Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients

Abstract

BackgroundT-cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. Based on our previous finding, to further characterize the immune status, T cell proliferative history was analyzed in CD4+ and CD8+ T cells from chronic myeloid leukemia (CML) patients.MethodsQuantitative analysis of δRec-ψJα signal joint T cell receptor excision circles (sjTRECs) was performed in PBMCs, CD3+, CD4+ and CD8+T cells by real-time PCR, and the analysis of 23 TRBV-D1 sjTRECs was performed by semi-nested PCR. Forty eight CML cases in chronic phase (CML-CP) were selected for this study and 17 healthy individuals served as controls.ResultsThe levels of δRec-ψJα sjTRECs in PBMCs, CD3+, CD4+, and CD8+ T cells were significantly decreased in CML patients, compared with control groups. Moreover, the numbers of detectable TRBV subfamily sjTRECs, as well as the frequency of particular TRBV-BD1 sjTRECs in patients with CML were significantly lower than those from healthy individuals.ConclusionsWe observed decreased levels of recent thymic emigrants in CD4+ and CD8+ T cells that may underlay the persistent immunodeficiency in CML patients.

DNA demethylating agent decitabine broadens the peripheral T cell receptor repertoire

“…For TRECs analysis, a semi-nested PCR was performed as previously reported [38]. For the first PCR, 200 ng of genomic DNA from PBMC was amplified in a 10 μl reaction system containing external forward and reverse primers.…”

Eight-color immunophenotyping of T-, B-, and NK-cell subpopulations for characterization of chronic immunodeficiencies

“…As mentioned above, the numbers of RTEs leaving the thymus decreases with age, resulting in fewer CD311 T-cells. The determination of RTEs in correlation to age-matched reference values is of clinical relevance, as it helps to estimate the status of disease progression, such as in chronic myeloid leukemia or autoimmune thyroid disease (53)(54)(55). RTE measurement has also been shown to be helpful for the detection of PID such as in DiGeorge syndrome, which is accompanied by reduced production of RTEs already at early life (7).…”

Upregulated TCRζ Enhances Interleukin-2 Production in T-Cells from Patients with CML

“…Such immune dysfunction, which has been demonstrated in many patients with leukemia, may be due to a disorder in the thymic output function, and the abnormal expression of the T cell receptor (TCR) repertoire and may, in part, be due to abnormal TCR signal transduction, possibly through altered expression of the CD3 genes (Rezvany et al, 1999;Chen et al, 2000;Torelli et al, 2003;Li, 2008;Chen et al, 2009;Li et al, 2010;Li et al, 2011b). In CML, decreased levels of recent thymic emigrants in both CD4 + and CD8 + T cells may underlie the persistent immunodeficiency found in patients.…”