Decreased expression of neuronal Per-Arnt-Sim domain protein 4 gene in the hippocampus of a post-stroke depression rat model

Zhao-hui, Zhang; Pengge, Fei; Mu, Junlin; Wang, Dan; Li, Wenqiang; Song, Jinggui

doi:10.3892/etm.2014.1537

Cited by 9 publications

(13 citation statements)

References 20 publications

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“…First, we were not able to assess risk factors such as nearly negative life accidents, family harmony, poverty and so on. Second, although we have acquired similar results in hippocampus of rats [23], few data supports Npas4 in PBMCs could represent its level in the brain, and we are not able to detect the level in human brain. Third, this study was conducted only in one hospital, but did not extend to other hospitals in China.…”

Section: Discussionmentioning

confidence: 63%

Decreased Npas4 expression in patients with post-stroke depression

Zhao

et al. 2019

Journal of Neurorestoratology

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Purpose:Post-stroke depression (PSD) is a frequent neuropsychiatric disorder following stroke which is associated with poor outcome. Neuronal Per-Arnt-Sim (PAS) domain protein 4 (Npas4) is associated with cognitive function. Npas4 expression in peripheral blood mononuclear cells (PBMCs) from patients with PSD was measured to find new therapeutic strategy.Patients and methods:Ischemic stroke patients (n = 152) within 1 week of stroke onset were recruited. At 3 months follow-up, the patients were divided into a PSD group (n = 77) and a stroke group (n = 75) using the Hamilton Rating Scale. Healthy subjects (n = 75) were also recruited in the study. The PSD group received 12 weeks of duloxetine treatment. Cognitive function was evaluated using the P300 test. Npas4 expression in PBMCs was measured by quantitative RT-PCR (qPCR).Results:Before treatment, P300 latencies in the PSD group were prolonged and the P300 amplitudes were lower than the control group (P < 0.01). Npas4 expression in the PSD group was also lower than the control group (P < 0.01). After treatment, the P300 latencies were reduced and the amplitudes were significantly elevated in the PSD group compared to that before treatment (P < 0.01). Meanwhile, Npas4 levels were significantly higher than that before treatment (P < 0.01). Npas4 expression was positively correlated to the P300 amplitudes (P < 0.05).Conclusion:Changes of Npas4 expression in PBMCs are associated with cognitive impairment in PSD patients and new therapeutic options applying Npas4-related transcript mechanism could be considered in the future.

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Section: Discussionmentioning

confidence: 63%

Decreased Npas4 expression in patients with post-stroke depression

Zhao

et al. 2019

Journal of Neurorestoratology

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“…Treatment with corticosterone or physical stress exposure down-regulates Npas4 expression in both prefrontal cortex and hippocampus in vivo [21, 32–34]. This may result from the activation of glucocorticoid pathways that suppress Npas4 expression [35].…”

Section: Behaviorally-induced Npas4 Expressionmentioning

confidence: 99%

Npas4: Linking Neuronal Activity to Memory

Sun

Lin

2016

Trends in Neurosciences

162

146

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Immediate early genes (IEGs) are rapidly activated after sensory and behavioral experience and are believed to be critical for converting experience into long-term memory. Neuronal PAS domain protein 4 (Npas4), a recently discovered IEG, has several characteristics that make it likely to be a particularly important molecular link between neuronal activity and memory: it is among the most rapidly induced IEGs, is expressed only in neurons, and is selectively induced by neuronal activity. By orchestrating distinct activity-dependent gene programs in different neuronal populations, Npas4 affects synaptic connections in excitatory and inhibitory neurons, neural circuit plasticity and memory formation. It may also be involved in circuit homeostasis through negative feedback and psychiatric disorders. We summarize these findings and discusses their implications.

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“…Rats were fasted overnight prior to the day of the experiment but were allowed free access to tap water. MCAO was conducted as previously described (25). Briefly, anesthesia was induced with 10% chloral hydrate [350 mg/kg BW, intraperitoneally (i.p.…”

Section: Methodsmentioning

confidence: 99%

“…Rats in the control and CUMS groups underwent the same surgery as above excluding threading of the nylon through the left CCA and left ECA. Following surgery, rats were selected according to Longa score, as previously described (25).…”

Section: Methodsmentioning

confidence: 99%

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Upregulation of aryl hydrocarbon receptor nuclear translocator 2 in the hippocampi of post‑stroke depression rats

et al. 2019

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Aryl hydrocarbon receptor nuclear translocator protein 2 (ARNT2), a member of the basic helix-loop-helix superfamily of transcription factors, may serve a vital role in neuronal survival and cell proliferation via formation of heterodimers with hypoxia-inducible factor-1α. Previous studies indicated that ARNT2 levels were elevated in the brains of ischemic rats; however, the involvement of ARNT2 in post-stroke depression (PSD) rats is not well understood. Therefore, the present study aimed to investigate the levels of ARNT2 in the hippocampi of PSD rats, and to clarify the potential association between ARNT2 and behavioral performance. A PSD rat model was established by middle cerebral artery occlusion (MCAO) followed by a 4-week chronic unpredictable mild stress (CUMS) regimen. A sucrose preference test and open field test (OFT) were conducted, and body weight was measured. In addition, reverse transcription-polymerase chain reaction and immunohistochemistry were performed to measure ARNT expression. Results indicated that MCAO+CUMS rats had lower weight gain, consumed less sucrose and moved less compared with controls. Furthermore, the mRNA and protein levels of ARNT in MCAO+CUMS rats were increased compared with in controls. The sucrose preference index and horizontal movement distance in the OFT were positively correlated with ARNT mRNA level. Thus, from these findings it was suggested that ARNT2 may be positively associated with improvement of cognitive impairment, and therefore may be a potential target in PSD treatment.

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Decreased expression of neuronal Per-Arnt-Sim domain protein 4 gene in the hippocampus of a post-stroke depression rat model

Cited by 9 publications

References 20 publications

Decreased Npas4 expression in patients with post-stroke depression

Decreased Npas4 expression in patients with post-stroke depression

Npas4: Linking Neuronal Activity to Memory

Upregulation of aryl hydrocarbon receptor nuclear translocator 2 in the hippocampi of post‑stroke depression rats

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