Decondensation of cationic gemini surfactant-induced DNA aggregates using triblock copolymer (PEO)20–(PPO)70–(PEO)20

He, Yurong; Xu, Shouhong; Sun, Di; Shang, Yazhuo; Zhao, Xiaohong; Liu, Honglai

doi:10.1007/s00396-013-2954-5

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…According to Y. He et al 51 and Z. Xiaofang et al 52 these changes are consistent with the formation of a chiral C-phase corresponding to tightly packed structures of DNA with the 12-3-12.2Br surfactants at the Paper PCCP outside layers. This assumption is supported from the AFM images obtained, where surfactant aggregates appeared bound to DNA condensed structures (see Fig.…”

Section: Discussionsupporting

confidence: 60%

Improving the understanding of DNA–propanediyl-1,3-bis(dodecyldimethylammonium) dibromide interaction using thermodynamic, structural and kinetic approaches

Grueso

Kuliszewska

Prado‐Gotor

et al. 2013

Phys. Chem. Chem. Phys.

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A kinetic, thermodynamic and structural study of the interaction of the gemini surfactant propanediyl-1,3-bis(dimethyldodecylammonium dibromide) (12-3-12.2Br) with calf thymus DNA was carried out at several ionic strengths (NaCl) in aqueous solutions. A new 12-3-12(2+)-selective membrane was prepared in order to gain insight into the factors that control the binding of 12-3-12.2Br to DNA. We used ethidium bromide (EB) as a fluorescence probe to follow the kinetics of the interaction by using the stopped-flow fluorescence technique. The results can be explained in terms of a reaction mechanism involving two consecutive reversible (fast and slow) steps. The fast step was attributed to the union/separation of the surfactant with/from the DNA polynucleotide. Changes in the kinetic constants in the forward and backward directions were discussed in terms of the Brönsted-Pitzer equation and of the increase in hydrophobic interactions of the surfactant tails as a consequence of salting-out effects, respectively. The slow step corresponds to a conformational change of the surfactant-DNA complex to a more compacted form. The equilibrium constant, calculated from the forward and reverse rate constants of these steps, agrees with the results obtained from potentiometric titration using a 12-3-12-(2+) selective electrode.

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Section: Discussionsupporting

confidence: 60%

Improving the understanding of DNA–propanediyl-1,3-bis(dodecyldimethylammonium) dibromide interaction using thermodynamic, structural and kinetic approaches

Grueso

Kuliszewska

Prado‐Gotor

et al. 2013

Phys. Chem. Chem. Phys.

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“…The bound surfactant molecules may then form micelle-like aggregates through hydrophobic interactions between their hydrocarbon tails, creating nucleation centers that DNA can wrap around to form a densely packed, or “bead-like,” structure . This compaction can be reversed to dissociate the DNA molecule from the surfactant (whether mono- or dicationic) using a decrease in temperature or pH or the addition of various reagents such as sodium dodecyl sulfate , or other anionic surfactants, cyclodextrins, ,, nonionic surfactants, monovalent salts, synthetic polyacids, and nucleotides . Depending on the reagent used, varying levels of decompaction and different final DNA conformations are obtained …”

Section: Introductionmentioning

confidence: 99%

Cationic Gemini Surfactant–Plasmid Deoxyribonucleic Acid Condensates as a Single Amphiphilic Entity

Shortall

Wettig

2017

J. Phys. Chem. B

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A critical aggregate concentration for the surfactant-DNA "complex" or "condensate" consisting of the 16-3-16 gemini surfactant and circular plasmid DNA was determined using surface tensiometry, dynamic light scattering, and conductometry. This surfactant-DNA complex acts as an amphiphile itself, for example, decreasing the surface tension of water until a critical concentration is reached. The evidence presented here introduces a new way of considering these surfactant-DNA condensates, not simply as aggregates in solution but as surface-active agents in their own right. At concentrations below the critical aggregate concentration, there is some dissociation of surfactant molecules from the condensate, creating a more "loose" or "relaxed" complex; however, at and above the critical aggregate concentration, the surfactant-DNA system forms smaller and more uniformly distributed condensates once again. This behavior is analogous to demicellization/micellization that occurs in typical surfactant systems.

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“…[28] He et al successfully achieved the decondensation of DNA molecules by introducing triblock copolymer (PEO) 20 -(PPO) 70 -(PEO) 20 (P123), and it is found that the release of the surfactant from the complex induced by P123 turns DNA conformation from ψ-phase back to Bform. [29] It is noticed that multivalent cations play an important role in controlling the morphologies of DNA chains in solutions. Muthukumar et al have determined the electrostatic interactions screening in a salt solution based on the double screening theory for multi-polyelectrolytes.…”

Section: Introductionmentioning

confidence: 99%

Decondensation behavior of DNA chains induced by multivalent cations at high salt concentrations: Molecular dynamics simulations and experiments

Jiang

Ran

et al. 2015

Chinese Phys. B

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Using molecular dynamics simulations and atomic force microscopy (AFM), we study the decondensation process of DNA chains induced by multivalent cations at high salt concentrations in the presence of short cationic chains in solutions. The typical simulation conformations of DNA chains with varying salt concentrations for multivalent cations imply that the concentration of salt cations and the valence of multivalent cations have a strong influence on the process of DNA decondensation. The DNA chains are condensed in the absence of salt or at low salt concentrations, and the compacted conformations of DNA chains become loose when a number of cations and anions are added into the solution. It is explicitly demonstrated that cations can overcompensate the bare charge of the DNA chains and weaken the attraction interactions between the DNA chains and short cationic chains at high salt concentrations. The condensation-decondensation transitions of DNA are also experimentally observed in mixing spermidine with λ-phage DNA at different concentrations of NaCl/MgCl2 solutions.

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Decondensation of cationic gemini surfactant-induced DNA aggregates using triblock copolymer (PEO)20–(PPO)70–(PEO)20

Cited by 5 publications

References 31 publications

Improving the understanding of DNA–propanediyl-1,3-bis(dodecyldimethylammonium) dibromide interaction using thermodynamic, structural and kinetic approaches

Improving the understanding of DNA–propanediyl-1,3-bis(dodecyldimethylammonium) dibromide interaction using thermodynamic, structural and kinetic approaches

Cationic Gemini Surfactant–Plasmid Deoxyribonucleic Acid Condensates as a Single Amphiphilic Entity

Decondensation behavior of DNA chains induced by multivalent cations at high salt concentrations: Molecular dynamics simulations and experiments

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