Decreased Anti-Müllerian hormone and Anti-Müllerian hormone receptor type 2          in hypothalami of old Japanese Black cows

Kereilwe, Onalenna; Kadokawa, Hiroya

doi:10.1292/jvms.20-0159

Cited by 2 publications

(4 citation statements)

References 14 publications

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“…We obtained brain samples (cortex, hippocampus, anterior hypothalamus containing the SCN [29][30][31] and in Supplementary Figure 1. The frontal lobe cortex was collected caudal to the central sulcus near the midline.…”

Section: Animals and Treatmentsmentioning

confidence: 99%

“…We used a previously reported method, with minor modifications, for western blotting of bovine brain [30,31]. Briefly, proteins were extracted from the frozen stock tissues of young and old groups (n = 6 per region per group) using a tissue protein extraction reagent (T-PER; Thermo Fisher Scientific) with a Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific).…”

Section: Western Blotting For -Synuclein Detectionmentioning

confidence: 99%

“…Free-floating tissue sections were permeabilized with PBS containing 0.5% Tween 20 for 3 min. We combined two quenching methods, glycine/hydrogen peroxide [30,31] and Vector True VIEW autofluorescence quenching (VTVAQ) kit (Vector Laboratories Inc., Burlingame, CA, USA). Briefly, the tissue was blocked with PBS containing 2% normal goat serum, 50 mM glycine, 0.05% Tween 20, 0.1% Triton X 100, and 0.1% BSA for 30 min [30].…”

Section: Fluorescent Immunohistochemistry and Confocal Microscopymentioning

confidence: 99%

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Alpha-synuclein expression in oxytocin neurons of young and old bovine brains

NIYONZIMA,

ASATO,

KADOKAWA

2024

J. Reprod. Dev.

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Understanding of central nervous system mechanisms underlying age-related infertility remains limited. Fibril -synuclein, distinct from its monomeric form, is implicated in age-related diseases.Notably, fibril -synuclein spreads among neurons, similar to prions, from damaged old neurons in cortex and hippocampus to healthy neurons. However, less is known whether -synuclein propagates into oxytocin neurons, which play crucial roles in reproduction. We compared -synuclein expression in the oxytocin neurons in suprachiasmatic nucleus (SCN), supraoptic nucleus (SON), paraventricular hypothalamic nucleus (PVN), and posterior pituitary (PP) gland of healthy heifers and aged cows to determine its role in age-related infertility. We analyzed mRNA and protein expression, along with Congo red histochemistry and fluorescent immunohistochemistry for oxytocin and -synuclein, followed by confocal microscopy with Congo red staining. Both mRNA and protein expressions of synuclein were confirmed in the bovine cortex, hippocampus, SCN, SON, PVN, and PP tissues.Significant differences in -synuclein mRNA expressions were observed in the cortex and hippocampus between young heifers and old cows. Western blots showed five bands of -synuclein, probably reflecting monomers, dimers, and oligomers, in the cortex, hippocampus, SCN, SON, PVN, and PP tissues, and there were significant differences in some bands between the young heifers and old cows.Bright-field and polarized light microscopy did not detect obvious amyloid deposition in the aged hypothalami; however, higher-sensitive confocal microscopy unveiled strong positive signals for Congo red and -synuclein in oxytocin neurons in the aged hypothalami. -synuclein was expressed in oxytocin neurons, and some differences were observed between young and old hypothalami.

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Section: Animals and Treatmentsmentioning

confidence: 99%

Section: Western Blotting For -Synuclein Detectionmentioning

confidence: 99%

Section: Fluorescent Immunohistochemistry and Confocal Microscopymentioning

confidence: 99%

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Alpha-synuclein expression in oxytocin neurons of young and old bovine brains

NIYONZIMA,

ASATO,

KADOKAWA

2024

J. Reprod. Dev.

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“…Old cows were slaughtered after parturition a sufficient number of times as planned by farmers to obtain beef, usually after 84 months of age. We obtained whole brains (including the hypothalamus) for EPl extraction from healthy, post-pubertal, young Japanese Black heifers (2.2 ± 0.0 years old, n = 6) and old Japanese Black cows (10.3 ± 1.6 years old, n = 6) from the local abattoir, and we preserved these without air at À80 C. We obtained hypothalami samples for RNAseq from different healthy, post-pubertal, non-pregnant, non-lactating Japanese Black heifers (2.2 ± 0.0 years old, n = 5) and from old Japanese Black cows (10.3 ± 1.6 years old; 7.4 ± 1.1 parity; n = 5), according to the method reported previously (Kereilwe & Kadokawa, 2020a). Briefly, brain tissues were dissected as follows: rostral border of the optic chiasm, caudally rostral to the mammillary bodies, lateral to the optic chiasm, and 0.5 cm dorsal to the third ventricle.…”

Section: Sample Collectionmentioning

confidence: 99%

Ethanolamine plasmalogens derived from whale brain stimulate both follicle‐stimulating hormone and luteinizing hormone secretion by bovine gonadotrophs

Saito

Bando²,

Kotaniguchi³

et al. 2023

Animal Science Journal

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Ethanolamine plasmalogens (EPls) are the only known ligands of a novel receptor, G protein‐coupled receptor 61, and bovine brain EPls stimulate follicle‐stimulating hormone (FSH) but not luteinizing hormone (LH), secreted by bovine gonadotrophs. We hypothesized that the brain EPls of whales (Balaenoptera edeni), another Cetartiodactyla with at least twice the lifespan of bovines, could stimulate FSH secretion by gonadotrophs. To test this hypothesis, bovine gonadotrophs (from approximately 2‐year‐old Japanese Black heifers) were cultured for 3.5 days and treated with increasing concentrations of brain EP1s from whales (approximately 22 years old). FSH and LH secretion was stimulated by all tested concentrations of whale EPls (p < 0.05). To clarify the important differences between bovine and whale EPls, we utilized two‐dimensional liquid chromatography–mass spectrometry, which revealed 35 peaks. Among them, we observed significant differences between 12 EPl molecular species. Additionally, we identified differentially expressed genes for enzymes involved in EPl synthesis or degradation in the hypothalamus of young heifers and old cows (approximately 10 years old) as compared to whales (approximately 28 years old) via deep sequencing of the transcriptome. We conclude that whale brains contain unique EPls that stimulate both FSH and LH secretion by bovine gonadotrophs.

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Decreased Anti-Müllerian hormone and Anti-Müllerian hormone receptor type 2 in hypothalami of old Japanese Black cows

Cited by 2 publications

References 14 publications

Alpha-synuclein expression in oxytocin neurons of young and old bovine brains

Alpha-synuclein expression in oxytocin neurons of young and old bovine brains

Ethanolamine plasmalogens derived from whale brain stimulate both follicle‐stimulating hormone and luteinizing hormone secretion by bovine gonadotrophs

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