Decreased microRNA-155 Expression in Ocular Behcet's Disease but Not in Vogt Koyanagi Harada Syndrome

A predominant Th17 population is a marker of allergic rhinitis (AR). The aryl hydrocarbon receptor (AhR) exhibits strong immunomodulation potential via regulation of the differentiation of T lymphocytes and dendritic cells (DCs) after activation by its ligand, such as 2-(1 0 H-indole-3 0 -carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE). The aim of this study was to analyze the effect of AhR on Th17 differentiation by investigating the action of ITE on DCs and CD4 þ T cells from patients with AR. In all, 26 AR patients and 12 healthy controls were included in this study. The expression of interleukin (IL)-1b, IL-6, IL-10, and IL-17 in the culture supernatant and the presence of Th17 cells in CD4þ T cells and DC-CD4 þ T-cell co-culture system were measured before and after treatment with ITE. We show that ITE significantly induced cell secretion of IL-10 and inhibited IL-1b and IL-6 production in DCs, and promoted IL-10 production and suppressed IL-17 expression in CD4 þ T cells in vitro. It also suppressed the expansion of Th17 cells in vitro. Our work demonstrates that ITE acts on DCs and CD4 þ T cells to inhibit the Th17 response that suppresses AR; the AhR-DC-Th17 axis may be an important pathway in the treatment of AR. ITE, a nontoxic AhR ligand, attenuated the Th17 response; thus, it appears to be a promising therapeutic candidate for suppressing the inflammatory responses associated with AR.

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“…17 The supernatants were harvested for IL-17 ELISA. The cells were collected for flow cytometry analysis.…”

Section: Dc-cd4mentioning

confidence: 99%

An aryl hydrocarbon receptor ligand acts on dendritic cells and T cells to suppress the Th17 response in allergic rhinitis patients

Wei

Kang

et al. 2014

Laboratory Investigation

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“…Kaneko et al [49] reported that no microRNA was significantly overexpressed in sympathetic Characterization of microRNA expression profiling in peripheral… ophthalmia, a bilateral diffuse granulomatous uveitis of both eyes following trauma to one eye, whereas 27 miRNAs were markedly downregulated, suggesting that downregulated miRNAs may play a crucial role in the pathogenesis of sympathetic ophthalmia. Zhou et al [50] also reported that only miR-155 expression was significantly decreased in peripheral blood mononuclear cells and dendritic cells from Behcet's disease patients with active uveitis. Taken together, our findings substantiate that downregulated miRNAs may play a crucial role in the pathogenesis of uveitis either in experimental animals or in human being.…”

Section: Discussionmentioning

confidence: 96%

Characterization of microRNA expression profiling in peripheral blood lymphocytes in rats with experimental autoimmune uveitis

Guo

Liu

et al. 2015

Inflamm. Res.

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“…VKH is an autoimmune disorder with meningismus, cerebrospinal fluid pleocytosis, auditory findings and integumentary findings. In a study to investigate the role of miRNAs in the pathogenesis of uveitis, miR-155, an immunologically relevant miRNA, was found to be expressed less in peripheral-blood mononuclear cells and dendritic cells of BD patients than of healthy controls [69]. No such difference was detected between VKH patients and healthy controls.…”

Section: Mirnas and Eye Disordersmentioning

confidence: 99%

“…In addition to TAB2, IL-6 and IL-1β were found to be the targets for miR-155. In contrast to the decreased expression of miR-155, TAB2 expression was found to be increased in BD patients with active uveitis when compared to healthy controls [69]. miR-155 expression is downregulated in the pathogenesis of BD but not in VKH.…”

Section: Mirnas and Eye Disordersmentioning

confidence: 99%

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Micro-RNAs and Their Roles in Eye Disorders

Raghunath¹,

Perumal²

2015

Ophthalmic Res

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Micro-RNAs (miRNAs) are members of the family of noncoding RNA molecules that regulate gene expression by translational repression and mRNA degradation. Initial identification of miRNAs revealed them only as developmental regulators; later, their radiated roles in various cellular processes have been established. They regulate several pathways, including developmental timing, hematopoiesis, organogenesis, apoptosis, cell differentiation and proliferation. Their roles in eye disorders are being explored by biologists around the world. Eye physiology requires the perfect orchestration of all the regulatory networks; any defect in any of the networks leads to eye disorders. The dysregulation of miRNA expression has been reported in many eye disorders, which paves the way for new therapeutics. This review summarizes the biogenesis of miRNAs and their role in eye disorders. miRNA studies also have implications for the understanding of various complex metabolic pathways leading to disorders of the eye. The ultimate understanding leads to potential opportunities in evaluating miRNAs as molecular biomarkers, prognostic tools, diagnostic tools and therapeutic agents for eye disorders.

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Decreased microRNA-155 Expression in Ocular Behcet's Disease but Not in Vogt Koyanagi Harada Syndrome

Abstract: The present results suggest that miR-155 expression is decreased in active BD but not in VKH patients. Downregulated miR-155 may be involved in BD pathogenesis by targeting TAB2.

Cited by 75 publications

References 37 publications

An aryl hydrocarbon receptor ligand acts on dendritic cells and T cells to suppress the Th17 response in allergic rhinitis patients

An aryl hydrocarbon receptor ligand acts on dendritic cells and T cells to suppress the Th17 response in allergic rhinitis patients

Characterization of microRNA expression profiling in peripheral blood lymphocytes in rats with experimental autoimmune uveitis

Micro-RNAs and Their Roles in Eye Disorders

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