Decrease of serum biomarker of type II Collagen degradation (Coll2-1) by intra-articular injection of an autologous plasma-rich-platelet in patients with unilateral primary knee osteoarthritis

Abstract

Objective: This study aimed to evaluate the effect of one dose of intra-articular injection of (PRP) in the knee joint on a specific osteoarthritis (OA) serum biomarker of cartilage degeneration, Collagen 2-1 (Coll2-1), over a short period of 3 months. The aim extended to clarify the effect of PRP on the functional status of the osteoarthritic knee joint. Material and Methods:Sixty patients with primary unilateral knee OA were enrolled in this study. They were subdivided according to Kellgren-Lawrence grading scale (KL) into (Group Ι): including patients with KL grade < 3 and (Group ΙΙ): including patients with KL grade ≥3. Patients were asked to complete the Western Ontario and McMaster Universities Arthritis Index (WOMAC) Score. PRP was prepared and injected immediately into the affected knee. Serum Coll2-1 (S.Coll2-1) concentration was measured by enzyme-linked immunosorbent assay (ELISA) kit pre and 3 months after PRP injection.Results: Significant reduction in S.Coll2-1 concentration in primary knee OA patients; (p<0.001) and (p<0.05) in group Ι and group ΙΙ respectively as well as significant improvements in WOMAC total and WOMAC sub-scores values were noted after single intra-articular PRP injection with maximal functional improvements were achieved after 3 months (p<0.001). Mild cases experienced favorable results with no remarkable adverse reactions were observed. Conclusion:Reduction in specific OA biomarker S.Coll2-1 following intra-articular PRP injection emphasize that PRP could be a promising safe and tolerable effective therapeutic option which improves function from basal states in primary knee OA patients.

Osteoarthritis year in review 2018: biomarkers (biochemical markers)

“…The effect of one dose injection of intra-articular platelet-rich plasma (PRP) in the knee joint on a serum biomarker of cartilage degeneration, Coll2-1 was evaluated over a short period of 3 months. They reported a reduction in Coll2-1 following injection emphasizing that PRP could be a promising safe and tolerable effective therapeutic option 47 . In another study, clinical efficacy of TissueGene-C (TG-C), a cell and gene therapeutic consisting of non-transformed and retrovirally transduced chondrocytes to overexpress TGF-b1 (3:1), was evaluated for KOA patients.…”

Moving toward targeting the right phenotype with the right platelet-rich plasma (PRP) formulation for knee osteoarthritis

“…Other authors showed a reduction in cartilage catabolism after PRP, through serum peptide of the alpha-helical region of type II collagen. 63 Intra-articular PRP improved the appearance of inflamed synovium, and ultrasound changes (reduced vascularity and less effusion) correlated with clinical outcomes. 64 Calcitonin gene-related peptide expression mediates pain reduction and down-regulation of synovial inflammation induced by PRP.…”

Rosmarinic acid induces rabbit articular chondrocyte differentiation by decreases matrix metalloproteinase-13 and inflammation by upregulating cyclooxygenase-2 expression

“…OA is the most prevalent disease of joints in elderly patients, affecting many millions of individuals worldwide and resulting in knee pain and locomotor system disability [ 5 , 22 ]. OA is characterized by intra-articular inflammation, cartilage degeneration, and subchondral bone remodeling.…”

“…Particularly, this marker can be used as a factor to predict the response to therapy in OA. Collagen and proteoglycans are degradation and lost due to excessive secretion of MMPs and other proteolytic enzymes [ 22 , 28 , 29 ]. The MMP family contains approximately 28 members, which can be divided into subgroups such as collagenases, gelatinases, and stromelysins.…”