Decreased fatty acids induced granulosa cell apoptosis in patients with diminished ovarian reserve

Zhao, Zhongying; Fan, Qigang; Zhu, Qinying; He, Ruifen; Li, Yang; Liu, Chang; Wang, Jing; Liang, Xiaolei

doi:10.1007/s10815-022-02462-8

Cited by 6 publications

(4 citation statements)

References 53 publications

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“…Fatty acids are important energy source, mainly providing ATP for oocytes through b-Oxidation, and inhibition of b-Oxidation lead to meiosis arrest of oocytes and development failure after fertilization (43,44). The decrease in fatty acids leads to DNA damage in granulosa cells and then accelerates cell apoptosis, which further reduces the quality of oocytes and embryos (45,46). We know that the imbalance of oxidation/antioxidation is an important reason for DOR (47).…”

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confidence: 99%

Metabonomic analysis of follicular fluid in patients with diminished ovarian reserve

Zhang

Wei

et al. 2023

Front. Endocrinol.

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BackgroundOvarian reserve is an important factor determining female reproductive potential. The number and quality of oocytes in patients with diminished ovarian reserve (DOR) are reduced, and even if in vitro fertilization-embryo transfer (IVF-ET) is used to assist their pregnancy, the clinical pregnancy rate and live birth rate are still low. Infertility caused by reduced ovarian reserve is still one of the most difficult clinical problems in the field of reproduction. Follicular fluid is the microenvironment for oocyte survival, and the metabolic characteristics of follicular fluid can be obtained by metabolomics technology. By analyzing the metabolic status of follicular fluid, we hope to find the metabolic factors that affect the quality of oocytes and find new diagnostic markers to provide clues for early detection and intervention of patients with DOR.MethodsIn this research, 26 infertile women with DOR and 28 volunteers with normal ovarian reserve receiving IVF/ET were recruited, and their follicular fluid samples were collected for a nontargeted metabonomic study. The orthogonal partial least squares discriminant analysis model was used to understand the separation trend of the two groups, KEGG was used to analyze the possible metabolic pathways involved in differential metabolites, and the random forest algorithm was used to establish the diagnostic model.Results12 upregulated and 32 downregulated differential metabolites were detected by metabolic analysis, mainly including amino acids, indoles, nucleosides, organic acids, steroids, phospholipids, fatty acyls, and organic oxygen compounds. Through KEGG analysis, these metabolites were mainly involved in aminoacyl-tRNA biosynthesis, tryptophan metabolism, pantothenate and CoA biosynthesis, and purine metabolism. The AUC value of the diagnostic model based on the top 10 metabolites was 0.9936.ConclusionThe follicular fluid of patients with DOR shows unique metabolic characteristics. These data can provide us with rich biochemical information and a research basis for exploring the pathogenesis of DOR and predicting ovarian reserve function.

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confidence: 99%

Metabonomic analysis of follicular fluid in patients with diminished ovarian reserve

Zhang

Wei

et al. 2023

Front. Endocrinol.

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“…The concentration of some fatty acids (i.e. pentadecanoic acid, heptadecanoic acid, cis-11-octadecenoic acid, cis-11-eicosenoic acid, cis,cis-11,14-eicosadienoic acid, and behenic acid, ect) were lower in DOR group with statistical significance, and the decreased transcription of HADHA (hydroxyacyl-coenzyme A dehydrogenase), ACSL (fatty acids β-oxidation related genes) were also decreased in DOR patients [ 57 ]. Therefore, further studies on the relationship between fatty acids and ovarian reserve are required.…”

Section: Hormonal Influence On Ovarian Reservementioning

confidence: 99%

Potential factors result in diminished ovarian reserve: a comprehensive review

Zhu,

Li,

et al. 2023

J Ovarian Res

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The ovarian reserve is defined as the quantity of oocytes stored in the ovary or the number of oocytes that can be recruited. Ovarian reserve can be affected by many factors, including hormones, metabolites, initial ovarian reserve, environmental problems, diseases, and medications, among others. With the trend of postponing of pregnancy in modern society, diminished ovarian reserve (DOR) has become one of the most common challenges in current clinical reproductive medicine. Attributed to its unclear mechanism and complex clinical features, it is difficult for physicians to administer targeted treatment. This review focuses on the factors associated with ovarian reserve and discusses the potential influences and pathogenic factors that may explain the possible mechanisms of DOR, which can be improved or built upon by subsequent researchers to verify, replicate, and establish further study findings, as well as for scientists to find new treatments.

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“…Factors such as age, body weight, ovarian reserve quantity and quality, unhealthy lifestyle choices, and exposure to environmental pollutants can all influence the likelihood of a successful outcome [ 10 , 12 , 13 ]. Among these factors, decreased ovarian reserve (DOR) presents a particularly formidable obstacle and frequently results in IVF failure [ 14 , 15 , 16 ]. Clinically, DOR is characterized by a decreased level of anti-Mullerian hormone (AMH), elevated levels of follicle-stimulating hormone (FSH), and/or a diminished antral follicle count (AFC) [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Analysis Reveals Association between Decreased Ovarian Reserve and In Vitro Fertilization Outcomes

An,

Zhang,

Zhu

et al. 2024

Metabolites

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In vitro fertilization (IVF) is a highly effective treatment for infertility; however, it poses challenges for women with decreased ovarian reserve (DOR). Despite the importance of understanding the impact of DOR on IVF outcomes, limited research has explored this relationship, particularly using omics approaches. Hence, we conducted a study to investigate the association between DOR and IVF outcomes, employing a metabolomic approach. We analyzed serum samples from 207 women undergoing IVF treatment, including 89 with DOR and 118 with normal ovarian reserve (NOR). Our findings revealed that DOR was significantly associated with unfavorable IVF outcomes, characterized by a reduced oocyte count, lower embryo quality, and decreased rates of pregnancy and live births. Furthermore, we identified 82 metabolites that displayed significant alterations in DOR patients, impacting diverse metabolic pathways. Notably, a distinct panel of metabolites, including palmitic acid, stearic acid, LysoPC(9:0(CHO)/0:0), PC(18:0/9:0(CHO)), and PC(16:0/9:0(CHO)), exhibited discriminatory power between the DOR and NOR groups, showcasing a strong correlation with IVF outcomes. These findings emphasize the crucial role of metabolomic disruptions in influencing IVF outcomes among women with DOR.

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Decreased fatty acids induced granulosa cell apoptosis in patients with diminished ovarian reserve

Cited by 6 publications

References 53 publications

Metabonomic analysis of follicular fluid in patients with diminished ovarian reserve

Metabonomic analysis of follicular fluid in patients with diminished ovarian reserve

Potential factors result in diminished ovarian reserve: a comprehensive review

Metabolomic Analysis Reveals Association between Decreased Ovarian Reserve and In Vitro Fertilization Outcomes

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