Decolourization and biodegradation of N,N′-dimethyl-p-phenylenediamine by Klebsiella pneumoniae RS-13 and Acetobacter liquefaciens S-1

Wong, Po Keung; Yuen, P Y

doi:10.1046/j.1365-2672.1998.00479.x

Cited by 60 publications

(28 citation statements)

References 23 publications

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“…Forty microlitres of the samples was analysed with a Hewlett Packard HPLC 1050 equipped with a model variable-wavelength detector (detection wavelength was 250 nm) and a reverse-phase Inertsil 5m ODS-2 column (4?66250 mm, MetaChem Technologies). The mobile phase was composed of 25 mM phosphate buffer (pH 3?0) and acetonitrile (4 : 6, v/v) with a flow rate of 0?5 ml min 21 (Moutaouakkil et al, 2003;Nakanishi et al, 2001;Wong & Yuen, 1998).…”

Section: Methodsmentioning

confidence: 99%

Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoprotein

2005

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Azo dyes are a predominant class of colourants used in tattooing, cosmetics, foods and consumer products. A gene encoding NADPH-flavin azoreductase (Azo1) from the skin bacterium Staphylococcus aureus ATCC 25923 was identified and overexpressed in Escherichia coli. RT-PCR results demonstrated that the azo1 gene was constitutively expressed at the mRNA level in S. aureus. Azo1 was found to be a tetramer with a native molecular mass of 85 kDa containing four non-covalently bound FMN. Azo1 requires NADPH, but not NADH, as an electron donor for its activity. The enzyme was resolved to dimeric apoprotein by removing the flavin prosthetic groups using hydrophobic-interaction chromatography. The dimeric apoprotein was reconstituted on-column and in free stage with FMN, resulting in the formation of a fully functional native-like tetrameric enzyme. The enzyme cleaved the model azo dye 2-[4-(dimethylamino)phenylazo]benzoic acid (Methyl Red) into N,N-dimethyl-p-phenylenediamine and 2-aminobenzoic acid. The apparent K m values for NADPH and Methyl Red substrates were 0?074 and 0?057 mM, respectively. The apparent V max was 0?4 mM min "1 (mg protein) "1 . Azo1was also able to metabolize Orange II, Amaranth, Ponceau BS and Ponceau S azo dyes. Azo1 represents the first azoreductase to be identified and characterized from human skin microflora.

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Section: Methodsmentioning

confidence: 99%

Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoprotein

2005

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“…Azo bonds present in these compounds are resistant to breakdown, with the potential for the persistence and accumulation in the environment [8]. However, they can be degraded by bacteria under aerobic and anaerobic conditions [9]. Several physico-chemical techniques have been proposed for treatment of colored textile effluents.…”

Section: Introductionmentioning

confidence: 99%

Microbial degradation of Textile Dye (Remazol Black B) by Bacillus spp. ETL-2012

Shah¹

2012

J Bioremed Biodeg

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“…The effluent from a dye-processing plant was shown to be responsible for the mutagenic activity detected in a Brazilian river [4]. Methyl red is mutagenic in nature, and most microbial degradation studies reveal the formation of N, N-dimethyl-phenylenediamine, a toxic and mutagenic aromatic amine [5] that remains untreated in the culture [6]. Acid violet 7 has a significant ability to induce chromosome aberrations, lipid peroxidation and inhibition of acetylcholinesterase.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification and Screening of Dye Decolorizing Bacteria

Shah¹,

Patel²,

Nair³

et al. 2013

AJMR

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The present study deals with the isolation, identification and screening of bacterial species capable to decolorize variety of dyes. Decolorization of dyes and growth of the bacterial species are investigated. The strain ETL-1942 decolorized all the selected dyes except Remazol Brilliant Blue R, Reactive Blue H5G, Remazol Turquoise Blue G and Fast Green. In our study, we identified three groups of dyes on the basis of the pattern of dye decolorization by strain ETL-1942. The results indicate that decolorization rate is faster for the dyes of the third group than the second group. The strain can grow with all the six dyes tested, but decolorization rates are different for each dye. These results demonstrate that the strain ETL-1942 have ability to decolorize wide range of dyes and therefore further investigation for physico-chemical parameter were carried out.

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Decolourization and biodegradation of N,N′-dimethyl-p-phenylenediamine by Klebsiella pneumoniae RS-13 and Acetobacter liquefaciens S-1

Cited by 60 publications

References 23 publications

Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoprotein

Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoprotein

Microbial degradation of Textile Dye (Remazol Black B) by Bacillus spp. ETL-2012

Isolation, Identification and Screening of Dye Decolorizing Bacteria

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