Decreased intrinsic excitability of cerebellar Purkinje cells following optokinetic learning in mice

Kim, Yong Gyu; Kim, Sang Jeong

doi:10.1186/s13041-020-00678-2

Cited by 8 publications

(12 citation statements)

References 15 publications

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“…The final OKR was measured immediately after completion of the consolidation session. Data analysis for oculomotor behavior was performed as described in our prior study [ 21 ]. The gain was defined as the ratio of eye velocity to stimulus velocity.…”

Section: Methodsmentioning

confidence: 99%

Kdm3b haploinsufficiency impairs the consolidation of cerebellum-dependent motor memory in mice

et al. 2021

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Histone modifications are a key mechanism underlying the epigenetic regulation of gene expression, which is critically involved in the consolidation of multiple forms of memory. However, the roles of histone modifications in cerebellum-dependent motor learning and memory are not well understood. To test whether changes in histone methylation are involved in cerebellar learning, we used heterozygous Kdm3b knockout (Kdm3b+/−) mice, which show reduced lysine 9 on histone 3 (H3K9) demethylase activity. H3K9 di-methylation is significantly increased selectively in the granule cell layer of the cerebellum of Kdm3b+/− mice. In the cerebellum-dependent optokinetic response (OKR) learning, Kdm3b+/− mice show deficits in memory consolidation, whereas they are normal in basal oculomotor performance and OKR acquisition. In addition, RNA-seq analyses revealed that the expression levels of several plasticity-related genes were altered in the mutant cerebellum. Our study suggests that active regulation of histone methylation is critical for the consolidation of cerebellar motor memory.

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Section: Methodsmentioning

confidence: 99%

Kdm3b haploinsufficiency impairs the consolidation of cerebellum-dependent motor memory in mice

et al. 2021

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“…The black eye C57BL/6N male mice (body weight 20-25 g, 8 weeks old, OrientBio, Seoul, Korea) were employed in the experiment. Mice were equipped for behavioral tests as mentioned previously [8,10,12,13]. Briefly, under general anesthesia with isoflurane, a minimal incision in the scalp was made after application of lidocaine cream to reduce pain or discomfort.…”

Section: Preparation For Behavioral Tests and Okr Setupmentioning

confidence: 99%

“…Twelve cycles of elicited eye movements were chosen for average from sixty cycles, free of motion parallax and eye blinking, and the given stimulation and response were suited to sine curves. As previously described, we used a custum-made program in LabView to analyze the research data [8,10,12,13]. The standard deviation (SD) of the group averages is represented in the text.…”

Section: Eye Movement Recordings and Data Analysismentioning

confidence: 99%

“…The gain of OKR can be increased by prolonged oscillation of optokinetic drum in the absence of head movements, and this OKR gain adaption has been employed as a good experimental paradigm for researching cerebellum-dependent motor learning [1,[3][4][5][6][7][8][9]. The longterm depression at parallel fibers-Purkinje cell synapses and changes in intrinsic excitability of Purkinje cells in the flocculus have been suggested as mechanism for OKR motor learning [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Adaptation of Optokinetic Reflex by Training with Different Frequency and Amplitude

Pham¹,

Kim²,

Kim³

et al. 2022

J. Integr. Neurosci.

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Background: Although the occurrence of optokinetic reflex (OKR) adaptation after OKR training is well established, the dynamic properties of OKR adaptation has not been fully studied. This study aimed to examine the difference in the amount of OKR adaptation according to OKR training protocols which have different frequency or amplitude of drum oscillation. Methods: Using C57BL/6N male mice, we induced OKR adaptation by 3 different categories of learning paradigm as follows: (1) Optokinetic drum oscillation for 60 min with same amplitude and different frequency. (2) Optokinetic drum oscillation for 60 min with same frequency and different amplitude. ( 3) Training with serial combination of different frequency or amplitude. Results: The results show that the amount of OKR adaptation was greater after OKR training with lower frequency or amplitude than that with higher frequency or amplitude. Conclusions: This finding may suggest that the retinal slip signal with lower-velocity OKR stimulation serves as more precise instructive signal for learning, leading to induction of more efficient training effect. Another interesting finding was that the OKR gain increase tended to be greater after training composed of sequential combination of decreasing frequency or amplitude than that composed of sequential combination of increasing frequency or amplitude. Furthermore, the OKR training with high frequency or amplitude eliminated a part of learning effects which have already formed by previous training. We postulate that the stimulation during training with high frequency or amplitude may implement a disturbing instruction for OKR learning when it is conducted in mice with increased OKR gain after previous OKR training.

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“…Brain slices were prepared as described previously [11]. Brain slices (250 μm thick) were obtained using a vibratome (VT1200, Leica) after isoflurane anesthesia.…”

Section: Slice Preparationmentioning

confidence: 99%

Minhee Analysis Package: an integrated software package for detection and management of spontaneous synaptic events

2021

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To understand the information encoded in a connection between the neurons, postsynaptic current (PSC) has been widely measured as a primary index of synaptic strength in the field of neurophysiology. Although several automatic detection methods for PSCs have been proposed to simplify a workflow in the analysis, repetitive steps such as quantification and management of PSC data should be still performed with much effort. Here, we present Minhee Analysis Package, an integrated standalone software package that is capable of detecting, sorting, and quantifying PSC data. First, we developed a stepwise exploratory algorithm to detect PSC and validated our detection algorithm using the simulated and experimental data. We also described all the features and examples of the package so that users can use and follow them properly. In conclusion, our software package is expected to improve the convenience and efficiency of neurophysiologists to analyze PSC data by simplifying the workflow from detection to quantification. Minhee Analysis Package is freely available to download from http://www.github.com/parkgilbong/Minhee_Analysis_Pack.

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Decreased intrinsic excitability of cerebellar Purkinje cells following optokinetic learning in mice

Cited by 8 publications

References 15 publications

Kdm3b haploinsufficiency impairs the consolidation of cerebellum-dependent motor memory in mice

Kdm3b haploinsufficiency impairs the consolidation of cerebellum-dependent motor memory in mice

Adaptation of Optokinetic Reflex by Training with Different Frequency and Amplitude

Minhee Analysis Package: an integrated software package for detection and management of spontaneous synaptic events

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