Decreased expression of <scp>SOX</scp>7 induces cell proliferation and invasion and correlates with poor prognosis in oral squamous cell carcinoma

Oh, Keunhee; Hong, Kyoung‐Ok; Huh, Young-Sung; Lee, Jae‐Il; Hong, Seong‐Doo

doi:10.1111/jop.12566

Cited by 12 publications

(15 citation statements)

References 21 publications

(41 reference statements)

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Section: Discussionsupporting

confidence: 89%

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SOX7 Blocks Vasculogenic Mimicry in Oral Squamous Cell Carcinoma

Hong

Yoon

et al. 2020

Preprint

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Background: Vasculogenic mimicry (VM) is the formation of an alternative circulatory system by aggressive tumor cells. The characteristics of VM and its underlying mechanism in oral squamous cell carcinoma (OSCC) remain unclear. This study aims to determine the relationship between VM channels in OSCC tissues and clinical outcomes and to investigate the biological role of SOX7 in VM in OSCC cells.Methods: CD31/PAS double staining was performed to evaluate VM status in OSCC tissue. The relationships between VM and clinicopathological variables, and VM and SOX7 levels were analyzed. The correlation between SOX7 levels and head and Neck cancer corhorts were investigated using in silico analysis.VM channel formation assay was performed to observe VM channels in the OSCC cell lines. To investigate the role of SOX7 in VM channel formation, SOX7 was transiently over-expressed in SCC-9 cells. VM-modulating genes were identified by Western blotting.Results: We confirmed the presence of VM channels in OSCC tissue and several cell lines and found a positive correlation between VM and lymph node metastasis and patient survival in OSCC (P = 0.003). In silico analysis from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database showed that down-regulation of SOX7 expression was significantly correlated with head and neck squamous cell carcinoma (HNSCC) patient cohorts (P = 0.0187) and lymph node metastasis (P = 0.0017). We also found that the presence of VM channels in OSCC tissue was inversely associated with SOX7 expression (P = 0.020). We observed that overexpression of SOX7 impaired VM channel formation by reducing the expression of VE-cadherin, thereby inhibiting cell migration and invasion.Conclusion: These results suggest that SOX7 plays an important role in the regulation of VM channel formation and may inhibit OSCC metastasis.

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Section: Discussionsupporting

confidence: 89%

“…We recently reported that expression of SOX7 was decreased in OSCC tissues compared with normal oral mucosal tissue. Moreover, we found that the absent SOX7 expression in patients with OSCC was signi cantly associated with lymph node metastasis and poor overall survival [17]. To further support our ndings, we performed in silico analysis.…”

Section: Resultssupporting

confidence: 54%

SOX7 Blocks Vasculogenic Mimicry in Oral Squamous Cell Carcinoma

Hong

Yoon

et al. 2020

Preprint

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“…We previously demonstrated that SOX7 expression was lower in OSCC tissue samples than in corresponding normal oral mucosal tissues and that the absence of SOX7 expression was signi cantly associated with advanced TNM stage, incidence of lymph node metastasis, and poor prognosis [17]. SOX7 expression was found to be frequently down-regulated in a variety of tumors; its forced expression inhibited cellular proliferation, migration, and invasion, suggesting that SOX7 functions as a tumor suppressor [29][30][31].…”

Section: Discussionmentioning

confidence: 99%

“…Formalin-xed, para n-embedded specimens were immunohistochemically stained using rabbit polyclonal anti-SOX7 (1:50; Santa Cruz Biotechnology, Santa Cruz, CA, USA) as previously described [17]. Immunohistochemical staining was assessed semi-quantitatively by two oral pathologists (KYO and SDH).…”

Section: Sox7 Immunohistochemistrymentioning

confidence: 99%

“…We recently reported that the expression of SOX7, a member of the sex-determining region Y-box (SOX) family, is associated with advanced TMN stage, lymph node metastasis, and poor overall survival in patients with OSCC [17]. Additionally, several studies have reported a tumor-inhibitory function of SOX7 associated with cancer regulatory pathways [18,19].…”

Section: Introductionmentioning

confidence: 99%

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SOX7 blocks vasculogenic mimicry in oral squamous cell carcinoma

Hong

Yoon

et al. 2020

Preprint

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Background : Vasculogenic mimicry (VM) is the formation of an alternative circulatory system by aggressive tumor cells. The characteristics of VM and its underlying mechanism in oral squamous cell carcinoma (OSCC) remain unclear. This study aims to determine the relationship between VM channels in OSCC tissues and clinical outcomes and to investigate the biological role of SOX7 in VM in OSCC cells. Methods : CD31/PAS double staining was performed to evaluate VM status in OSCC tissue. The relationships between VM and clinicopathological variables, and VM and SOX7 levels were analyzed. VM channel formation was assay performed to observe VM channels in the OSCC cell lines. To investigate the role of SOX7 in VM channel formation, SOX7 was transiently over-expressed in SCC-9 cells. VM-modulating genes were identified by Western blotting. Results : We confirmed the presence of VM channels in OSCC tissue and several cell lines and found a positive correlation between VM and lymph node metastasis and patient survival in OSCC ( P = 0.003). We also found that the presence of VM channels in OSCC tissue was inversely associated with SOX7 expression ( P = 0.020). We observed that overexpression of SOX7 impaired VM channel formation by reducing the expression of VE-cadherin, thereby inhibiting cell migration and invasion. Conclusion : These results suggest that SOX7 plays an important role in the regulation of VM channel formation and may inhibit OSCC metastasis.

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miR‐24‐3p promotes cell migration and proliferation in lung cancer by targeting SOX7

Yan

Zhu

et al. 2018

J of Cellular Biochemistry

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Lung cancer (LC) is one of the leading causes of cancer-related death in the world. miR-24-3p plays critical roles in many cancer types, including LC. In this study, we first investigated whether miR-24-3p promoted LC cell migration and proliferation in vitro. We used three bioinformatics algorithms to predict the miR-24-3p target gene to study the molecular mechanism by which miR-24-3p contributes to LC progression. Then, we used the luciferase reporter assay to identify whether SOX7 was a direct target of miR-24-3p. Moreover, Western blotting and a quantitative real time-polymerase chain reaction analysis showed that miR-24-3p downregulated SOX7 protein expression by a post-transcriptional mechanism. Finally, we determined that SOX7 had opposing effects to those of miR-24-3p on LC cell proliferation and migration, suggesting that miR-24-3p promotes cell proliferation and migration by directly targeting SOX7. Furthermore, miR-24-3p accelerated tumor growth in xenograft mice by targeting SOX7. These results provide the first clue that miR-24-3p could play a role as an oncomiR in LC by regulating SOX7.

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Decreased expression of SOX7 induces cell proliferation and invasion and correlates with poor prognosis in oral squamous cell carcinoma

Abstract: These results suggest that SOX7 inhibits cell proliferation, colony formation, and invasion in OSCC as a tumor suppressor and that negative SOX7 expression could be a poor prognostic indicator for patients with OSCC.

Cited by 12 publications

References 21 publications

SOX7 Blocks Vasculogenic Mimicry in Oral Squamous Cell Carcinoma

SOX7 Blocks Vasculogenic Mimicry in Oral Squamous Cell Carcinoma

SOX7 blocks vasculogenic mimicry in oral squamous cell carcinoma

miR‐24‐3p promotes cell migration and proliferation in lung cancer by targeting SOX7

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