Decolourisation of a polymeric dye by selected fungal strains in liquid cultures

Rigas, Fotis; Dritsa, V.

doi:10.1016/j.enzmictec.2005.10.006

Cited by 35 publications

(28 citation statements)

References 24 publications

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“…Similar results [27] shown the difficulty for the I. lacteus fungus to eliminate mono or diazo dyes such as Orange G compared to anthraquinone dyes such as RB4. For a period of 18 days exposure, Rigas and Dritsa obtained rates decolorization of poly R-478 (100 mg/L) of 93.4% by G. australe, 66.25% by P. ostreatus sp.3 and 98.49% by P. sanguineus respectively [23]. Some of these values were similar to those obtained during this study despite a higher concentration and a shorter time (15 days).…”

Section: Decolorization and Enzyme Activities On Liquid Mediasupporting

confidence: 75%

“…These observations were completely different from those in Figure 7c (Orange G control) with two peaks at 3.1 min and 3.9 min and absorbance beyond 500 nm. Similar results were presented during a HPLC follow-up of dye biodegradation [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33]. The FTIR spectrum as well as the chromatogram of control (fungi alone) (not presented) were different from those of the degradation products (Figure 6b, 6d, 7b and 7d).…”

Section: Analysis Of Degradation Products By Ftir and Hplcsupporting

confidence: 63%

“…The results showed a significant decrease of decolorization with increasing dye concentration, as well as very important differences between the two dyes at the same concentrations ( Figure 1). In literature, the concentrations usually used are less than 0.2 g/L [4,9,[22][23][24]. In this study, a concentration of 0.3 g/L of RB4 was optimal with a decolorization rate of 98%.…”

Section: Effect Of Concentration and Temperature On Dyes Decolorizationmentioning

confidence: 78%

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Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Attéké

Mounguengui²,

Jean-Bosco

et al. 2013

J Bioremed Biodeg

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The fungus Pycnoporus sanguineus MUCL 51321 white rot isolated in Gabon showed a high ability to decolorize and degrade two synthetic dyes. On solid and liquid media, the fungus had different enzyme activities (laccase and manganese (Mn) peroxidase) in the presence of different substrates. At concentrations of 0.05 g/L (Orange G) and 0.3 g/L (reactive blue 4), the respective rates of decolorization of 81% and 97% were observed after 15 days of incubation in liquid media. In the same time interval, changes on the spectra (UV-vis and FTIR) and chromatograms (HPLC) showed that these decolorizations were due to the degradation of dyes by fungus with the production of new compounds. The study revealed the possibility of the use of this fungal strain in the microbial degradation process of synthetic dyes. Aldrich (Sigma-Aldrich, St. Louis, MO, USA). All reagents used were of the analytical grade. Journal of Bior emediation & Biodegradation Culture ConditionsThe composition of the solid growth medium used was as follows: 0.2% glucose, 0.2% malt extract, 0.01% MgSO 4 , 0.01% MnCl 2 , 0.03% NH 4 NO 3 , 0.026% KH 2 PO 4 , 0.026% Na 2 HPO 4 , 0.01% CaCl 2 .2H 2 O, 0.0001% FeC1 3 .6H 2 O, 0.0001% ZnCl 2 , 1.6% and 1.6% agar in 1 L of distilled water. The pH was adjusted to 5.5 with hydrochloric acid (0. 5 N) before autoclaving (at 121°C for 15 min). The culture medium was transferred into 4 Erlenmeyer flasks (200 mL each) and each reagent (enzymatic revelation) or dye, depending on its final concentration was added. The mixture was shaken, then about 15 mL were distributed in a Petri dish (diameter 8.5 mm); each test was replicated three times. 50 mL of liquid culture media in flasks of 100 mL were constituted in almost the same way as the solid culture media described above, except for the absence of agar. Each test was conducted three times. Decolorization experimentsDyes decolorization on solid medium: Solid culture media contained different concentrations of each dye (0.05 g/L to 1.5 g/L). Various controls were also prepared: some Petri dishes with fungus alone and others with dye alone to assess possible abiotic decolorization. One square portion of agar (1 cm of diameter) containing the fungus was placed at the centre of each Petri dish. The fungal growth diameters and the halos of decolorization were measured every day during one week at constants temperature (30°C) and humidity (75%). After these measures, different ratios and rates (decolorization or inhibition) were determined as described by . The optimum temperature of decolorization was evaluated using different growth temperatures (15, 22, 30, 35, 40 and 45°C). Moreover, the optimal concentrations for decolorization (0.05 g/L for the Orange G and 0.3 g/L for the RB4) were obtained after one week. All tests were conducted three times (averages are presented with the corresponding standard deviations).Dyes decolorization on liquid culture medium: For each Erlenmeyer flask (100 mL) containing liquid culture medium (50 mL) and dye (0.05 g/L Orange G or 0.3...

