Decrease in Luminescence Lifetime Indicating Nonradiative Energy Transfer from Upconverting Phosphors to Fluorescent Acceptors in Aqueous Suspensions

Riuttamäki, Terhi; Hyppänen, Iko; Kankare, Jouko; Soukka, Tero

doi:10.1021/jp2056915

Cited by 37 publications

(43 citation statements)

References 35 publications

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“…Examples are sol-gel [147,152,154,249], ethyl cellulose or PVC membranes [61], different types of ORMOSILs [39,94,106], polystyrene [91], polysulfones [11,18], poly(dimethylsiloxane) alone [251] or with different amounts of pendant acrylate groups [168], and a blended fluoropolymer matrix consisting of Nafion ® and Aflas ® [83]. In another approach, the [Ru(dpp) 3 ] 2+ complex was adsorbed onto silica and then dispersed in a silicone rubber support [38,93].…”

Section: Transition Metal Polypyridyl Complexesmentioning

confidence: 99%

“…The key mechanisms that are recognized as supporting microbial EET both to and from electrodes, or are proposed as doing so, are discussed in later sections. [167][168][169][170]. In addition, the involvement of more than one pathway, or overlapping pathways, in mineral respiration has been suggested, for instance in the case of Shewanella sp.…”

Section: Microorganisms Exploited In Bioelectrochemical Systemsmentioning

confidence: 99%

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Advances in Chemical Bioanalysis

Matysik¹

2014

Bioanalytical Reviews

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Bioanalytical Reviews is the successor of the former review journal with the same name, and it will complement Springer's successful and reputed review book series program in the flourishing and exciting area of the Bioanalytical Sciences.Bioanalytical Reviews (BAR) publishes reviews covering all aspects of bioanalytical sciences. It therefore is a unique source of quick and authoritative information for anybody using bioanalytical methods in areas such as medicine, biology, biochemistry, genetics, pharmacology, biotechnology, and the like.Reviews of methods include all modern tools applied, including chromatography (in its various forms), capillary electrophoresis, biosensors, bioelectroanalysis, fluorescence, mass spectrometry, NMR, IR/Raman, and other optical spectroscopies as well as methods related to bioimaging. In particular the series volumes provide reviews on perspective new instrumental approaches as they apply to bioanalysis, and on the use of micro-/nano-materials such as micro-and nanoparticles. Articles on μ-total analytical systems (μ-TAS) and on labs-on-a-chip also fall into this category.In terms of applications, reviews on novel bioanalytical methods based on the use of enzymes, DNAzymes, antibodies, cell slices, to mention the more typical ones, are highly welcome. Articles on subjects related to the areas including genomics, proteomics, metabolomics, high-throughput screening, but also bioinformatics and statistics as they relate to bioanalytical methods are of course also welcome. Reviews cover both fundamental aspects and practical applications.Reviews published in BAR are (a) of wider scope and authoratively written (rather than a record of the research of single authors), (b) critical, but balanced and unbiased; (c) timely, with the latest references. BAR does not publish (a) reviews describing established methods of bioanalysis; (b) reviews that lack wider scope, (c) reviews of mainly theoretical nature.

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Section: Transition Metal Polypyridyl Complexesmentioning

confidence: 99%

Section: Microorganisms Exploited In Bioelectrochemical Systemsmentioning

confidence: 99%

Advances in Chemical Bioanalysis

Matysik¹

2014

Bioanalytical Reviews

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“…The main merit of the UCP 168 T. Riuttamäki (née Rantanen) and T. Soukka label technology based on anti-Stokes photoluminescence detection is the absence of autofluorescence, which commonly deteriorates the detection limits of assays involving biological components. The autofluorescence commonly originates from endogenous fluorophores of biological material and is thus observed at wavelengths longer than the excitation radiation-equally to the fluorescence of conventional labels.…”

Section: Excitation With Near-infrared Radiationmentioning

confidence: 99%

“…Instead, they increase the background emission mainly originating from the unbound acceptors reabsorbing the UCP emission through a photon-mediated way, which is especially prominent in the case of UCP particles having a relatively large volume compared to the surface area. Our research group concluded based on luminescence lifetime data that both nonradiative UC-RET and photon reabsorption were present in a system involving UCP donor with average diameter around 110 nm [168]. As the quantity of the photon reabsorption is concentration dependent, it is possible to maximize the signal-to-background ratio of the homogeneous RET-based assay by carefully optimizing the donor and acceptor concentrations.…”

Section: Upconversion Resonance Energy Transfermentioning

confidence: 99%

Upconverting Phosphor Labels for Bioanalytical Assays

Riuttamäki

Soukka

2013

Advances in Chemical Bioanalysis

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Upconverting phosphors (UCPs) are photoluminescent inorganic crystals capable of photon upconversion upon absorption of two or more sequential photons. UCPs produce bright, structured emission at wavelengths shorter than the excitation radiation rendering them highly advanced labels for bioanalytical assays. The exceptional anti-Stokes emission allows total elimination of autofluorescence originating mainly from biomolecules and other sample components. UCPs are excited with near-infrared radiation which provides several advantages over ultraviolet excitation-including optimal penetration of biological material. This chapter summarizes the bioanalytical in vitro applications of UCPs and discusses the advantages and challenges of the emerging label technology. In addition, synthesis of the UCP labels and some of the instruments used for UCP detection are shortly reviewed. Bioimaging and therapeutic applications based on UCPs are outside of the scope of this chapter.

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“…This has advantages in some applications, particularly those that sense changes in the local environment by measuring changes in the luminescence lifetime. [11][12][13][14][15] However, this long lifetime is a hindrance when imaging with a fast scanning microscopy technique, with a pixel dwell time as short as 1 μs. For a pixel dwell time less than 500 μs, nanoparticle emission is streaked along the fast-scan axis of the microscope (Fig.…”

Section: Introductionmentioning

confidence: 99%

Scanning two-photon microscopy with upconverting lanthanide nanoparticles via Richardson-Lucy deconvolution

2012

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Abstract. The use of upconverting lanthanide nanoparticles in fast-scanning microscopy is hindered by a long luminescence decay time, which greatly blurs images acquired in a nondescanned mode. We demonstrate herein an image processing method based on Richardson-Lucy deconvolution that mitigates the detrimental effects of their luminescence lifetime. This technique generates images with lateral resolution on par with the system's performance, ∼1.2 μm, while maintaining an axial resolution of 5 μm or better at a scan rate comparable with traditional two-photon microscopy. Remarkably, this can be accomplished with near infrared excitation power densities of 850 W∕cm 2 , several orders of magnitude below those used in two-photon imaging with molecular fluorophores. By way of illustration, we introduce the use of lipids to coat and functionalize these nanoparticles, rendering them water dispersible and readily conjugated to biologically relevant ligands, in this case epidermal growth factor receptor antibody. This deconvolution technique combined with the functionalized nanoparticles will enable threedimensional functional tissue imaging at exceptionally low excitation power densities.

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Decrease in Luminescence Lifetime Indicating Nonradiative Energy Transfer from Upconverting Phosphors to Fluorescent Acceptors in Aqueous Suspensions

Cited by 37 publications

References 35 publications

Advances in Chemical Bioanalysis

Advances in Chemical Bioanalysis

Upconverting Phosphor Labels for Bioanalytical Assays

Scanning two-photon microscopy with upconverting lanthanide nanoparticles via Richardson-Lucy deconvolution

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