Decolourisation of a pigment plant effluent by Pycnoporus cinnabarinus in a packed-bed bioreactor

Schliephake, Kirsten; Lonergan, Greg T.; Jones, Cameron L.; Mainwaring, David E.

doi:10.1007/bf00131213

Cited by 54 publications

(15 citation statements)

References 12 publications

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“…The medium was then autoclaved at 103.4 kilopascals for 15 min with or without other additives. The newspaper was not treated to remove the ink as one of the reasons for producing the laccase was to decolourise the industrial dyes, 5,6 especially those used on newspaper.…”

Section: Microbiological Proceduresmentioning

confidence: 99%

“…Since the objective of this work was to increase laccase production on a large scale for use in newspaper degradation and dye decolouration, 5,6 the unshaken ask experiments were scaled up to a bioreactor stage using a complex medium. 13 Under these conditions laccase was produced at higher levels and for longer times.…”

Section: Laccase Production In a Packed Bed Reactormentioning

confidence: 99%

“…5 Thirty grams of shredded newspaper (in approx 300±500 mm Â 5±10 mm strips) was used as the packing for the reactor. 3 .…”

Section: Fermentation Experimentsmentioning

confidence: 99%

“…3±5 This fungus has been used in degradative studies on agricultural and industrial wastes and decolourisation of dyes. 5 In the latter case the dye was actually degraded by the puri®ed laccase. 6 Pyc cinnabarinus and its laccase are the subject of a continuing investigation in this laboratory, particularly in relation to the degradation of wastes that include recycled newspaper.…”

Section: Introductionmentioning

confidence: 99%

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Dimethylsulfoxide elevates extracellular laccase (phenol oxidase) activity ofPycnoporus cinnabarinusgrown on a low nutritional newspaper medium

Jones

Baker

Lonergan

2001

J of Chemical Tech & Biotech

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The white-rot fungus Pycnoporus cinnabarinus, grown on a low nutrient newspaper medium, secreted low amounts of extracellular laccase. The laccase activity was increased when 3% methanol was added to the medium and further increased when 3% dimethylsulfoxide was added. Dimethylsulfoxide increased the enzyme activity over controls when the fungus was grown on a malt extract broth, or media containing either recycled Kraft paper or cellobiose. Treatment with the ionophore A23187 suggested that dimethylsulfoxide acted as a paramorphogen to increase the branch points of the mycelial mat. Hence, the number of ion channels for secretion of the enzyme was increased. When the fungus was grown in a 2 dm 3 bioreactor, sparged with air, the measured extracellular protein concentration rose and remained relatively steady for 15 days. The enzyme activity and speci®c activity reached a maximum between days 6 and 9 and then fell from day 10 of fermentation. The presence of dimethylsulfoxide in the bioreactor caused a four-fold increase in enzyme activity and prolonged its secretion in the bioreactor for at least 25 days. The protein concentration and speci®c activity also remained at high levels over this period.

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Section: Microbiological Proceduresmentioning

confidence: 99%

Section: Laccase Production In a Packed Bed Reactormentioning

confidence: 99%

“…5 Thirty grams of shredded newspaper (in approx 300±500 mm Â 5±10 mm strips) was used as the packing for the reactor. 3 .…”

Section: Fermentation Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dimethylsulfoxide elevates extracellular laccase (phenol oxidase) activity ofPycnoporus cinnabarinusgrown on a low nutritional newspaper medium

Jones

Baker

Lonergan

2001

J of Chemical Tech & Biotech

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show abstract

“…No colour removal was observed in an uninoculated control or when yeast-extract was omitted. The ability of PDW to decolourise textile effluent would appear to be comparable with that seen when a strain of Pycnoporus cinnabarinus was used to treat pigment plant effluent (Schliephake et al, 1993) and is much more rapid than the rate of microbial decolourisation of several industrial effluents observed by Zhou and Zimmermann (1993). Future work will concentrate on using PDW in the development of a laboratory-based textile effluent treatment system.…”

Section: Decolourisation Of Textile Effluent By Pdwmentioning

confidence: 99%

Decolourisation of effluent from the textile industry by a microbial consortium

Nigam¹,

McMullan²,

Banat³

et al. 1996

Biotechnol Lett

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Study of dye decolorization in an immobilized laccase enzyme‐reactor using online spectroscopy

Kandelbauer

Maute

Kessler

et al. 2004

Biotech & Bioengineering

128

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Decolorization of textile dyes by a laccase from Trametes modesta immobilized on g-aluminum oxide pellets was studied. An enzyme reactor was equipped with various UV/Vis spectroscopic sensors allowing the continuous online monitoring of the decolorization reactions. Decolorization of the dye solutions was followed via an immersion transmission probe. Adsorption processes were observed using diffuse reflectance measurements of the solid carrier material. Generally, immobilization of the laccase does not seem to sterically affect dye decolorization. A range of commercial textile dyes was screened for decolorization and it was found that the application of this enzymatic remediation system is not limited to a certain structural group of dyes. Anthrachinonic dyes (Lanaset Blue 2R, Terasil Pink 2GLA), some azo dyes, Indigo Carmine, and the triphenylmethane dye Crystal Violet were efficiently decolorized. However, the laccase displayed pronounced substrate specificities when a range of structurally related model azodyes was subjected to the biotransformation. Azodyes containing hydroxy groups in ortho or para position relative to the azo bond were preferentially oxidized. The reactor performance was studied more closely using Indigo Carmine.

show abstract

Decolourisation of a pigment plant effluent by Pycnoporus cinnabarinus in a packed-bed bioreactor

Cited by 54 publications

References 12 publications

Dimethylsulfoxide elevates extracellular laccase (phenol oxidase) activity ofPycnoporus cinnabarinusgrown on a low nutritional newspaper medium

Dimethylsulfoxide elevates extracellular laccase (phenol oxidase) activity ofPycnoporus cinnabarinusgrown on a low nutritional newspaper medium

Decolourisation of effluent from the textile industry by a microbial consortium

Study of dye decolorization in an immobilized laccase enzyme‐reactor using online spectroscopy

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