Decolorization of diazo dye Direct Red 81 by a novel bacterial consortium

Abstract

Samples collected from various effluent-contaminated soils in the vicinities of dyestuff manufacturing units of Ahmedabad, India, were studied for screening and isolation of organisms capable of decolorizing textile dyes. A novel bacterial consortium was selected on the basis of rapid decolorization of Direct Red 81 (DR 81), which was used as model dye. The bacterial consortium exhibited 90% decolorization ability within 35 h. Maximum rate of decolorization was observed when starch (0.6 g l )1 ) and casein (0.9 g l )1 ) were supplemented in the medium. Decolorization of DR 81 was monitored by high performance thin layer chromatography, which indicated that dye decolorization was due to its degradation into unidentified intermediates. The optimum dye-decolorizing activity of the culture was observed at pH 7.0 and incubation temperature of 37°C. Maximum dye-decolorizing efficiency was observed at 200 mg l )1 concentration of DR 81. The bacterial consortium had an ability to decolorize nine other structurally different azo dyes.

Decolorization of Direct Blue GLL with enhanced lignin peroxidase enzyme production in Comamonas sp UVS

“…This might be due to either substrate inhibition, which may occur at high dye concentrations, 32 or increased toxicity of dye to cells or inadequate biomass for decolorization. 33,34 Decolorization of repeated addition of dye aliquots The repeated use of the microorganism is important from the commercial point of view. 35 In this study, the same culture of Comamonas sp UVS was used repeatedly for the decolorization of DBGLL.…”

Evaluation of Microbial Systems for Biotreatment of Textile Waste Effluents in Nigeria: Biodecolourization and Biodegradation of textile Dye

“…A great number of these fungi (especially white-rot fungi) have been shown to excrete extracellular enzymes like lignin peroxidase, manganese peroxidase and laccase (Hatakka, 1994) which catalyze the formation of activated oxygen so that the process of attack on the stable structure of dyes can be initiated. The role of some bacterial and algal species for the decolourization and degradation of textile dyes has also been reported (Jumarkar et al, 2006;Olukanni et al, 2006;Pourbabaee et al, 2006;Togo et al, 2008;Cheriaa et al, 2009). Dyes are removed by biosorption (Fu and Viraraghavan, 2000) and enzymatic mineralization (degradation) by lignin peroxidase, manganese peroxidase, manganese independent peroxidase and laccases (Raaghukumar et al, 1996;Duran et al, 2002;Wesenberg et al, 2003;Svobodova et al, 2006).…”

Solid State Fermentation of Wheat Straw for Production of MNP by P. Chrysoporium MTCC 787.

“…Azo dyes are the most common synthetic dyes released into the environment during their usage (Saratale et al, 2009). For sulfonated azo dyes, both aromatic sulfonic and azo groups confer to their xenobiotic nature as these are rare among natural products (Junnarkar et al, 2006).…”