al [ 21 ] did not find correlation between PST and sperm parameters [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Sperm midpiece apoptotic markers: impact on fertilizing potential in in vitro fertilization and intracytoplasmic sperm injection

et al. 2016

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The aim of this study was to investigate the relationship between apoptotic markers present in human spermatozoa, namely phosphatidylserine translocation (PST) from the inner to the outer layer of the cytomembrane and the active form of caspase-3 (c3) versus the fertilizing potential of male gametes in conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) models. A total of 116 male patients treated with their partners for infertility underwent basic semen analysis and an assessment of the presence of PST and the active c3 in sperm using flow cytometry. Forty patients underwent IVF, group A, while 76 patients underwent ICSI, group B. The fertilizing potential of the gametes was measured as the percentage of oocytes with pronuclei present after either procedure. PST and active c3 were identified in vital gametes, mainly in the midpiece area. Concentration, motility, morphology, and viability of spermatozoa strongly negatively correlated with both markers. In group A, a negative correlation between both markers and the success rate of conventional IVF was observed (r = −0.4, p = 0.04 for PST; r = −0.4, p = 0.02 for active c3, respectively). In group B, the success rate of ICSI did not correlate with either marker (r = −0.2, p = 0.85 for PST and r = 0.1, p = 0.51 for active c3). The two apoptotic markers localized in the sperm midpiece area may affect their function not only by decreasing basic andrologic parameters but also by reducing the probability of conception. Therefore, analysis of PST and active c3 in the sperm of patients undergoing infertility treatment should be recommended.

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Decreased motility of human spermatozoa presenting phosphatidylserine membrane translocation-cells selection with the swim-up technique

Cited by 8 publications

References 34 publications

Seminal plasma untargeted metabolomic and lipidomic profiling for the identification of a novel panel of biomarkers and therapeutic targets related to male infertility

Seminal plasma untargeted metabolomic and lipidomic profiling for the identification of a novel panel of biomarkers and therapeutic targets related to male infertility

Oviductal epithelial cells selected boar sperm according to their functional characteristics

Sperm midpiece apoptotic markers: impact on fertilizing potential in in vitro fertilization and intracytoplasmic sperm injection

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