Decreased expression of CD36 in circumvallate taste buds of high-fat diet induced obese rats

Zhang, Xiaojuan; Zhou, Lihong; Ban, Xiang; Liu, Dianxin; Jiang, Wei; Liu, Xiaomin

doi:10.1016/j.acthis.2010.09.007

Cited by 73 publications

(68 citation statements)

References 31 publications

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“…In lean subjects, oral sensitivity to detect the taste of oleic acid is decreased after 4 weeks on a high-fat diet and increased after 4 weeks on a low-fat diet ( 20 ). These fi ndings are consistent with studies conducted in rodents showing that a high-fat diet decreases CD36 expression in taste buds cells ( 38,46 ) and reduces intestinal sensory sensitivity to the presence of fat ( 47 ). In obese subjects, oral sensitivity to oleic acid is unchanged after 4 weeks on a high-fat diet ( 20 ).…”

Section: Infl Uence Of Orlistat On Oleic Acid and Triolein Detection supporting

confidence: 80%

The fatty acid translocase gene CD36 and lingual lipase influence oral sensitivity to fat in obese subjects

Pepino

Love‐Gregory

Klein

et al. 2012

Journal of Lipid Research

244

226

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Obesity is caused by ingesting more energy than is expended over a long period of time. Dietary fat is the most energy-dense macronutrient, and its overconsumption has been linked to obesity ( 1-4 ). Obese people prefer foods with higher fat content ( 5 ), crave high-fat foods more frequently ( 6, 7 ), and consume more fat than lean individuals ( 8 ).Traditionally, perception of fat in the oral cavity is thought to rely almost entirely on textural and aromatic cues activating the somatosensory and olfactory systems. However, there is now increasing evidence to support an important role of the gustatory system in fat perception ( 9-20 ) as well as in intestinal lipid metabolism ( 10, 21 ). Oral and gastrointestinal fat sensory sensitivity appear to be associated ( 16 ) and there is similarity in the chemosensory reception events and their signaling transduction pathways in the tongue and gastrointestinal tract ( 21 ). An important requirement for the involvement of a gustatory component in dietary lipid detection is the hydrolysis of triacylglycerols (TGs) to release free FAs, the signaling stimulus, as was demonstrated through the use of the lipase inhibitor orlistat ( 22 ). In rodents, lingual lipase is essential for the gustatory perception of dietary fats ( 22 ) and the addition of orlistat to fat emulsions diminishes the rat's preference for TG, but not FA ( 22 ). Although it is not known whether lingual lipase is important for oral fat perception in humans, data from a recent study suggests that lingual lipase lipolytic activity can produce FA within the concentration range required to activate oral sensors ( 18 ).Several putative fat taste receptor classes have been identifi ed in rodents ( 12,23,24 ), including the glycoprotein CD36 ( 25 ). The presence of CD36, a scavenger receptor that mediates uptake and traffi cking of lipids in diverse cell types ( 26 ), has been documented in the gustatory papillae of rodents ( 25, 27 ), pigs, and humans ( 28 ). In rodents, the interaction between CD36 and FA results in signaling events that depend on an intact neuronal gustatory pathway ( 15,29 ). CD36 gene knockout impedes fat detection in mice without affecting sweet or bitter perception Abstract The precise orosensory inputs engaged for dietary lipids detection in humans are unknown. We evaluated whether a common single nucleotide polymorphism (rs1761667) in the CD36 gene that reduces CD36 expression and the addition of orlistat, a lipase inhibitor, to reduce FA release from triacylglycerols (TGs), the main component of dietary fats, would attenuate fat orosensory sensitivity in humans. Twenty-one obese subjects with different rs1761667 genotypes (6 AA, 7 AG, and 8 GG) were studied on two occasions in which oleic acid and triolein orosensory detection thresholds were measured using emulsions prepared with and without orlistat. Subjects homozygous for the G-allele had 8-fold lower oral detection thresholds for oleic acid and triolein than subjects homozygous for the A allele, which associates with lower CD36 expr...

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Section: Infl Uence Of Orlistat On Oleic Acid and Triolein Detection supporting

confidence: 80%

The fatty acid translocase gene CD36 and lingual lipase influence oral sensitivity to fat in obese subjects

Pepino

Love‐Gregory

Klein

et al. 2012

Journal of Lipid Research

244

226

View full text Add to dashboard Cite

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“…5 Based on fundamental similarities in receptor-mediated fatty acid detection within taste receptor cells and enteroendocrine cells of GI tract (CD36 Fat intake modulates fat taste sensitivity JE Stewart and RSJ Keast and G protein receptors 120, 40 and 43), 8,9 it is suspected that oral fatty acid detection and adaptations to fat exposure would mirror events that occur in the GI tract. Indeed modulation of fatty acid taste receptor expression has recently been reported in rodents exposed to a high-fat diet, 25 elucidating that physical changes in taste receptor expression can be induced by a high-fat diet.…”

Section: Discussionmentioning

confidence: 99%

Recent fat intake modulates fat taste sensitivity in lean and overweight subjects

