Deconvolution of Multiexponential Fluorescence Spectra with MIM

Maximum Entropy Method for Frequency Domain Fluorescence Lifetime Analysis. 1. Effects of Frequency Range and Random Noise

“…Improvements in instrumentation have improved precision, accuracy, and dynamic range, allowing for analysis of complicated fluorescence decays of fluorophores in complex systems such as organized media and biological samples. , Unfortunately, the use of standard data analysis techniques to resolve these decays is often inadequate. This has led to the evolution of new data analysis strategies to improve the accuracy and reliability of the lifetime information extracted from the data. − One analysis method that has shown significant promise for use with both time and frequency domain lifetime data is the maximum entropy method (MEM). 3,4,9-12 However, the use of MEM is still somewhat experimental, and many questions persist concerning its use.…”