Decrease of serotonin receptor 2C in schizophrenia brains identified by high-resolution mRNA expression analysis

Castensson, Anja; Emilsson, Lina; Sundberg, Rolf; Jazin, Elena

doi:10.1016/s0006-3223(03)00526-2

Cited by 44 publications

(36 citation statements)

References 46 publications

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“…Using 55 SZ and 55 control prefrontal cortices (PFC), Castensson et al examined expression level of 16 candidate genes by real-time RT-PCR, and found that HTR2C was significantly downregulated in SZ. 28 We also reported significant downregulation of HTR2C in the PFC of patients with SZ and BD, by DNA microarray analysis of 60 brain samples (15 BD, 15 SZ, 15 MD as well as 15 controls). 29 Significant downregulation of HTR2C in PFC and amygdala was also reported in female suicide victims by RT-PCR.…”

Section: Htr2c and Mental Disorders: Mrna And Protein Expressionmentioning

confidence: 63%

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Serotonin receptor 2C and mental disorders: Genetic, expression, and RNA editing studies

Iwamoto¹,

Bundo²,

Kato³

2009

RNA Biology

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Section: Htr2c and Mental Disorders: Mrna And Protein Expressionmentioning

confidence: 63%

“…40,41 However, the decreased expression of HTR2C in patients may not be fully attributable to the effect of medication, since medication-free patients also showed decreased mRNA expressions in the above studies. 28,29 …”

Section: Htr2c and Mental Disorders: Mrna And Protein Expressionmentioning

confidence: 99%

Serotonin receptor 2C and mental disorders: Genetic, expression, and RNA editing studies

Iwamoto¹,

Bundo²,

Kato³

2009

RNA Biology

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“…In support of this theory, clozapine, which is a traditional anti-psychotic drug, is a serotonergic antagonist [73], and some new medications function as both serotonin and dopamine modulators [74]. As with dopamine, studies investigating a genetic link between serotonin genes and schizophrenia have produced both positive and negative findings [75][76][77], thus suggesting that variation of serotonin related genes may influence the disease.…”

Section: Neurotransmittersmentioning

confidence: 99%

Omega-3 fatty acids and neuropsychiatric disorders

2005

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-Epidemiological evidence suggests that dietary consumption of the long chain omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), commonly found in fish or fish oil, may modify the risk for certain neuropsychiatric disorders. As evidence, decreased blood levels of omega-3 fatty acids have been associated with several neuropsychiatric conditions, including Attention Deficit (Hyperactivity) Disorder, Alzheimer's Disease, Schizophrenia and Depression. Supplementation studies, using individual or combination omega-3 fatty acids, suggest the possibility for decreased symptoms associated with some of these conditions. Thus far, however, the benefits of supplementation, in terms of decreasing disease risk and/or aiding in symptom management, are not clear and more research is needed. The reasons for blood fatty acid alterations in these disorders are not known, nor are the potential mechanisms by which omega-3 fatty acids may function in normal neuronal activity and neuropsychiatric disease prevention and/or treatment. It is clear, however, that DHA is the predominant n-3 fatty acid found in the brain and that EPA plays an important role as an anti-inflammatory precursor. Both DHA and EPA can be linked with many aspects of neural function, including neurotransmission, membrane fluidity, ion channel and enzyme regulation and gene expression. This review summarizes the knowledge in terms of dietary omega-3 fatty acid intake and metabolism, as well as evidence pointing to potential mechanisms of omega-3 fatty acids in normal brain functioning, development of neuropsychiatric disorders and efficacy of omega-3 fatty acid supplementation in terms of symptom management.

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“…Quantification of specific mRNA from the cell at a certain stage is one of the most important characteristics for expressing physiological features [1][2][3]. Measuring the amount of a specific mRNA among thousands of different mRNAs is the most difficult part of the analysis due to their similar chemical properties except for the nucleotide sequences.…”

Section: Introductionmentioning

confidence: 99%

A precise mRNA quantification method using CE‐based SSCP

et al. 2006

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Even though mRNA quantification provides significant information for biological analysis, current methods such as Northern blot analysis and real-time PCR are known to be laborious and lacking in precision. In this study, we demonstrate a new precise mRNA quantification method using CE based on SSCP (CE-SSCP) coupled with reverse transcription. mRNA samples could be simply analyzed for the quantification directly with reverse transcript obtained from a single reaction. This helps to avoid considerable errors generated by a series of the tedious manual steps. Also, unlike real-time PCR, reverse transcripts can be directly quantified by CE-SSCP in this method without further data estimation. Reproducibility and accuracy of CE-SSCP for mRNA quantification was examined using enhanced green fluorescent protein (eGFP) mRNA transcribed in vitro. Specific reverse transcription primer was determined for the accurate quantification of eGFP mRNA from total RNA obtained from the recombinant Escherichia coli. Using elongation factor Tu mRNA as an internal standard, it was shown that sample-to-sample variation could be minimized. Expression kinetics at both mRNA level and protein level was studied and the potential of CE-SSCP in expression analysis was demonstrated by comparison with the eGFP activity assay.

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Decrease of serotonin receptor 2C in schizophrenia brains identified by high-resolution mRNA expression analysis

Cited by 44 publications

References 46 publications

Serotonin receptor 2C and mental disorders: Genetic, expression, and RNA editing studies

Serotonin receptor 2C and mental disorders: Genetic, expression, and RNA editing studies

Omega-3 fatty acids and neuropsychiatric disorders

A precise mRNA quantification method using CE‐based SSCP

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