Decreased expression of hsa_circ_0001895 in human gastric cancer and its clinical significances

Abstract

Circular RNAs are a special class of endogenous RNAs characterized by jointing 3′ and 5′ ends together via exon or intron circularization. Recent studies found that circular RNAs are involved in the development of some human diseases. However, little is known about their roles in human gastric cancer. In this study, we chose hsa_circ_0001895 as a targeted circRNA to investigate its clinical significances in gastric cancer patients. Hsa_circ_0001895 expression levels in five gastric cancer cell lines and 257 specimens of tissues were measured by real-time quantitative reverse transcription polymerase chain reaction. Then, the potential relationship between hsa_circ_0001895 expression levels and patients' clinicopathological factors was investigated. A receiver operating characteristic curve was constructed for evaluating the diagnostic value of hsa_circ_0001895. Hsa_circ_0001895 expression levels in five detected gastric cancer cell lines (AGS, BGC-823, HGC-27, MGC-803, and SGC-7901) were all significantly downregulated than those in normal gastric epithelial GES-1 cells. Besides, compared with healthy control tissues, it was downregulated not only in 69.8% (67/96) gastric cancer tissues but also in gastric precancerous lesions. Moreover, hsa_circ_0001895 expression levels were significantly correlated with cell differentiation, Borrmann type, and tissue carcino-embryonic antigen expression. Our results suggested that hsa_circ_0001895 may play crucial roles during gastric cancerogenesis and is a potential biomarker for clinical prognosis prediction.

Functional role of circular RNAs in cancer development and progression

“…Moreover, in a cohort of 51 patients, hsa_circRNA_0067934 was significantly overexpressed in esophageal squamous cell carcinoma (ESCC) compared to the adjacent normal tissue and was associated with poor cellular differentiation [85]. Additionally, by combining results from 5 gastric cancer (GC) cell lines and 257 tumor tissue specimens it was found that hsa_circRNA_0001895 expression levels were significantly correlated with patient's clinicopathological factors suggesting its crucial role in gastric cancerogenesis and therefore is a potential biomarker for disease prognosis prediction [86]. In a similar study, hsa_circRNA_0003159 was shown to be downregulated in GC tissue compared to the adjacent normal tissue which was of diagnostic significance [87].…”

Diagnostic performance of circular RNAs in human cancers: A systematic review and meta‐analysis

“…Among the included publications, a total of 43 articles on 64 studies were analyzed involving 10,123 subjects in Table . Of the 64 studies, five studies focused on colorectal cancer (Ji et al, ; Wang, Li, et al, ; Wang et al, ; Zhang, Yang, et al, ; Zhuo, Lin, Chen, Huang, & Hu, ), 10 on breast cancer (Lu et al, ; Yin et al, ), 22 on gastric cancer (Chen, Li, Zhao, Xiao, & Guo, ; Huang, He, Liang, Huang, & Zhu, ; Li, et al, , ; Li, Shao, et al, ; Li, Song, et al, ; Lu et al, ; Lu, Shao, Ye, Xiao, & Guo, ; Shao, Chen, et al, ; Shao, Li, et al, ; Shao, Yang, et al, ; Sun, Tang, et al, ; Tian, Chen, Li, & Xiao, ; Wei et al, ; Xie et al, ; Zhao, Chen, Li, Xiao, & Zhang, ), four on lung cancer (Li, Wang, Chen, Chen, & Jin, ; Zhu et al, ; Zong et al, ), 16 on hepatocellular carcinoma (Chen, Zhang, Lin, Song, & Wang, ; Fu et al, , ; Li et al, ; Qin et al, ; Shang et al, ; Yao et al, ; Yao et al, ; Zhang, Zhang, Lin, & Wang, ; Zhang, Zhou, et al, ; Zhang, Xu, et al, ) and seven on other cancers (Fan, Cao, Liu, Zhang, & Li, ; Lan et al, ; Li et al, ; Sun, Li, et al, ; Teng et al, ; Wang, Xue, et al, ; Yang et al, ). Moreover, three articles with 13 studies explored the diagnostic value of the combined circRNAs (Chen, Li, Zhao, et al, ; Fan et al, ; Li, Shao, et al, ) in Table .…”

“…In the past decade, because of their special advantages as biomarkers, circRNAs have attracted attention in the detection, diagnosis, and prognosis of cancers (Chen, Li, Zheng, et al, ; Jiang et al, ; Shang et al, ; Zhang et al, ; Zhu et al, ). First, dysregulation of circRNAs was identified in tissues, plasma, and even exosomes from patients with many types of cancer (Li, Song, et al, ; Lu et al, ; Shao, Chen, et al, ). Second, circRNAs possess conserved sequences and close stable loop structures (Jeck et al, ; Qu et al, ).…”

“…Among the included publications, a total of 43 articles on 64 studies were analyzed involving 10,123 subjects in Table S1. Of the 64 studies, five studies focused on colorectal cancer (Ji et al, 2018;Wang, Li, et al, 2018;Wang et al, 2015;Zhang, Yang, et al, 2018;Zhuo, Lin, Chen, Huang, & Hu, 2017), 10 on breast cancer Yin et al, 2018), 22 on gastric cancer (Chen, Li, Zhao, Xiao, & Guo, 2017;Huang, He, Liang, Huang, & Zhu, 2017;Li, Shao, et al, 2018;Li, Song, et al, 2017;Lu et al, 2018;Shao, Chen, et al, 2017;Shao, Li, et al, 2017;Sun, Tang, et al, 2018;Tian, Chen, Li, & Xiao, 2018;Wei et al, 2019;Xie et al, 2018;Zhao, Chen, Li, Xiao, & Zhang, F I G U R E 1 The flow diagram of this meta-analysis 2018), four on lung cancer (Li, Wang, Chen, Chen, & Jin, 2018;Zhu et al, 2017;Zong et al, 2018), 16 on hepatocellular carcinoma (Chen, Zhang, Lin, Song, & Wang, 2018;Fu et al, 2018Fu et al, , 2017Li et al, 2019;Qin et al, 2016;Shang et al, 2016;Yao et al, 2017;Yao et al, 2018;Zhang, Zhou, et al, 2018; and seven on other cancers (Fan, Cao, Liu, Zhang, & Li, 2019;Lan et al, 2018;Li et al, 2019;Sun, Li, et al, 2018;…”

Circular RNA 0068669 as a new biomarker for hepatocellular carcinoma metastasis

“…circRNAs play a crucial role in various biologic processes such as cell development, proliferation, metastasis, fate decision, migration, and invasion. 3,4,[10][11][12][13] Hepatocellular carcinoma (HCC) is the third cancer-related death in the world. 14,15 Despite the improvement of diagnostic approaches and treatment strategies, the 5-year survival rate of HCC is still poor.…”