Decreased embryo implantation in rabbits infected with Taenia pisiformis

Domínguez-Roldan, Rosa; Arias-Hernández, David; Dunstand-Guzmán, Emmanuel; Sciutto, Edda; Aguirre-Flores, Virginio; Flores-Pérez, Iván; Hallal-Calleros, Claudia

doi:10.1007/s00436-022-07694-2

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…2 Additionally, this disease can lead to serious liver injury, ascites, weight loss, and reduced fecundity in infected animals. 3,4 Alveolar echinococcosis (AE), caused by E. multilocularis, primarily affects the liver of both humans and animals, resulting in conditions such as fibrosis, cirrhosis, cancer, and even death. 5,6 Therefore, early diagnosis of metacestodiasis plays a pivotal role in controlling disease progression and ensuring the health of humans and animals.…”

Section: Introductionmentioning

confidence: 99%

“…T. pisiformis is highly prevalent in China, with infection rates of 20%–100% and mortality rates of 4%–23.69% in rabbits 2 . Additionally, this disease can lead to serious liver injury, ascites, weight loss, and reduced fecundity in infected animals 3,4 . Alveolar echinococcosis (AE), caused by E. multilocularis , primarily affects the liver of both humans and animals, resulting in conditions such as fibrosis, cirrhosis, cancer, and even death 5,6 .…”

Section: Introductionmentioning

confidence: 99%

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Parasite‐derived microRNA let‐7‐5p detection for metacestodiasis based on rolling circular amplification‐assisted CRISPR/Cas9

Wang,

Pu,

Liu

et al. 2024

The FASEB Journal

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Metacestodiasis is an infectious disease caused by the larval stage of cestode parasites. This disease poses a serious health hazard to wildlife, livestock, and humans, and it incurs substantial economic losses by impacting the safety of the livestock industry, the quality of meat production, and public health security. Unfortunately, there is currently no available molecular diagnostic method capable of distinguishing cysticercus‐ and Echinococcus‐derived microRNAs (miRNAs) from other helminthes and hosts in the plasma of metacestode‐infected animals. This study aims to develop a specific, sensitive, and cost‐efficient molecular diagnostic method for cysticercosis and echinococcosis, particularly for early detection. The study developed a rolling circular amplification (RCA)‐assisted CRISPR/Cas9 detection method based on parasite‐derived miRNA let‐7‐5p. Using a series of dilutions of the let‐7 standard, the limit of detection (LOD) of the qPCR, RCA, and RCA‐assisted CRISPR/Cas9 methods was compared. The specificity of qPCR and CRISPR/Cas9 was evaluated using four artificially synthesized let‐7 standards from different species. A total of 151 plasma samples were used to evaluate the diagnostic performance. Additionally, the study also assessed the correlation between plasma levels of let‐7‐5p, the number of Taenia pisiformis cysticerci, and the weight of Echinococcus multilocularis cysts. The results demonstrated that the RCA‐assisted CRISPR/Cas9 assay could significantly distinguish let‐7 from cestodes and other species, achieving a LOD of 10 aM; the diagnostic sensitivity and specificity for rabbit cysticercosis and mouse E. multilocularis were 100% and 97.67%, and 100% and 100%, respectively. Notably, let‐7‐5p gradually increased in the plasma of T. pisiformis‐infected rabbits from 15 days post infection (dpi), peaked at 60 dpi, and persisted until 120 dpi. In E. multilocularis‐infected mice, let‐7‐5p gradually increased from 15 dpi and persisted until 90 dpi. Furthermore, the expression of let‐7‐5p positively correlated with the number of cysticerci and cyst weight. These results indicated that the let‐7‐5p‐based RCA‐assisted CRISPR/Cas9 assay is a sensitive and specific detection method that can be used as a universal diagnostic method for metacestodiasis, particularly for early diagnosis (15 dpi).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Parasite‐derived microRNA let‐7‐5p detection for metacestodiasis based on rolling circular amplification‐assisted CRISPR/Cas9

Wang,

Pu,

Liu

et al. 2024

The FASEB Journal

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Major Endoparasitoses in Rabbits: oxyuriosis and Metacestodosis

Simões,

Lopes

2024

Veterinary Care of Farm Rabbits

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Effect of infestation with Psoroptes cuniculi on reproduction and behavior of obese rabbit does (Oryctolagus cuniculi)

Arjona-Jiménez,

Flores-Pérez,

Valdez-Torres

et al. 2024

PLoS ONE

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Parasites and obesity are health problems worldwide. Rabbits are production animals yielding one of the healthiest meats, also taking advantage of skin, hair, limbs and excreta. It is among the most frequent pets in some countries and widely used as animal model in research. Psoroptes cuniculi is a mite of high transmission rate, affecting welfare and production and obesity causes multiple metabolic, endocrine and immunological disorders, being an emerging problem in domestic animals. Obesity and acarosis are prolonged stressors, modifying the activity of the hypothalamic-pituitary-adrenal axis that can induce metabolic and behavioral disorders. Alterations caused by comorbidities could be similar to or different from those induced by each morbidity separately. We analyzed the influence of obesity on the infection degree with P. cuniculi and on behavior and production. Rabbit does induced to obesity were infected and mated; behaviors in the open field test, obesity estimation indices and productive parameters at delivery and weaning were analyzed. The acarosis induced a decrease in feed intake and a decrease in body weight, a decrease in locomotor, exploratory and chinning behaviors in normal weight and obese does. The infection induced 23% mortality at birth, obesity 45% and comorbidity 74%, while in normal weight rabbits a 6.5% was observed. Weight gain from birth to weaning was lower in the comorbid group, reaching a litter weight of 4.5±0.13 kg in healthy normal weight does and 2.6±0.67 kg in comorbid does. The disturbances induced by the comorbidity were magnified in both behavioral and productive parameters.

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Decreased embryo implantation in rabbits infected with Taenia pisiformis

Cited by 3 publications

References 12 publications

Parasite‐derived microRNA let‐7‐5p detection for metacestodiasis based on rolling circular amplification‐assisted CRISPR/Cas9

Parasite‐derived microRNA let‐7‐5p detection for metacestodiasis based on rolling circular amplification‐assisted CRISPR/Cas9

Major Endoparasitoses in Rabbits: oxyuriosis and Metacestodosis

Effect of infestation with Psoroptes cuniculi on reproduction and behavior of obese rabbit does (Oryctolagus cuniculi)

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