Decreased Frequency of Functional Natural Interferon-Producing Cells in Peripheral Blood of Patients with the Acquired Immune Deficiency Syndrome

Howell, Donna; Feldman, Stephen B.; Kloser, Patricia; Fitzgerald-Bocarsly, Patricia

doi:10.1006/clin.1994.1076

Cited by 52 publications

(33 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In other studies, the defect in ex vivo production of IFN-a in HIV-1-infected patients has been described but its mechanism remains unclear [12][13][14][15][16][17]. This is the first report of an association between the profile of T-cell-derived cytokines and the production of IFN-a in HIV-1 infection.…”

Section: Discussionmentioning

confidence: 96%

“…We did not look at the number of circulating NIPC therefore we cannot exclude a reduced number of these cells in patients since a decreased frequency of NIPC, as well as a decreased quantity of IFN-a produced per IFN-a-producing cell, have been observed in HIV-1 infection [15]. There was no association between the impaired production of IFN-a and the plasma viral load, however, our results must be interpreted with caution considering the small number of patients in this study.…”

Section: Cytokines and Ifn-a In Hiv-1 Infection 439mentioning

confidence: 99%

See 1 more Smart Citation

Production of Interleukin‐4, Interferon (IFN)‐γ and IFN‐α in Human Immunodeficiency Virus‐1 Infection: An Imbalance of Type 1 and Type 2 Cytokines may Reduce the Synthesis of IFN‐α

Hober¹,

Benyoucef²,

Chehadeh³

et al. 1998

Scand J Immunol

View full text Add to dashboard Cite

Interferon‐α (IFN‐α) is an important molecule in the antiviral response, but cells from HIV‐1‐infected individuals show a reduced ability to secrete IFN‐α. We investigated an association between an imbalance of type 1/type 2 cytokines and the production of IFN‐α in HIV‐1 infection. We used whole blood culture to study the cytokine production profile, interferon‐γ (IFN‐γ) and interleukin‐4 (IL‐4), in response to HIV‐1 antigens and to study the Sendai Virus and HSV‐1‐induced‐production of IFN‐α in seven HIV‐1‐infected patients. An impaired synthesis of IFN‐α was obtained in patients with a predominant IL‐4 production (IL‐4 > IFN‐γ), and we found a positive correlation between the ex vivo production of IFN‐α and the IFN‐γ/IL‐4 ratio but not with the HIV RNA copy number in plasma. We investigated the role of T‐cell‐derived cytokines in the in vitro production of IFN‐α by PBMC from eight healthy donors, activated with Sendai Virus or HSV‐1. Whereas type 2 cytokines (IL‐4, IL‐13) inhibited virus‐induced IFN‐α synthesis, on the contrary, type 1 cytokines (IL‐2, IFN‐γ) enhanced it. A disarray in the T‐cell‐derived cytokine response may play a role in the defect of IFN‐α production in HIV‐1‐infected individuals. Further investigations are needed to explore this hypothesis.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Cytokines and Ifn-a In Hiv-1 Infection 439mentioning

confidence: 99%

Production of Interleukin‐4, Interferon (IFN)‐γ and IFN‐α in Human Immunodeficiency Virus‐1 Infection: An Imbalance of Type 1 and Type 2 Cytokines may Reduce the Synthesis of IFN‐α

Hober¹,

Benyoucef²,

Chehadeh³

et al. 1998

Scand J Immunol

View full text Add to dashboard Cite

show abstract

“…Induction of IFN-␣ secretion by a variety of stimuli is reduced in HIV infection (10,11,14), and recent studies demonstrated that CpG ODN-induced IFN-␣ production in PBMC from HIVϩ subjects was inversely related to plasma HIV RNA levels and correlated with CD4 FIG. 9.…”

