Decrease of the DNA methylation levels of the ADRB3 gene in leukocytes is related with serum folate in eutrophic adults

Abstract

BackgroundDNA methylation has been evidenced as a potential epigenetic mechanism related to various candidate genes to development of obesity. Therefore, the objective of this study was to evaluate the DNA methylation levels of the ADRB3 gene by body mass index (BMI) in a representative adult population, besides characterizing this population as to the lipid profile, oxidative stress and food intake.MethodsThis was a cross-sectional population-based study, involving 262 adults aged 20–59 years, of both genders, representative of the East and West regions of the municipality of João Pessoa, Paraíba state, Brazil, in that were evaluated lifestyle variables and performed nutritional, biochemical evaluation and DNA methylation levels of the ADRB3 gene using high resolution melting method. The relationship between the study variables was performed using analyses of variance and multiple regression models. All results were obtained using the software R, 3.3.2.ResultsFrom the stratification of categories BMI, was observed a difference in the average variables values of age, waist-to-height ratio, waist-to-hip ratio, waist circumference, triglycerides and intake of trans fat, which occurred more frequently between the categories “eutrophic” and “obesity”. From the multiple regression analysis in the group of eutrophic adults, it was observed a negative relationship between methylation levels of the ADRB3 gene with serum levels of folic acid. However, no significant relation was observed among lipid profile, oxidative stress and food intake in individuals distributed in the three categories of BMI.ConclusionsA negative relationship was demonstrated between methylation levels of the ADRB3 gene in eutrophic adults individuals with serum levels of folic acid, as well as with the independent gender of BMI, however, was not observed relation with lipid profile, oxidative stress and variables of food intake. Regarding the absence of relationship with methylation levels of the ADRB3 gene in the categories of overweight, mild and moderate obesity, the answer probably lies in the insufficient amount of body fat to initiate inflammatory processes and oxidative stress with a direct impact on methylation levels, what is differently is found most of the times in exacerbated levels in severe obesity.Electronic supplementary materialThe online version of this article (10.1186/s12967-018-1529-0) contains supplementary material, which is available to authorized users.

Dietary intakes and biomarker patterns of folate, vitamin B6, and vitamin B12 can be associated with cognitive impairment by hypermethylation of redox-related genes NUDT15 and TXNRD1

“…It is well documented that folate in one-carbon metabolism plays a central role in the synthesis, repair, and methylation of DNA, where it acts as a methyl donor. Folate homeostasis disruption could affect methylation potential through DNA gene hyper- or hypomethylation reactions and lead to gene transcription alterations (overexpression and/or gene silencing) [50, 51]. In our discovery group, genome-wide DNA methylation analysis identified that folate was significantly associated with DNA methylation of antioxidant genes NUDT15 and TXNRD1, both of which were hypermethylated in the MCI group.…”

Methylation profile of the ADRB3 gene and its association with lipid profile and nutritional status in adults

“…In the study conducted by Oliveira et al [38], it was observed that higher levels of methylation were not associated with lipid profile and oxidative stress, when the individuals were separated according to eutrophic, overweight and obesity classification, the study used the analysis of methylation levels by real-time PCR method, MS-HRM, in addition in the present study the whole sample was used.…”

The direct correlation between oxidative stress and LDL-C levels in adults is maintained by the Friedewald and Martin equations, but the methylation levels in the MTHFR and ADRB3 genes differ

“…This information and other information about the sampling are further detailed in recently published studies [ 39 , 40 ], with the exception of the levels of methylation of the LPL and MTHFR gene, the calculated values of LDL-C in accordance with Martin [ 13 ] and the stratification of the sample according to the LDL-C values.…”