Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans

Kim, Yong‐Hak; Lee, Cheolju; Go, Hayoung; Konishi, Kyoko; Lee, Kangseok; Lau, Peter C. K.; Yu, Myeong-Hee

doi:10.1016/j.abb.2009.11.027

Cited by 20 publications

(6 citation statements)

References 35 publications

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“…Isolated mitochondria from skeletal muscle tissues and cybrid cells were dissolved in 4% SDS lysis buffer in Tris HCl at pH 7.6, and the solution was transferred into 1.5-ml e-tubes containing 2-mm glass beads. The mitochondria were disrupted by repeated strokes at 1000 rpm for 30 s using a Mini-BeadBeater TM Mill (Cole-Parmer Instrument, Vernon Hills, IL) and cooled on ice for 1 min 17 . This procedure was repeated until no pellets were visible (~10–15 times).…”

Section: Methodsmentioning

confidence: 99%

A mitochondrial proteome profile indicative of type 2 diabetes mellitus in skeletal muscles

et al. 2018

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The pathogenesis of type 2 diabetes mellitus (T2DM) is closely associated with mitochondrial functions in insulin-responsive tissues. The mitochondrial proteome, compared with the mitochondrial genome, which only contains 37 genes in humans, can provide more comprehensive information for thousands of mitochondrial proteins regarding T2DM-associated mitochondrial functions. However, T2DM-associated protein signatures in insulin-responsive tissues are still unclear. Here, we performed extensive proteome profiling of mitochondria from skeletal muscles in nine T2DM patients and nine nondiabetic controls. A comparison of the mitochondrial proteomes identified 335 differentially expressed proteins (DEPs) between T2DM and nondiabetic samples. Functional and network analyses of the DEPs showed that mitochondrial metabolic processes were downregulated and mitochondria-associated ER membrane (MAM) processes were upregulated. Of the DEPs, we selected two (NDUFS3 and COX2) for downregulated oxidative phosphorylation and three (CALR, SORT, and RAB1A) for upregulated calcium and protein transport as representative mitochondrial and MAM processes, respectively, and then confirmed their differential expression in independent mouse and human samples. Therefore, we propose that these five proteins be used as a potential protein profile that is indicative of the dysregulation of mitochondrial functions in T2DM, representing downregulated oxidative phosphorylation and upregulated MAM functions.

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Section: Methodsmentioning

confidence: 99%

A mitochondrial proteome profile indicative of type 2 diabetes mellitus in skeletal muscles

et al. 2018

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“…In addition, strains of Cunninghamella sp. were characterized as able to degrade a wide range of dyes (Ambrosio and Campos-Takaki, 2004;Kim et al, 2010), suggesting potential biodegradative capacities towards hydrocarbon compounds.…”

Section: Assessment Of the Biodegradation Potential Of Hydrocarbons Imentioning

confidence: 99%

Bioaugmentation and biostimulation as strategies for the bioremediation of a burned woodland soil contaminated by toxic hydrocarbons: A comparative study

Andreolli

Lampis

Brignoli³

et al. 2015

Journal of Environmental Management

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“…Primarily, a previous study reported that c-type heme-containing protein from Cunninghamella elegans was able to decolorize MG in the presence of mitochondria [32]. MG also led to the inhibition of mitochondrial and lysosomal activity in rat FaO and L6 cell lines [33].…”

Section: Mitochondriamentioning

confidence: 99%

Comparative Proteomic Analysis Provides Insight into the Key Proteins as Potential Targets Involved in Malachite Green Inhibiting Reproduction of Ichthyophthirius Multifiliis

Qu¹,

Song²,

Tan³

et al. 2020

Preprint

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Background: Target identification is important for drug discovery. Unfortunately, no drug targets have been found in I. multifiliis until now and further limited development of the novel drug for Ichthyophthiriasis. Methods: In this study, an iTRAQ-based quantitative proteomic analysis was used to find the target of malachite green (MG), exhibiting greater efficacy than the existing drugs, against I. multifiliis trophonts in situ. We also verified the proteomic results by RT-qPCR, TEM, cell apoptosis assay.Results: Our results showed that major variations in protein abundance were found among many of the ribosome proteins, indicating ribosome might be a candidate target. Furthermore, GO and KEGG pathway analyses of differentially expressed proteins (DEPs) revealed that ribosome and PI3K-Akt signaling pathway were remarkably enriched. Taken together, the above DEPs were also verified by RT-qPCR and morphological observations. Conclusions: This study provides insights into the key proteins enriched in PI3K-Akt signal pathway and ribosome pathway as potential targets of MG inhibiting reproduction of I. multifiliis, which could be serve as targets for other less toxic drugs and be tested as potential treatments for I. multifiliis.

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Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans

Cited by 20 publications

References 35 publications

A mitochondrial proteome profile indicative of type 2 diabetes mellitus in skeletal muscles

A mitochondrial proteome profile indicative of type 2 diabetes mellitus in skeletal muscles

Bioaugmentation and biostimulation as strategies for the bioremediation of a burned woodland soil contaminated by toxic hydrocarbons: A comparative study

Comparative Proteomic Analysis Provides Insight into the Key Proteins as Potential Targets Involved in Malachite Green Inhibiting Reproduction of Ichthyophthirius Multifiliis

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Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans

Cited by 20 publications

References 35 publications

A mitochondrial proteome profile indicative of type 2 diabetes mellitus in skeletal muscles

A mitochondrial proteome profile indicative of type 2 diabetes mellitus in skeletal muscles

Bioaugmentation and biostimulation as strategies for the bioremediation of a burned woodland soil contaminated by toxic hydrocarbons: A comparative study

Comparative Proteomic Analysis Provides Insight into the Key Proteins as Potential Targets Involved in Malachite Green Inhibiting Reproduction of&nbsp;Ichthyophthirius Multifiliis

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Comparative Proteomic Analysis Provides Insight into the Key Proteins as Potential Targets Involved in Malachite Green Inhibiting Reproduction of Ichthyophthirius Multifiliis