Decoupled control of stiffness and permeability with a cell-encapsulating poly(ethylene glycol) dimethacrylate hydrogel

Calcium-Alginate Hydrogel-Encapsulated Fibroblasts Provide Sustained Release of Vascular Endothelial Growth Factor

“…50 Swelling of the Ca-alginate hydrogel results from the release of calcium crosslinks into the culture medium, 21 and is associated with an increase in pore size. 50,51 This allows increased diffusion 52 of nutrients and waste products to occur with scaffold degradation to maintain viability of increasing cell numbers that are likely to occur due to migration of cells into the scaffold after implantation. The relatively slow degradation of the scaffold, compared with materials such as collagen, will also ensure that an area for CMs and ECs to invade is maintained over longer time period, while retaining implanted fibroblasts at the desired implantation site to generate prolonged localized VEGF release.…”

Functional nucleus pulposus-like matrix assembly by human mesenchymal stromal cells is directed by macromer concentration in photocrosslinked carboxymethylcellulose hydrogels

“…Acellular hydrogel characterization provided insight into the key physical properties influenced by CMC macromer concentration. The 1.5 % acellular gels had a higher baseline swelling ratio compared with the 3.5 % gels, a property that is indicative of increased water content and hydrogel porosity (Cha et al 2010). The H A of the 3.5 % acellular hydrogels was significantly greater than that for both of the lower macromer concentrations, indicating that the polymer network is stiffer and more densely crosslinked.…”

Integrative design of a poly(ethylene glycol)-poly(propylene glycol)-alginate hydrogel to control three dimensional biomineralization

“…The charge density of a hydrogel was calculated from the amount of free carboxylic groups in MA, which was measured using titration [30]. Briefly, 300 mL of 1 wt% MA was diluted with DI water to 5 mL.…”