Decreased Expression of Leptin among Patients with Shoulder Stiffness

Ko, Jih‐Yang; Chen, Sung-Hsiung; Wu, Re‐Wen; Wu, Kuan-Ting; Hsu, Chieh-Cheng; Kuo, Sheng‐Fong

doi:10.3390/life12101588

Cited by 5 publications

(4 citation statements)

References 38 publications

(44 reference statements)

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“…The current study demonstrated that leptin expression was increased in capsule tissue of FS, suggesting that leptin might participate in the inflammatory and fibroblastic processes of FS, similar to its role in other fibrotic diseases. In contrast, Ko et al 25 showed that the expression of leptin was lower in the subacromial bursae and fluid of stiff shoulders. These findings appear to contradict the proinflammatory nature of leptin and the results of other fibrotic disorders.…”

Section: Discussionmentioning

confidence: 90%

Increased Expression of Adipokines in Patients With Frozen Shoulder

Qiao,

Yao,

Zhao

et al. 2023

Am J Sports Med

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Background: Adipokines represent a spectrum of bioactive molecules that could modulate fibroblastic and inflammatory processes. The role of adipokines in the pathogenesis of frozen shoulder (FS), a common musculoskeletal disorder characterized by chronic inflammation, remains obscure. Purpose: To evaluate whether adipokines contribute to the pathogenic mechanisms of FS and to evaluate any potential correlation of adipokines with patients’ symptoms. Study Design: Controlled laboratory study. Methods: Shoulder capsule specimens were obtained from 10 patients with FS and 10 patients with shoulder instability (control group). The specimens were dyed using hematoxylin and eosin and immunohistochemically assessed with antibodies targeting adipokines, collagen I, collagen III, and tumor necrosis factor α. Immunoreactivity was graded from “no” to “strong” in a blinded manner. Reverse transcription–quantitative real-time polymerase chain reaction (RT-qPCR) analysis was conducted. Before the surgery, patient-reported frequency of pain, severity of pain, stiffness, and shoulder range of motion were documented. Results: In comparison with the control group, patients with FS had significantly greater pain frequency, pain severity, and stiffness and more limited shoulder range of motion ( P < .001). Hematoxylin and eosin- and Masson trichrome–stained samples from the FS group displayed hypercellularity and increased collagen fibers. Immunohistochemistry and RT-qPCR analyses indicated that expression of adipokines was significantly increased in FS capsules compared with the control group. The expression of collagen I, collagen III, and tumor necrosis factor α was also increased in FS capsules. No significant correlation was noted between adipokine expression and patient-reported outcomes in the control group, whereas in patients with FS, adiponectin expression was correlated with pain frequency ( r = 0.78; P = .01) and stiffness ( r = 0.73; P = .02). Visfatin was also correlated with pain frequency ( r = 0.70; P = .02). Conclusion/Clinical Relevance: This study indicated a potential role for adipokines in the pathogenesis of FS and demonstrated a correlation between adipokine expression and patients’ pain and stiffness.

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Section: Discussionmentioning

confidence: 90%

Increased Expression of Adipokines in Patients With Frozen Shoulder

Qiao,

Yao,

Zhao

et al. 2023

Am J Sports Med

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“…The assessment of gene transcription levels was conducted employing the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay following our previous works (Ko et al 2022a , b , 2023a , b ). Tissues were pulverized under liquid nitrogen in the absence of RNase, and total RNA was extracted using the RiboPure RNA purification kit (Thermo Fisher Scientific, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Dickkopf-1 (DKK1) blockade mitigates osteogenesis imperfecta (OI) related bone disease

