Decreased levels of heat shock proteins in gut epithelial cells after exposure to plant lectins

Ovelgönne, J. H.; Koninkx, J. F. J. G.; Pusztai, Á.; Bardócz, Susan; Kok, W; Ewen, S. W. B.; Hendriks, Henno; Dijk, J. E. van

doi:10.1136/gut.46.5.680

Cited by 36 publications

(33 citation statements)

References 52 publications

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“…This effect was attributed to an activation of the cellular defence system manifested by overexpression of Hsp70 (Oxman et al, 2000). We previously demonstrated the ability of enterocyte-like Caco-2 cells to produce the protective Hsp70 under various conditions (Ovelgönne et al, 2000;Malago et al, 2003). In this study, we report that L. casei subsp casei 2756, L. curvatus 2775, and L. plantarum 2142 and their SCS are capable of inducing Hsp70 expression in the Caco-2 cells (Figs.…”

Section: Discussionmentioning

confidence: 65%

“…This response is specific to the bacteria and their SCS since the MRS broth or variations in pH could not induce the Hsp70 expression. Because the production of Hsp70 is a protective response (Malago et al, 2002;Ovelgönne et al, 2000), we suggest that one mechanism for the beneficial attributes of the non-starter lactobacilli is the ability to induce the expression of Hsp70 by the bacteria and their antimicrobial products. The induced Hsp70 could function in the stabilisation of the cytoskeleton of intestinal epithelial cells after being distorted during adhesion and invasion by Salmonella (Jepson et al, 1995(Jepson et al, , 1996.…”

Section: Discussionmentioning

confidence: 99%

“…Secondly, we investigated whether these bacteria and their microbial products induce the expression of Hsp70 that can account for their beneficial effects to the gut. We used enterocyte-like Caco-2 cells, an in vitro model of the human intestinal epithelium that differentiate in culture and acquire characteristics both structurally and functionally of either crypt cells (5-day-old Caco-2 cells) or villus cells (19-day-old Caco-2 cells) of the small intestine (Koninkx et al, 1992;Koninkx, 1995;Ovelgönne et al, 2000;Malago et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of Salmonella-induced IL-8 synthesis and expression of Hsp70 in enterocyte-like Caco-2 cells after exposure to non-starter lactobacilli

Németh

Fajdiga

Malago

et al. 2006

International Journal of Food Microbiology

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Oral administration of lactobacilli as probiotics is gaining importance in the treatment of intestinal inflammations. We investigated the effect of non-starter lactobacilli Lactobacillus casei subsp casei 2756, Lactobacillus curvatus 2775, and Lactobacillus plantarum 2142 as well as their spent culture supernatants (SCS) on Salmonella enteritidis 857 growth, interleukin (IL)-8 and heat shock protein 70 (Hsp70) synthesis in undifferentiated crypt-like and differentiated villus-like Caco-2 cells. The cells were infected with graded numbers of non-starter lactobacilli or S. enteritidis 857 for 1 h and allowed to recover for 24 h or exposed to 200 bacteria/cell for 1 h and allowed to recover for different periods of time. In another experiment S. enteritidis 857 was first pre-treated with SCS-lactobacilli for 1 h before infecting the cells. The levels of IL-8 and Hsp70 were assessed using sandwich ELISA and immunostaining of Western blots, respectively. The effect of SCS-lactobacilli on S. enteritidis 857 growth was evaluated by agar plate diffusion test.The non-starter lactobacilli induced a significant increase in the levels of both IL-8 and Hsp70. However, compared with the S. enteritidis 857 induced IL-8 synthesis, the levels of IL-8 induced by the lactobacilli at any equivalent bacterial number were far lower. After exposure of Caco-2 cells to S. enteritidis 857 pre-treated with SCS-lactobacilli, it appeared that their SCS inhibited the S. enteritidis 857 growth and IL-8 synthesis and in addition induced the expression of Hsp70. The differences in response of crypt-and villus-like Caco-2 cells are merely a reflection of their differentiation status.Our data suggest that the beneficial effect of non-starter lactobacilli to the intestinal inflammations might be associated with a decrease of the IL-8 levels. This effect could be mediated, at least in part, by the bacteria themselves or via a secreted antimicrobial product(s) either directly against the pathogens or indirectly through the synthesis of Hsp70.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibition of Salmonella-induced IL-8 synthesis and expression of Hsp70 in enterocyte-like Caco-2 cells after exposure to non-starter lactobacilli

Németh

Fajdiga

Malago

et al. 2006

International Journal of Food Microbiology

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“…They have been shown to decrease soluble iHSP70 in both Caco2 cells and intestinal tissues in rats. 148 These data were interpreted as evidence of cytoplasmic iHSP70 reduction due to iHSP70 rescue migration to cell membranes. Exposure to gliadin, a wheat protein toxic to celiac patients, was recently demonstrated to induce iHSP70 redistribution to the cytoskeleton following junctional protein (ezrin and E-cadherin) disruption in Caco2 cells.…”

