Decolourization of olive mill waste-waters by the white-rot fungus Phanerochaete chrysosporium: involvement of the lignin-degrading system

Sayadi, Sami; Ellouz, Radhouane

doi:10.1007/bf00174851

Cited by 102 publications

(74 citation statements)

References 25 publications

(16 reference statements)

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“…Aissam et al [13] argued that the degradation of phenolic compounds of olive mill wastewater using Aspergillus niger HA37was due to the possible action of various enzymes, including those of laccase, tannase and the ligninolytic oxidative enzymes, and demonstrated that the growth of Aspergillus niger in diluted olive mill wastewater resulted in a 70% degradation of the phenolic compounds. However, tannase probably was the principal enzymatic system of color removal because Aspergillus niger had a low LiP activity and Sayadi and Ellouz [5] have demonstrated that low LiP activity did not efficiently decolorized OMW.…”

Section: Study Of Enzymes Responsible For Topw Decolorizationmentioning

confidence: 99%

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Optimization of Biological Pretreatment of Green Table Olive Processing Wastewaters Using Aspergillus niger

Ayed¹,

Chammam²

2014

J Bioremed Biodeg

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Aspergillus niger has been tested in a discontinuous process for the pretreatment of fresh green Table Olive Processing Waste waters (TOPW). The influence of seven factors like incubation time, initial pH, dilution, glucose, agitation, (NH 4 ) 2 SO 4 and inoculum size on decolorization was screened by employing a fractional factorial design 2 7-4 . Through factorial plots and analysis of variance, it was found that only glucose and agitation affected the decolorization. Further studies were carried out to adjust the level of determinant factors. Results showed that color removal (62%) was optimum at 150 rpm and 3 g/l glucose. HPLC and FTIR analysis of the treated TOPW suggested that the decolorization occurs through biosorption and biodegradation. Tannase and lignin peroxidase were detected in the cultures. However, only tannase is responsible for the color removal of the TOPW because lignin peroxidase is produced at a low level. Data are reported as mean ± standard deviation of results carried out in triplicate Characteristics

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Section: Study Of Enzymes Responsible For Topw Decolorizationmentioning

confidence: 99%

“…The aerobic treatment of wastewaters was shown to be an encouraging way to reduce their toxicity and dark color [4][5][6]. The removal of color has been carried out by many fungal strains such as Aspergillus sp., which can decolorize [7][8][9][10] and / or biosorb recalcitrant compounds [11,12].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Biological Pretreatment of Green Table Olive Processing Wastewaters Using Aspergillus niger

Ayed¹,

Chammam²

2014

J Bioremed Biodeg

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“…3 The use of enzymes in waste treatment includes the decolorization of olive mill waste waters, 4 decolorization of an artificial textile effluent, 5 polymerization of pentachlorophenol, 6,7 degradation of azo, triphenyl and heterocyclic dyes, 8,9 and upgrading corn straw for paper pulping. 10 Mushrooms utilize a variety of lignocellulosic residues and convert them into human food.…”

Section: Introductionmentioning

confidence: 99%

Optimization of extraction of bulk enzymes from spent mushroom compost

Singh

Abdullah

Sabaratnam

2003

J of Chemical Tech & Biotech

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The profiling of ligninase, hemicellulase and cellulase of Pleurotus sajor-caju after inoculation of spawn in bags containing sawdust was done at monthly intervals for a period of 6 months. Xylanase (EC 3.2.1.8) was produced throughout the 6 months studied with the productivity range from 5.60 to 7.51 U g −1 . Cellulase (EC 3.2.1.4) and β-glucosidase (EC 3.2.1.21) productivities were highest at 4 months, producing 3.31 U g −1 and 121.13 U g −1 respectively. Laccase (EC 1.10.3.2) productivity was highest at 2 months with a value of 7.59 U g −1 . Lignin peroxidase (EC 1.11.1.14) productivity was highest at 5 months with a value of 206.20 U g −1 . Total soluble proteins were highest at 4 months with a value of 0.139 mg cm −3 . The profiling of lignin peroxidase in 5-month-old spent mushroom compost was monitored over a period of 10 months. It was observed that lignin peroxidase was produced throughout the period but productivity was variable. The average lignin peroxidase productivity ranged from 30 to 110 U g −1 . The activities of the enzymes extracted in tap water at pH 8.4 were comparable to that extracted in 50 mmol sodium citrate buffer at pH 4.8 and distilled water at pH 5.2 at 4• C using an incubator shaker at 200 rpm for 18 h. The optimum extraction time was 1 h using an incubator shaker at 4• C. When an incubator shaker was used, there was no significant difference in the recovery of xylanase, cellulase and laccase at different pH values at 4• C and 28 • C. No significant difference was observed in the recovery of β-glucosidase using an incubator shaker at different pH values at 4• C although the enzyme recovery was slightly higher at pH 8.12, with a value of 29.27 U g −1 . The optimum extraction of β-glucosidase was at pH 4 at room temperature using an incubator shaker. For the lignin peroxidase enzyme, the optimum pH for extraction was 6 at 4• C and pH 7 at room temperature using an incubator shaker at 200 rpm for 1 h. Homogenization for 8 min at 8000 rpm using tap water at pH 4 had an advantage over the use of the incubator shaker for the extraction as high titers of enzymes were recovered.

