Decolourisation Capabilities of Ligninolytic Enzymes Produced byMarasmius cladophyllusUMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes

Abstract

Marasmius cladophyllus was examined for its ability to degradatively decolourise the recalcitrant dye Remazol Brilliant Blue R (RBBR) and screened for the production of ligninolytic enzymes using specific substrates. Monitoring dye decolourisation by the decrease in absorbance ratio of A592/A500 shows that the decolourisation of RBBR dye was associated with the dye degradation. Marasmius cladophyllus produces laccase and lignin peroxidase in glucose minimal liquid medium containing RBBR. Both enzyme activities were increased, with laccase activity recorded 70 times higher reaching up to 390 U L−1 on day 12. Further in vitro RBBR dye decolourisation using the culture medium shows that laccase activity was correlated with the dye decolourisation. Fresh RBBR dye continuously supplemented into the decolourised culture medium was further decolourised much faster in the subsequent round of the RBBR dye decolourisation. In vitro dye decolourisation using the crude laccase not only decolourised 76% of RBBR dye in just 19 hours but also decolourised 54% of Orange G and 33% of Congo red at the same period of time without the use of any exogenous mediator. This rapid dye decolourisation ability of the enzymes produced by M. cladophyllus thus suggested its possible application in the bioremediation of dye containing wastewater.

Decolorization kinetics and mass transfer mechanisms of Remazol Brilliant Blue R dye mediated by different fungi

“…strain produces a color change from dark blue before to light color of liquid culture medium after 7 d of incubation. The decolorization ability of ligninolytic enzymes produced by Marasmius cladophyllus can be proposed for the bioremediation of RBBR dye contained in the textile industry wastewater [ 44 ]. The results ( Figs.…”

Ligninolytic Enzyme Production and Decolorization Capacity of Synthetic Dyes by Saprotrophic White Rot, Brown Rot, and Litter Decomposing Basidiomycetes

“…Some authors reported that laccase plays the primary role in Orange G decolorization, while MnP production is more significant for RBBR decolorization [ 20 , 69 , 86 , 87 , 88 ]; nevertheless, others consider MnP to be essential for both azo and anthraquinone dye decolorization [ 31 , 89 , 90 , 91 ]. On the other hand, some authors declared the crucial role of laccase in the decolorization of RBBR [ 62 , 67 , 68 ] or in the decolorization of both anthraquione and azo dyes [ 28 , 49 ]. Although it is widely known that the ligninolytic enzymes laccase and MnP produced by saprotrophic fungi play a crucial role in the degradation of various xenobiotics, including dyes [ 92 ], the process of their degradation is more complicated and many other factors (different mediators, radicals, hydrogen peroxide, other oxidative enzymes, and so on) participate in it [ 7 , 17 , 18 , 93 , 94 ].…”

Notes Some Macro Fungi From Taman Eden 100, Kawasan Toba, Sumatera Utara, Indonesia: Description and Its Potency

“…Marasmius cladophyllus were demonstrated by Sing et al (2017). In their study, Marasmius cladophyllus produced a laccase enzyme that able to degraded those recalcitrant dyes.…”

“…Some species of fungi also form a mutualistic symbiosis with plant roots and form mycorrhiza structures (Pointing et al, 2005;Lehto & Zwiazek, 2011;Lenoir et al, 2016;Guerrero-galán et al, 2019). Besides, several species of fungi are also Doi: https://doi.org/10.22236/j.bes/515326 ISSN: 2614-1558 | 31 applied for biotechnology purposes, and the results of its applications are developed extensively in several industries areas such as food, agriculture, textile, medicine, and wastewater treatment (Sing et al, 2017;Anita et al, 2019;Hyde et al, 2019). As a eukaryotes organism, fungi can be found in both macroscopic and microscopic forms.…”