Section: Proteins and Peptidesmentioning

confidence: 99%

Gut epithelial inducible heat-shock proteins and their modulation by diet and the microbiota

Arnal

Lallès

2016

Nutr Rev

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“…Changes in growth curve characteristics, cell metabolism, cell differentiation and TEER of enterocyte-like Caco-2 cells after exposure to the experimental diets Enterocyte-like Caco-2 cells routinely grown in supplemented Dulbecco's modified Eagle's medium (DMEM) were seeded at 40 000 cells/cm 2 (Ovelgö nne et al, 2000;Malago et al, 2003;Malago et al, 2005). In the case of the TEER experiments, using Transwell polycarbonate filter inserts (Costar Europe, Badhoevedorp, The Netherlands), 60 000 cells/cm 2 were seeded.…”

Section: Designmentioning

confidence: 99%

Effects of prefermented cereals or the end products of fermentation on growth and metabolism of enterocyte-like Caco-2 cells and on intestinal health of restrictedly fed weanling pigs

Bruininx

Koninkx

Binnendijk

et al. 2010

Animal

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To unravel the underlying mechanisms that explain the positive effects of prefermented cereals on in vivo gastrointestinal (GI) architecture and function, an in vitro experiment using a human small intestinal epithelial cell model (Caco-2) was performed. A range of dilutions (0% to 10%) of the supernatants of three liquid experimental diets, as well as Na-lactate were used in an in vitro experiment to assess their effect on cellular growth, metabolism, differentiation and mucosal integrity using Caco-2. The experimental diets contained, in addition to a protein rich basal diet (60%), (1) a liquid control diet (C) containing 40% of a mixture of barley and wheat (ratio 3 : 1) or (2) a liquid diet (F) containing 40% prefermented barley and wheat or (3) C with the addition of the fermentation end-products (organic acids and ethanol) in concentrations similar to those in the fermented diet (FP). For F, the mixture of barley and wheat was fermented at 358C for 48 h. Parallel to the in vitro experiment, 18 groups of eight weanling pigs were assigned to one of the experimental diets during a 14-day in vivo experiment. Each group was fed restrictively. The results of the in vitro experiment showed that the lowest dose of both F-and FP-supernatants had no clear effects on the cell proliferation, but incubation with 5% and 10% of the F-and FP-supernatants decreased the cell numbers at day 19. DNA, RNA, protein and glycoprotein synthesis in differentiated Caco-2 cells were stimulated by incubation with the lower concentrations (0.5% to 2.5%) of F-and FP-supernatants whereas the higher concentrations (5% and 10%) had no effect. Both the F-and FP-supernatants decreased the specific sucrase-isomaltase activity in a dose-dependent manner, but the effects on the specific aminopeptidase activities were less clear. Mucosal integrity initially decreased after incubation with the highest F-and FP-supernatants and started to recover between 24 and 48 h. The results of the in vivo experiment showed no dietary effects ( P . 0.1) on GI morphology and brush-border enzyme activities at day 5 or at day 14. Time related changes in GI characteristics followed a normal pattern. In conclusion, the supernatants of diets containing either prefermented cereals or their fermentation end-products clearly modulate cellular growth, metabolism, differentiation and mucosal integrity in an in vitro model, although these effects were not observed in the in vivo characteristics measured in weanling pigs.Keywords: Caco-cell, fermented cereals, intestine, pigs ImplicationsThe described in vitro experiment showed that fermentation end products within the range of realized intake in the in vivo study, affect metabolism of intestinal cells. Measurements point at an initial damaging effect followed by repair.The described in vivo experiment did not show effects on intestinal physiology on day 5 and 14 after weaning. It seems that the provision of fermented cereals has several effects of which the net outcome is not always beneficial for the animal. The in vitro...

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Decreased levels of heat shock proteins in gut epithelial cells after exposure to plant lectins

Abstract: (Gut 2000;46:679-687)

Cited by 36 publications

References 52 publications

Inhibition of Salmonella-induced IL-8 synthesis and expression of Hsp70 in enterocyte-like Caco-2 cells after exposure to non-starter lactobacilli

Inhibition of Salmonella-induced IL-8 synthesis and expression of Hsp70 in enterocyte-like Caco-2 cells after exposure to non-starter lactobacilli

Gut epithelial inducible heat-shock proteins and their modulation by diet and the microbiota

Effects of prefermented cereals or the end products of fermentation on growth and metabolism of enterocyte-like Caco-2 cells and on intestinal health of restrictedly fed weanling pigs

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