Decrease in Glycogen Phosphorylation in Muscles In vitro after Adrenalectomy and Restoration with Desoxycorticosterone

Phosphorolysis and synthesis of glycogen in animal tissues

“…The results agree with those of Holmes & Lehmann [1940] who, working on liver, found no loss of phosphorolysis activity in minced liver of adrenalectomized rabbits. They contradict those of Verzar & Montigel'[1942], who found that phosphorolysis in the muscles of adrenalectomized rats was almost completely abolished when short incubation times (up to 30 min.) were used.…”

Chemical Changes in the Tissues of the Rat After Adrenalectomy

“…The fall of the idiomolar value in the three tissues is of interest in view of the changes in hexose phosphate and glycogen content observed in two of those tissues [Verzàr & Montigiel, 1942], and these may account in part for the decrease in the nondiffusible molecules in the cells. The part played by the non-diffusible phosphate esters in the increase of the electrostatic equivalent has been discussed by Conway and his associates [Boyle & Conway, 1941;Conway, 1945;Conway & Hingerty, 1946], whose findings in skeletal muscle from adrenalectomized rats have been confirmed.…”

The influence of adrenalectomy on muscle constituents

“…Tissue culture technique (a) General. Cultures were grown by the roller tube technique of Willmer (1942) which we have previously employed (Davidson & Waymouth, 1943, 1945. Each roller tube contained 12 pieces of tissue in two rows of 6.…”