Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

Yoshida, Kentaro; Kashimura, Yu; Kamijo, Toshio; Ono, Tetsuya; Dairaku, Takenori; Sato, Takaya; Kashiwagi, Yoshitomo; Sato, Katsuhiko

doi:10.3390/polym12020319

Cited by 7 publications

(3 citation statements)

References 44 publications

(72 reference statements)

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“…This design was later developed into a glucose-sensitive nanofilm, composed of five bilayers of LBL-assembled hemin-appended poly(ethyleneimine) (H-PEI) and glucose oxidase-loaded DNA [ 36 ]. When glucose oxidase reacts with glucose, gluconic acid and H 2 O 2 are produced, and H 2 O 2 further reacts with hemin to generate ROS which cleave DNA and promote the nanofilm degradation.…”

Section: Dna Based Drug Delivery Vehiclesmentioning

confidence: 99%

Sequence Does Not Matter: The Biomedical Applications of DNA-Based Coatings and Cores

Batasheva

Fakhrullin

2021

IJMS

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Biomedical applications of DNA are diverse but are usually associated with specific recognition of target nucleotide sequences or proteins and with gene delivery for therapeutic or biotechnological purposes. However, other aspects of DNA functionalities, like its nontoxicity, biodegradability, polyelectrolyte nature, stability, thermo-responsivity and charge transfer ability that are rather independent of its sequence, have recently become highly appreciated in material science and biomedicine. Whereas the latest achievements in structural DNA nanotechnology associated with DNA sequence recognition and Watson–Crick base pairing between complementary nucleotides are regularly reviewed, the recent uses of DNA as a raw material in biomedicine have not been summarized. This review paper describes the main biomedical applications of DNA that do not involve any synthesis or extraction of oligo- or polynucleotides with specified sequences. These sequence-independent applications currently include some types of drug delivery systems, biocompatible coatings, fire retardant and antimicrobial coatings and biosensors. The reinforcement of DNA properties by DNA complexation with nanoparticles is also described as a field of further research.

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Section: Dna Based Drug Delivery Vehiclesmentioning

confidence: 99%

Sequence Does Not Matter: The Biomedical Applications of DNA-Based Coatings and Cores

Batasheva

Fakhrullin

2021

IJMS

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“…A large number of methods have been proposed to immobilize enzymes on electrode surfaces, concomitantly preserving enzymatic activity and designing efficient electrontransfer pathways between the immobilized enzyme and electrode surface, preventing unspecific side reactions [11][12][13]. Among them, the mostly commonly applied are selfassembled monolayers [14], Langmuir-Blodgett films [15], sol-gel methods [16,17], and LbL films [18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Layer-By-Layer Films of Silsesquioxane and Nickel(II) Tetrasulphophthalocyanine as Glucose Oxidase Platform Immobilization: Amperometric Determination of Glucose in Kombucha Beverages

et al. 2023

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This paper describes the development of a novel glucose biosensor through the layer-by-layer technique (LbL). The self-assembled architectures were composed of a positive-charged silsesquioxane polyelectrolyte, 3-n-propylpyridinium silsesquioxane chloride (SiPy+Cl−), nickel (II) tetrassulphophthalocyanine (NiTsPc), and a conductive surface of FTO (fluor tin oxide). The construction of the biosensor was influenced by the isoelectric point (pI) of the glucose oxidase enzyme (GOx), which allowed electrostatic interaction between the outer layer of the silsesquioxane film and the enzyme. The architecture of modified electrode GOx/(SiPy+Cl−/NiTsPc)5.5/FTO was confirmed by UV-Vis, FTIR, and chronoamperometry techniques using different immobilization methods of GOx. Among the studied methods, a higher variation of current was observed for the modified electrode formed by mixed LbL films of SiPy+Cl− and NiTsPc and the enzyme immobilized by drop coating. The stability and reproducibility of the biosensor were verified when the last layer containing the enzyme was coated with 0.2% Nafion® polymer. Under these conditions, a linear response for glucose was obtained in the concentration range of 0.2 to 1.6 mmol L−1 (R2 = 0.991) with a limit of detection of 0.022 mmol L−1. The proposed biosensor was applied to quantify glucose in two different samples of kombucha juices with accuracy, allowing the glucose content of the healthy beverages to be estimated.

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“…Such LbL films are prepared using various polymers for the development of thin film devices. Examples of the polymer materials used include synthetic polymers [10,11], polysaccharides [12][13][14], proteins [15,16], and DNA [17][18][19]. Thin films with various functions can be developed using polymers that are suitable for the intended application.…”

Section: Introductionmentioning

confidence: 99%

Adsorption and Release of Rose Bengal on Layer-by-Layer Films of Poly(Vinyl Alcohol) and Poly(Amidoamine) Dendrimers Bearing 4-Carboxyphenylboronic Acid

et al. 2020

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Phenylboronic acid-bearing polyamidoamine dendrimer (PBA-PAMAM)/poly(vinyl alcohol) (PVA) multilayer films were prepared through the layer-by-layer (LbL) deposition of PBA-PAMAM solution and PVA solution. PBA-PAMAM/PVA films were constructed successfully through the formation of boronate ester bonds between the boronic acid moiety in PBA and 1,3-diol units in PVA. When the (PBA-PAMAM/PVA)5 films were immersed in rose bengal (RB) solution, RB was adsorbed onto the LbL films. The amount of RB adsorbed was higher in the LbL films immersed in acidic solution than in basic solution. The release of RB from the LbL films was also promoted in the basic solution, while it was suppressed in the acidic solution. The boronic acid ester is oxidized to phenol by hydrogen peroxide (H2O2) and the carbon-boron bond is cleaved, so that the (PBA-PAMAM/PVA)5 films can be decomposed by immersion in H2O2 solution. Therefore, when RB-adsorbed (PBA-PAMAM/PVA)5 films were immersed in H2O2 solution, the release of RB was moderately promoted when the solution was weakly acidic.

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Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase

Cited by 7 publications

References 44 publications

Sequence Does Not Matter: The Biomedical Applications of DNA-Based Coatings and Cores

Sequence Does Not Matter: The Biomedical Applications of DNA-Based Coatings and Cores

Layer-By-Layer Films of Silsesquioxane and Nickel(II) Tetrasulphophthalocyanine as Glucose Oxidase Platform Immobilization: Amperometric Determination of Glucose in Kombucha Beverages

Adsorption and Release of Rose Bengal on Layer-by-Layer Films of Poly(Vinyl Alcohol) and Poly(Amidoamine) Dendrimers Bearing 4-Carboxyphenylboronic Acid

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