Ko,

Wang,

Lian

et al. 2024

Mol Med

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Background The current treatment of osteogenesis imperfecta (OI) is imperfect. Our study thus delves into the potential of using Dickkopf-1 antisense (DKK1-AS) to treat OI. Methods We analysed serum DKK1 levels and their correlation with lumbar spine and hip T-scores in OI patients. Comparative analyses were conducted involving bone marrow stromal cells (BMSCs) and bone tissues from wild-type mice, untreated OI mice, and OI mice treated with DKK1-ASor DKK1-sense (DKK1-S). Results Significant inverse correlations were noted between serum DKK1 levels and lumbar spine (correlation coefficient = − 0.679, p = 0.043) as well as hip T-scores (correlation coefficient = − 0.689, p = 0.042) in OI patients. DKK1-AS improved bone mineral density (p = 0.002), trabecular bone volume/total volume fraction (p < 0.001), trabecular separation (p = 0.010), trabecular thickness (p = 0.001), trabecular number (p < 0.001), and cortical thickness (p < 0.001) in OI mice. DKK1-AS enhanced the transcription of collagen 1α1, osteocalcin, runx2, and osterix in BMSC from OI mice (all p < 0.001), resulting in a higher von Kossa-stained matrix area (p < 0.001) in ex vivo osteogenesis assays. DKK1-AS also reduced osteoclast numbers (p < 0.001), increased β-catenin and T-cell factor 4 immunostaining reactivity (both p < 0.001), enhanced mineral apposition rate and bone formation rate per bone surface (both p < 0.001), and decreased osteoclast area (p < 0.001) in OI mice. DKK1-AS upregulated osteoprotegerin and downregulated nuclear factor-kappa B ligand transcription (both p < 0.001). Bone tissues from OI mice treated with DKK1-AS exhibited significantly higher breaking force compared to untreated OI mice (p < 0.001). Conclusions Our study elucidates that DKK1-AS has the capability to enhance bone mechanical properties, restore the transcription of osteogenic genes, promote osteogenesis, and inhibit osteoclastogenesis in OI mice.

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“…The mRNA expression levels of the assayed genes by the study group were expressed as 2 −∆∆Ct [23,24].…”

Section: Reverse Transcription-quantitative Polymerase Chain Reaction...mentioning

confidence: 99%

Micro Ribonucleic Acid−29a (miR−29a) Antagonist Normalizes Bone Metabolism in Osteogenesis Imperfecta (OI) Mice Model

Wang

Chen

et al. 2023

Biomedicines

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Osteogenesis imperfecta (OI) is not curative nowadays. This study tried to unriddle the therapeutic potential of micro ribonucleic acid-29a (miR-29a) antagonist in treating OI in a mouse animal model (B6C3Fe a/a-Col1a2oim/J). We showed that the expression levels of miR-29a were higher in bone tissues obtained from the OI mice than from wild-type mice demonstrated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and in situ hybridization assay. We established lentivirus-shuttled vector expressing miR-29a antisense oligonucleotide (miR-29a-AS) and miR-29a precursors (pre-miR-29a), showing that the inferior bony architecture in micro-computed tomography and pertinent morphometric parameters could be rescued by miR-29a-AS and deteriorated by pre-miR-29a. The decreased proliferating cell nuclear antigen (PCNA), increased Dickkopf-1 (DKK1), and decreased β-catenin expression in OI mice could be accentuated by pre-miR-29a and normalized by miR-29a-AS. The decreased osteogenesis and increased osteoclastogenesis in OI mice could also be accentuated by pre-miR-29a and normalized by miR-29a-AS. miR-29a-AS did not seem to possess severe hepatic or renal toxicities.

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Decreased Expression of Leptin among Patients with Shoulder Stiffness

Cited by 5 publications

References 38 publications

Increased Expression of Adipokines in Patients With Frozen Shoulder

Increased Expression of Adipokines in Patients With Frozen Shoulder

Dickkopf-1 (DKK1) blockade mitigates osteogenesis imperfecta (OI) related bone disease

Micro Ribonucleic Acid−29a (miR−29a) Antagonist Normalizes Bone Metabolism in Osteogenesis Imperfecta (OI) Mice Model

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