Decreased D-serine levels in CSF of schizophrenia patients

Amar, Shirly; Bendikov, Inna; Nadri, Carmit; Panizzutti, Rogério; Miranda, Joari De; Wolosker, Herman; Agam, Galila

doi:10.1016/s0924-977x(06)80013-1

Cited by 1 publication

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“…1), was newly synthesized in addition to R-NPCA, and the enantiomeric separation of a racemic compound was investigated using HPLC with ECD following diastereomeric derivatization with R-NPCA or S-NPCA. D,L-Serine was chosen as a racemic compound, because the determination of D-serine in biological fluids is a crucial subject for the treatment of schizophrenia (Hashimoto et al, 2003;Amar et al, 2006;Burnet et al, 2008); further, it might contribute to diagnosis and/or pharmacological therapeutics of schizophrenia. In order to detect Dserine in biosamples, HPLC with a chiral column has also been useful ; however, an appropriate and expensive chiral column is required for the achievement of enantiomeric separation.…”

Section: Introductionmentioning

confidence: 99%

Enantiomeric separation of d‐ and l‐serine using high‐performance liquid chromatography with electrochemical detection following pre‐column diastereomer derivatization

Yamashita

Fukushima

Sasaki

et al. 2009

Biomedical Chromatography

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Enantiomeric separation of D- and L-serine on an octadecylsilica column was investigated using (2R)-2,5-dioxopyrrolidin-1-yl-2,5,7,8-tetramethyl-6-(tetrahydro-2H-pyran-2-yloxy)chroman-2-carboxylate (R-NPCA), which was developed for a pre-column derivatization reagent for electrochemical detection. In addition, (2S)-2,5-dioxopyrrolidin-1-yl-2,5,7,8-tetramethyl-6-(tetrahydro-2H-pyran-2-yloxy)chroman-2-carboxylate (S-NPCA) was newly synthesized from (S)-(-)-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (S-alpha-CA), and the enantiomeric separation of D- and L-serine using S-NPCA was also examined. The enantiomeric separation of D,L-serine was achieved using the R- or S-NPCA as a chiral derivatization reagent, and the elution orders of the enantiomers were reversed between R- and S-NPCA. The elution orders of D- and L-serine unexpectedly reversed between the phosphate buffer at pH 4.0 and pH 2.2, both of which were used in the mobile phase. Separation factors obtained using R- and S-NPCA were similar-1.09 and 1.07, respectively. The detection limit was approximately 940 fmol on the column (signal-to-noise ratio 3) when the applied voltage was +650 mV.

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Section: Introductionmentioning

confidence: 99%