2011

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Objective: To evaluate the effects of a high-fat and low-fat diet on taste sensitivity to oleic acid (C18:1) in lean and overweight/ obese (OW/OB) subjects. Design: Randomized cross-over dietary intervention involving the consumption of a high-fat (445% fat) and low-fat (o20% fat) diet, both consumed over a 4-week period. Subjects: A total of 19 lean, mean age 33±13 years, mean body mass index (BMI) 23.2±2.2 kg m -2 and 12 OW/OB, mean age 39.5 ± 3 years, mean BMI 28 ± 2.6 kg m -2 , subjects participated in the study, which measured taste thresholds for C18:1, fat perception and hedonic ratings for regular (RF) and lowered-fat (LF) foods before, and following consumption of a high-and low-fat diet. Results: Consumption of the low-fat diet increased taste sensitivity to C18:1 among lean and OW/OB subjects (Po0.05) and increased the subjects ability to perceive small differences in the fat content of custard (P ¼ 0.05). Consumption of the high-fat diet significantly decreased taste sensitivity to C18:1 among lean subjects (Po0.05), with no change in sensitivity among OW/OB persons (P ¼ 0.609). The hedonic ratings for several RF and LF foods differed following the diets. Conclusion: Alterations in the fat content of the diet modulated taste sensitivity to C18:1 among lean subjects, which was increased following a 4-week period of fat restriction and attenuated following the high-fat diet. The failure of the high-fat diet to alter fatty acid taste thresholds among OW/OB subjects suggests that these individuals were 'adapted' to high-fat exposure, perhaps because of differences in habitual fat consumption. Taken together, these data suggest that excessive dietary fat attenuates nutrient sensing epithelia response in the oral cavity, which could be associated with changes in diet and weight status.

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confidence: 99%

“…These mice are protected from weight gain when eating a HFD (1, 10 -12, 17, 21, 41). In the mouth, CD36 has been proposed as the taste receptor for dietary fat and/or fatty acids and is primarily expressed on Type II taste cells on the circumvallate papillae of the tongue (12,21,29,42,51).Several studies have been conducted to elucidate differences in the detection of fatty acids in taste receptor cells in animal models that differ in their susceptibility to obesity (15,16). These studies utilized obesity-prone Osborne-Mendel rats (OM) and obesity-resistant S5B/Pl rats (S5B).…”

mentioning

confidence: 99%

Preference for linoleic acid in obesity-prone and obesity-resistant rats is attenuated by the reduction of CD36 on the tongue

Chen

Bench

Schreiber

et al. 2013

American Journal of Physiology-Regulatory, Integrative and Comp

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Chen CS, Bench EM, Allerton TD, Schreiber AL, Arceneaux KP 3rd, Primeaux SD. Preference for linoleic acid in obesity-prone and obesityresistant rats is attenuated by the reduction of CD36 on the tongue. Am J Physiol Regul Integr Comp Physiol 305: R1346-R1355, 2013. First published October 23, 2013 doi:10.1152/ajpregu.00582.2012.-Differential sensing of dietary fat and fatty acids by the oral cavity is proposed to regulate the susceptibility to obesity. In the current experiments, animals that differ in their susceptibility to obesity were used to investigate the influence of the oral cavity on the preference for the polyunsaturated fatty acid, linoleic acid. In experiment 1, the preference for differing concentrations of linoleic acid was determined in obesity-prone Osborne-Mendel (OM) and obesity-resistant S5B/Pl (S5B) rats. The preference threshold for linoleic acid was lower in S5B rats, compared with OM rats. To determine whether differences in linoleic acid preference threshold were related to innate strain differences in the fatty acid receptors on the tongue, the expression of GPR120, GPR40, and CD36 on the circumvallate papillae were assessed in OM and S5B rats. Results indicated that the expression of CD36, GPR40, and GPR120 did not differ between these two strains. Numerous studies have examined the role of CD36 on fat intake; therefore, in experiment 3, RNA interference was used to decrease the expression of CD36 on the tongues of OM and S5B rats, and the effect of decreased CD36 expression on linoleic acid preference was determined. CD36 siRNA attenuated linoleic acid preference for the most preferred concentration in both OM and S5B rats. Overall, these data indicate that there are innate differences in the preference threshold for linoleic acid in obesity-prone and obesity-resistant rats. Experimentally reducing the expression of CD36 on the circumvallate papillae attenuated the preference for linoleic acid in both strains. preference threshold; obesity-prone; obesity-resistant; CD36; taste CONSUMPTION OF AN ENERGY-DENSE, high-fat diet has been associated with an increased risk for developing obesity (23). The detection of dietary fat and subsequent fat intake are mediated by input from the oral cavity and by postingestive effects (12). The ability to sense dietary fat in the oral cavity has two main functions: 1) mediation of the cephalic phase response and 2) regulation of food intake (12, 31). Differences in fat/fatty acid sensors in the oral cavity have been proposed as potential mechanisms contributing to the susceptibility of becoming obese. This is characterized by a subset of individuals who become obese on a high-fat diet (HFD) and another subset of individuals that are resistant to becoming obese when consuming a HFD (15,24,31,33). Understanding the detection and regulation of fat intake by fat/fatty acid sensors in the oral cavity may elucidate the mechanisms by which individuals develop preferences for high-fat foods, overconsume dietary fat, and subsequently become obese.Demonstration...

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Decreased expression of CD36 in circumvallate taste buds of high-fat diet induced obese rats

Cited by 73 publications

References 31 publications

The fatty acid translocase gene CD36 and lingual lipase influence oral sensitivity to fat in obese subjects

The fatty acid translocase gene CD36 and lingual lipase influence oral sensitivity to fat in obese subjects

Recent fat intake modulates fat taste sensitivity in lean and overweight subjects

Preference for linoleic acid in obesity-prone and obesity-resistant rats is attenuated by the reduction of CD36 on the tongue

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