Decomposition and mineralization of aquatic humic substances (AHS) in treating landfill leachate using the Anammox process

Abstract

a b s t r a c tAquatic humic substance (AHS) in landfill leachate is resistant to biodegradation, especially in anaerobic habitats. However, we reported here for the first time that AHS was completely biodegraded with the anaerobic ammonium oxidation (Anammox) process. As a result of aromatic-ring cleavage and mineralization, carboxylic and aliphatic organics were significantly produced, the contents of chromophores such as quinoid and ketone were remarkably decreased to decolorize the leachate, and produced carbon dioxide along with increasing carbonate was clearly presented. Of the degraded AHS of 137 mg L À1 , 51 mg L À1 was owed to the oxidation with sulfate of 76 mg L À1 as electron acceptor, and the rest to other metabolic mechanism. Isolation and identification of heterotrophic bacteria revealed a diversified consortium comprising four facultative anaerobic species, Bacillus sp., Paenibacillus sp., Bacteroides sp. and Staphylococcus sp., without sulfate-reducing bacteria detected. Their contribution to AHS biodegradation and sulfate reduction under the special conditions with high oxidization-reduction potential and insufficient electron acceptors has not been known yet. Further work is underway to investigate their properties and respective duties in the consortium.

Sulfate-reducing anaerobic ammonium oxidation as a potential treatment method for high nitrogen-content wastewater

“…However, there are reports on other heterotrophic bacterial strains, except for SRB, reducing SO 4 2-as the terminal electron acceptor while utilizing organic substrates in a similar manner as SRB under anaerobic conditions (Liang et al 2009). Four facultative anaerobic species, Bacillus sp., Paenibacillus sp., Bacteroides sp.…”

“…The ''conventional'' anammox, however, is not sustainable in a medium where a high concentration of easily biodegradable organic matter is present (Chamchoi et al 2008) as denitrifying bacteria outcompete the anammox organisms. Anammox process has still been successfully applied for treatment of landfill leachate (Liang et al 2009) containing organic matter of more refractory nature.…”

Adsorption Behavior and Mechanism of Humic Acid on Aminated Magnetic Nanoadsorbent

“…Numerous methods have been used to reduce the HA in drinking water, such as membrane separation, ultra-filtration, chemical coagulation, ion exchange, adsorption, biodegradation, and so on (5)(6)(7)(8)(9)(10). Among these methods, adsorption method appears to be one of the most promising alternatives because of its simplicity of design, low-cost, and ease of operation.…”

Short-Term and Long-Term Inhibitory Effects of Copper on Anammox Process

“…The consistency of the inhibition model to the experimental data was fitted with minimum squared errors method and it was found that modified non-competitive is the most representative model. Also, some researchers used the modified non-competitive model to express the heavy metal inhibition on the biochemical process [27,34,36,37]. Therefore, IC50 values for short-term inhibition was determined by modified non-competitive inhibition model.…”