Section: Discussionmentioning

confidence: 99%

Impaired Monocyte Maturation in Response to CpG Oligodeoxynucleotide Is Related to Viral RNA Levels in Human Immunodeficiency Virus Disease and Is at Least Partially Mediated by Deficiencies in Alpha/Beta Interferon Responsiveness and Production

Jiang¹,

Lederman²,

Salkowitz³

et al. 2005

J Virol

View full text Add to dashboard Cite

“…Some changes in peripheral blood PDC frequencies have also been ascribed to trafficking to lymph nodes during ongoing HIV replication. PDC isolated from HIV-1-infected patients also demonstrate functional abnormalities, including diminished production of IFN-␣ when exposed to viruses, including HIV-1 and herpes simplex virus in vitro (17,41), and these effects have since been ascribed to the functional defects of PDC (15,16,27). Control of HIV viral replication with effective antiretroviral therapy during early or chronic infection has been reported to partially restore the ability of PDC to respond to viruses by producing IFN-␣ (33,34,59).…”

Section: Human Immunodeficiency Virus Type 1 (Hiv-1) Infection Has Bementioning

confidence: 99%

Human Immunodeficiency Virus Viremia Induces Plasmacytoid Dendritic Cell Activation In Vivo and Diminished Alpha Interferon Production In Vitro

Tilton

Manion

Luskin

et al. 2008

J Virol

View full text Add to dashboard Cite

Human immunodeficiency virus type 1 (HIV-1) infection has been associated with perturbations of plasmacytoid dendritic cells (PDC), including diminished frequencies in the peripheral blood and reduced production of type I interferons (IFNs) in response to in vitro stimulation. However, recent data suggest a paradoxical increase in production of type 1 interferons in vivo in HIV-infected patients compared to uninfected controls. Using a flow cytometric assay to detect IFN-α-producing cells within unseparated peripheral blood mononuclear cells, we observed that short-term interruptions of antiretroviral therapy are sufficient to result in significantly reduced IFN-α production by PDC in vitro in response to CpG A ligands or inactivated HIV particles. The primary cause of diminished IFN-α production was reduced responsiveness of PDC to de novo stimulation, not diminished per cell IFN-α production or migration of cells to lymphoid organs. Real-time PCR analysis of purified PDC from patients prior to and during treatment interruptions revealed that active HIV-1 replication is associated with upregulation of type I IFN-stimulated gene expression. Treatment of hepatitis C virus-infected patients with IFN-α2b and ribavirin for hepatitis C virus infection resulted in a profound suppression of de novo IFN-α production in response to CpG A or inactivated HIV particles, similar to the response observed in HIV-infected patients. Together, these results suggest that diminished production of type I interferons in vitro by PDC from HIV-1-infected patients may not represent diminished interferon production in vivo. Rather, diminished function in vitro is likely a consequence of prior activation via type I interferons or HIV virions in vivo.

show abstract

Decreased Frequency of Functional Natural Interferon-Producing Cells in Peripheral Blood of Patients with the Acquired Immune Deficiency Syndrome

Cited by 52 publications

References 0 publications

Production of Interleukin‐4, Interferon (IFN)‐γ and IFN‐α in Human Immunodeficiency Virus‐1 Infection: An Imbalance of Type 1 and Type 2 Cytokines may Reduce the Synthesis of IFN‐α

Production of Interleukin‐4, Interferon (IFN)‐γ and IFN‐α in Human Immunodeficiency Virus‐1 Infection: An Imbalance of Type 1 and Type 2 Cytokines may Reduce the Synthesis of IFN‐α

Impaired Monocyte Maturation in Response to CpG Oligodeoxynucleotide Is Related to Viral RNA Levels in Human Immunodeficiency Virus Disease and Is at Least Partially Mediated by Deficiencies in Alpha/Beta Interferon Responsiveness and Production

Human Immunodeficiency Virus Viremia Induces Plasmacytoid Dendritic Cell Activation In Vivo and Diminished Alpha Interferon Production In Vitro

Contact Info

Product

Resources

About