Decrease in the regeneration potential of long-term cell suspension cultures of Lilium formosanum Wallace and its restoration by the auxin transport inhibitor, 2,3,5-triiodobenzoic acid

Nakano, Masaru; Sakakibara, Toshiaki; Suzuki, Sakae; Saito, Hiroyuki

doi:10.1016/s0168-9452(00)00313-7

Cited by 40 publications

(20 citation statements)

References 30 publications

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“…However, after maintenance for one to 1.5 years, they showed a decrease in regeneration potential. The reduction in the regeneration potential with increasing time of subculture had already been reported in lily suspension cells (Nakano et al 2000) and in other plants, such as wheat (Redway et al 1990). This could be due to physiological changes in the cells (Nakano et al 2000).…”

Section: Discussionmentioning

confidence: 53%

Regeneration and Agrobacterium-mediated transformation of multiple lily cultivars

Wang

Kronenburg

Menzel

et al. 2012

Plant Cell Tiss Organ Cult

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To pursue genetic improvement of lily, efficiency of both regeneration and transformation from callus cultures induced from different explants were evaluated in multiple cultivars. Thirty-five callus lines induced from filaments or styles and one control callus line derived from bulb scales of in total twenty lily cultivars representing Lilium longiflorum, Oriental 9 Trumpet and Longiflorum 9 Asiatic hybrids were maintained on a medium with 8.3 lM picloram (PIC). In this study, they were tested for their regeneration potential by transferring them onto a regeneration medium supplemented with 0.4 lM PIC and 0.044 lM 6-benzyladenine. Regeneration was obtained in all cultivars examined and the percentage varied from zero to 89 % in the 36 callus lines. Regeneration frequency was significantly influenced by the genotype (cultivar). Subculturing the calli every 4 weeks by refreshing the regeneration medium contributed positively to bulblet formation, when compared to an eight week subculture frequency. It was found that the regeneration ability generally decreased with an increasing age of the callus cultures for all cultivars. The origin of the callus (style or filament) did not lead to significant differences in regeneration frequency, but there was an interaction between callus origin and genotype. Calli of eight randomly chosen cultivars were co-cultivated with Agrobacterium tumefaciens strain AGL0 carrying binary vectors with the gus gene as reporter and putative transgenic plants were produced. GUS histochemical assays demonstrated transient and stable expression of the gus gene in both calli and regenerated lily plants. Transient expression frequencies ranged from 0.3 to 20.6 % while stable transformation was much lower, only 1.4 % as the maximum.

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Section: Discussionmentioning

confidence: 53%

Regeneration and Agrobacterium-mediated transformation of multiple lily cultivars

Wang

Kronenburg

Menzel

et al. 2012

Plant Cell Tiss Organ Cult

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“…Next, it requires the formation of the embryo proper, which is achieved upon transfer of the embryogenic cells to the hormone-free medium (here, lily regeneration through these procedures). A number of laboratories have already reported the regeneration of lily plants through organogenesis (Stimart and Ascher 1978;Liu and Burger 1986;Priyadarshi and Sen 1992;ArzateFernandez et al 1997;Nhut 1998), while regeneration of lilies through somatic embryogenesis has also been reported (Tribulato et al 1997;Nakano et al 2000). Tribulato et al (1997) confirmed the bipolarity of regenerants by histological investigation of the differentiation process in Lilium longiflorum.…”

Section: Plant Regenerationmentioning

confidence: 85%

Regeneration of flowering plants from difficile lily protoplasts by means of a nurse culture

Horita¹,

Morohashi²,

Komai³

2002

Planta

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The regeneration of difficile lily protoplasts isolated from suspension cells of the Oriental hybrid lily (Lilium L.) cultivars Casablanca, Siberia and Acapulco was achieved by using the nurse-culture method. The divided protoplasts grew into colonies with nurse cells that have no regeneration ability, and developed to visible calli on a medium containing picloram. Many plantlets were formed on the calli after transfer of the proliferated calli to hormone-free medium. We were able to transplant the plantlets to soil in pots without acclimatization, and the plantlets grew in a greenhouse until flowering 2 years later.

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“…Callus formation and plant regeneration from induced callus were observed in 33 Lilium genotypes (11). Most successful reports of in vitro regeneration of Lilium have focused on L. longiflorum genotypes and other popular Lilium species (10,13,14,15,16,17). Researches on the regeneration of wild lilies have been seldom reported (4,23).…”

Section: Introductionmentioning

confidence: 99%

Callus Induction and Plant Regeneration fromIn VitroCultured Leaves, Petioles and Scales ofLilium Leucanthum(Baker) Baker

Tang

Liu

Gituru

et al. 2010

Biotechnology & Biotechnological Equipment

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Decrease in the regeneration potential of long-term cell suspension cultures of Lilium formosanum Wallace and its restoration by the auxin transport inhibitor, 2,3,5-triiodobenzoic acid

Cited by 40 publications

References 30 publications

Regeneration and Agrobacterium-mediated transformation of multiple lily cultivars

Regeneration and Agrobacterium-mediated transformation of multiple lily cultivars

Regeneration of flowering plants from difficile lily protoplasts by means of a nurse culture

Callus Induction and Plant Regeneration fromIn VitroCultured Leaves, Petioles and Scales ofLilium Leucanthum(Baker) Baker

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