Decrease in lung nitric oxide production after peritonitis in mice with sickle cell disease*

Bartolucci, Pablo; Ngo, Minh-Triet; Beuzard, Yves; Galactéros, Frédéric; Saber, Guitanouch; Rideau, Dominique; Eddahibi, Saadia; Maître, Bernard; Adnot, Serge; Delclaux, Christophe

doi:10.1097/01.ccm.0000253403.65602.ea

Cited by 9 publications

(4 citation statements)

References 44 publications

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“…There is no definite explanation for the decreased NO production; we previously suggested that it could result from an increased arginase activity, which uses the same precursor, L-arginine (9,16). Deja and colleagues (27) showed that in critically ill patients with sinusitis and sepsis, maxillary NO production is almost completely suppressed by downregulation of iNOS mRNA.…”

Section: Discussionmentioning

confidence: 95%

Prediction of Nosocomial Infection Acquisition in Ventilated Patients by Nasal Nitric Oxide

Tadié

Trinquart²,

Jannière-Nartey³

et al. 2010

Shock

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The development of biomarkers able to predict the occurrence of nosocomial infection could help manage preventive strategies, especially in medical patients whose degree of acquired immunosuppression may be variable. We hypothesized that the NO fraction present in the airways (upper and lower) of critically ill patients under mechanical ventilation could constitute such a biomarker. We conducted an observational proof-of-concept study in a medical intensive care unit of a teaching hospital. Forty-five patients (26 men; 72 [25th-75th percentiles] years [56-82]; Simplified Acute Physiology Score II, 63 [50-81], 14 infected) under mechanical ventilation (>3 days) underwent on day 1 and day 3 of their stay: nasal and exhaled (partitioned in bronchial and alveolar sources) bedside NO measurements, determination of urine NO end products and plasma cytokine (IL-6, IL-10) concentrations, and Sequential Organ Failure Assessment score calculation. Nosocomial infection incidence was recorded during the 15 subsequent days. Fifteen patients (33%) acquired a nosocomial infection (16 infections, 15 ventilator-associated pneumonia and 1 bacteremia). Nasal NO was the only marker significantly different between patients with and without subsequent infection (day 1, 52 ppb [20-142] vs. 134 [84-203], P = 0.038; day 3, 98 ppb [22-140] vs. 225 [89-288], P = 0.006, respectively). Nasal NO fraction 148 ppb or less at day 3 had an 80% sensitivity, a 70% specificity, and an odds ratio of 2.7 (95% confidence interval, 1.9-3.8) to predict acquisition of nosocomial infection. Nonsurvivors had a higher IL-6 concentration on day 3 (P = 0.014), whereas their nasal NO fractions were not significantly different. Nasal NO seems to be a relatively sensitive and specific biomarker of subsequent nosocomial infection acquisition (at least for ventilator-associated pneumonia), which warrants confirmation in a multicenter trial.

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Section: Discussionmentioning

confidence: 95%

Prediction of Nosocomial Infection Acquisition in Ventilated Patients by Nasal Nitric Oxide

Tadié

Trinquart²,

Jannière-Nartey³

et al. 2010

Shock

Self Cite

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“…One hypothesis for this deleterious effect is that hypoxia‐induced RBC haemolysis in mice that received normal RBCs decreased NO bioavailability. As NO modulates the expression of endothelin receptors, a decrease of NO during a SCD crisis could have upregulated EDN1 expression (Reiter & Gladwin, ; Bartolucci et al , ; Gladwin & Kato, ; Wood et al , ). The benefit of IHP‐RBCs over normal RBCs in BERK mice was also observed on inflammatory processes.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of homologous inositol hexaphosphate‐loaded red blood cells in sickle transgenic mice

et al. 2012

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SummaryPatients with sickle cell disease (SCD) can present several severe symptoms during their lifetime, including painful events due to vascular occlusion (VOC). Even though multiple factors are involved in VOC, hypoxia is the most important triggering factor. Inositol hexaphosphate (IHP) reduces the oxygen-haemoglobin affinity thus improving the oxygen release in the blood stream and in the tissues. Thus, IHP-loaded homologous red blood cells (IHP-RBCs) could be able to reduce disorders in SCD. The effectiveness of treatment was assessed in two types of SCD transgenic mice (BERK and SAD). The administration of four repeated injections of IHP-RBCs in BERK mice resulted in an improved survival rate and brain development, prevention of severe anaemia and a greatly lowered risk of VOC. After one injection of IHP-RBCs, SAD mice were subjected to acute hypoxic stress. Analysis of the lungs revealed significantly decreased mRNA levels of molecules involved in intravascular disorders. Our results showed that transfusion of homologous IHP-RBCs, by increasing the oxygen delivery, reduces SCD disorders in sickle transgenic mice.

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“…In animal models, exhaled NO can also be used to indirectly assess airway hyperresponsiveness [51,56,66], or to evaluate other types of lung disorders, e.g. acute lung injury (ALI) [40,52,68], pulmonary vascular diseases [60,61,64], and distal lung inflammation [35,36,38,57,63,65,74]. Repeated exhaled NO measurement in chronic disease models is now possible showing us a concept about using exhaled NO as a predictor for late complication in humans.…”

Section: Discussionmentioning

confidence: 99%

“…Exhaled NO can also be measured non-invasively in awake animal by using a closed chamber, although the mixed exhaled NO is not specific for NO production in lower airway [62]. This method has been successfully applied in both rats [36,43,63,64] and mice [33,51,56,57,62,65]. The closed chamber is connected to the ventilator on one side and the analyzer of NO on the other.…”

Section: Two Approaches To Choose: Invasive or Non-invasivementioning

confidence: 99%

Measuring exhaled nitric oxide in animal models: methods and clinical implications

Le-Dong¹,

Duong‐Quy²,

Bei³

et al. 2012

J. Breath Res.

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Animal models such as rats and mice are useful for studying the multiple roles of nitric oxide (NO) in various respiratory disorders. The production of NO is catalyzed by the three isoforms of the enzymes (NO synthases; NOS). Indirect assessment of NOS gene or protein expression only provides partial information on the role of NO in health and lung disease. NO can also be measured in exhaled air by invasive or non-invasive approaches as a direct and quantitative marker of NO production in animal models. Whilst addressing the different methods of exhaled NO analysis in small animals (rats and mice), this review also focuses on the possible clinical implications, and discusses the advantages and limitations of these methods.

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Decrease in lung nitric oxide production after peritonitis in mice with sickle cell disease*

Abstract: Acute peritonitis in SAD mice is associated with a defect in lung nitric oxide production and bioavailability that may participate in the acute systemic and lung vaso-occlusive complications of sickle cell disease.

Cited by 9 publications

References 44 publications

Prediction of Nosocomial Infection Acquisition in Ventilated Patients by Nasal Nitric Oxide

Prediction of Nosocomial Infection Acquisition in Ventilated Patients by Nasal Nitric Oxide

Efficacy of homologous inositol hexaphosphate‐loaded red blood cells in sickle transgenic mice

Measuring exhaled nitric oxide in animal models: methods and clinical implications

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