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“…Chrysosporium is one of the organisms whose ligninolytic enzyme system has been studied extensively. Because of its high ligninolytic activity, rapid growth, and ability to produce asexual spores, several biotechnological applications were tested using this particular species (Sayadi and Ellouz, 1992;1993).…”

Section: Introductionmentioning

confidence: 99%

“…A recent work has shown that the P. Chrysosporium species is able to degradate a wide variety of phenolic compounds (Sayadi and Ellouz, 1992;1993). Besides P. Chrysosporium produces two types of extra-cellular peroxidases involved in lignin biodegradation:…”

Section: Introductionmentioning

confidence: 99%

The biodegradation of Olive Oil Mill Wastewaters by Sawdust and by a <i>Phanerochaetae chrysosporium</i>

Mebirouk¹,

Sbai²,

López³

et al. 2007

Grasas y Aceites

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RESUMENLa biodegradación del alpechín por sawdust y por Phanerochaetae chrysosporium.Este trabajo describe la decoloración y la disminución de la demanda química de oxígeno del alpechín (OMW) por Phanerochaetae chrysosporium, crecido en cultivos estáti-cos, agitados e inmovilizados. Cuando P. chrysosporium fue cultivado en agitación, no se observa ninguna decoloración de OMW crudo, la decoloración ocurre solamente después de eliminar los polifenoles mediante adsorción en el serrín (Disminución del 39% del contenido en polifenoles). La utilización de la lignina peroxidasa generada en el medio da lugar a la mayor decoloración de alpechín y a las eficiencias de eliminación de DQO más altas. Las pruebas de la decoloración realizadas en las muestras de OMW que fueron pretratadas por la adsorción de madera del serrín, y usaron cultivos inmovilizadas demostraron resultados mejores. Por tanto, la eficiencia de eliminación de DQO alcanzó un 70%. La reducción de los polifenoles alcanzó los niveles más altos siempre, i.e. 62%. Se observó una decoloración significativa del efluente. PALABRAS-CLAVE: Alpechín -Biodegradación --Decoloración -Polifenoles -Serrín. SUMMARY The biodegradation of Olive Oil Mill Wastewaters by Sawdust and by a Phanerochaetae chrysosporium.This paper discusses decolorization and chemical oxygen demand (COD) abatement in olive mill wastewaters (OMW) by Phanerochaetae chrysosporium grown in static, stirred and immobilized cultures. When P. Chrysosporium is used in cultures, no decolorization of crude OMW is observed. Decolorization occurs only after the removal of polyphenols by adsorption in sawdust, which allows a 39% polyphenol removal. The use of a High lignin peroxides (Lip) producing medium, yields the highest OMW decolorization and COD removal efficiencies. The use of P. Chrysosporium immobilized on polyurethane foam leads to significant abatements of OMW polluting characteristics. And COD abatement reached 70%. The reduction of polyphenols reached its highest level at 62%. A significant effluent decolorization is apparent.

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Decolourization of olive mill waste-waters by the white-rot fungus Phanerochaete chrysosporium: involvement of the lignin-degrading system

Cited by 102 publications

References 25 publications

Optimization of Biological Pretreatment of Green Table Olive Processing Wastewaters Using Aspergillus niger

Optimization of Biological Pretreatment of Green Table Olive Processing Wastewaters Using Aspergillus niger

Optimization of extraction of bulk enzymes from spent mushroom compost

The biodegradation of Olive Oil Mill Wastewaters by Sawdust and by a <i>Phanerochaetae chrysosporium</i>

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