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Section: Decolorization and Enzyme Activities On Liquid Mediasupporting

confidence: 75%

Section: Analysis Of Degradation Products By Ftir and Hplcsupporting

confidence: 63%

Section: Effect Of Concentration and Temperature On Dyes Decolorizationmentioning

confidence: 78%

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Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Attéké

Mounguengui²,

Jean-Bosco

et al. 2013

J Bioremed Biodeg

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“…A reduction of the initial pH of the culture medium from 5.0 to 3.6 was also observed during the growth phase (first 12 days) of Trametes pubescens (Galhaup et al, 2002). Rigas and Dritsa (2006) observed redution of the pH from 6.7 to 4-5 during the growth of Ganoderma australe, Polyporus brumalis and P. ciliatus. The use of dyes as model compounds in pollutant biodegradation studies offers a series of advantages compared to conventional substrates: dyes are stable, soluble, possess high molar extinction coefficients and low toxicity, and can be applied in simple, rapid and quantitative spectrophotometric assays.…”

Section: Resultsmentioning

confidence: 77%

Influence of pH on the growth, laccase activity and RBBR decolorization by tropical basidiomycetes

Neto

Matheus

Machado

2009

Braz. arch. biol. technol.

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The basidiomycete fungi Lentinus crinitus and Psilocybe castanella are being evaluated in a bioremediation process of soils contaminated with organochlorine industrial residues in the Baixada Santista, São Paulo. The aim of the present study was to determine the influence of pH on the fungal growth, in vitro decolorization of anthraquinonic dye Remazol Brilliant Blue R (RBBR) and laccase activity. The pH of the culture medium influenced the growth of L. crinitus and P. castanella, which presented less growth at pH 5.9 and pH 2.7, respectively. The fungi were able to modify the pH of the culture medium, adjusting it to the optimum pH for growth which was close to 4.5. Decolorization of the RBBR was maximal at a pH of 2.5 to 3.5. Higher laccase activity was observed at pH 3.5 and pH 4.5 for L. crinitus and P. castanella, respectively. pH was found to be an important parameter for both the growth of these fungi and the enzymatic system involved in RBBR decolorization.

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“…However, in the available literature lacks the information about decolorization of alizarin blue by microorganisms, whereas data are available that indicate the decolorization of other anthraquinone dyes by white-and brown-rot fungi: Remazol Brillant Blue R (RBBR), Basic Blue 22, Poly R-478 (a polyanthraquinone dye) (Freitag and Morrell 1992;Jarosz-Wilkołazka et al 2002;Eichlerová et al 2005Eichlerová et al , 2007Lozovaya et al 2006;Rigas and Dritsa 2006;Raju et al 2007;Anastasi et al 2009;Yemendzhiev et al 2009;Korniłłowicz-Kowalska and Rybczyńska 2012). Based on our study, we concluded that certain of the selected strains of microfungi, e.g., H. haematococca and C. rosea f. catenulata, already after 4 days, while T. harzianum after 2 days, caused 40, 50, and 65 %, respectively, decolorization of 0.03 % solutions of the monoanthraquinone dye used.…”

Section: Decolorization Activity In Liquid Culturesmentioning

confidence: 99%

Screening of microscopic fungi and their enzyme activities for decolorization and biotransformation of some aromatic compounds

Korniłłowicz-Kowalska

Rybczyńska

2014

Int. J. Environ. Sci. Technol.

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A total of 610 strains from 16 species of micromycetes with potential ligninolytic capabilities were isolated from soil, compost, and rotten wood, including 400 strains of soil origin. In the test of decolorization of agarized 0.06 % alizarin blue, 52 strains with high decolorization activity were selected. In liquid cultures with 0.2 % post-industrial lignin and 0.03 % alizarin blue, all of the selected strains showed three different extracellular peroxidase activities such as HRP-like, LiP, and MnP. Laccase was detected only in the cultures with alizarin blue, but those were trace amounts. In the decolorization of alizarin blue, the highest levels of activity were attained by HRPlike peroxidase. In the transformations of post-industrial lignin that included decolorization, colorization (darkening of substrate), and re-colorization, the highest levels of activity were attained by lignin peroxidase, especially at the start of the culture. The transformations of both dye substrates in the cultures of all 52 strains were accompanied by the release of glucose oxidase, but high activities of that enzyme were observed only in the presence of alizarin blue. Whereas in the presence of lignin the fungi under study synthesized catalase, the activity of which attained a maximum toward the end of the culture was negatively correlated with the activity of the remaining oxidoreductases under study. Among the 52 selected strains, three strains such as Haematonectria haematacocca BwIII43, K37, and Trichoderma harzianum BsIII33 were characterized by a high activity of decolorization.

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Decolourisation of a polymeric dye by selected fungal strains in liquid cultures

Cited by 35 publications

References 24 publications

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Influence of pH on the growth, laccase activity and RBBR decolorization by tropical basidiomycetes

Screening of microscopic fungi and their enzyme activities for decolorization and biotransformation of some aromatic compounds

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