Decolorization of Color Index Acid Orange 20 buffer solution using horseradish peroxidase immobilized on modified PAN-beads

Abstract

In the present work, horseradish peroxidase (HRP) is utilized to be immobilized onto polyacrylonitrile based beads (PAN-beads) for decolorization of Color Index (C. I.) Acid Orange 20 (AO20) in aqueous solution.Sodium hydroxide and hydrochloric acid were used to treat PAN-beads to obtain carboxyl groups, followed by being modified with ethanediamine to get more amino groups, and then were activated with glutaraldehyde. The enzyme was immobilized onto the activated beads by covalent crosslinking.Optimum factors for decolorization of AO20 were investigated both on free horseradish peroxidase (F-HRP) and immobilized horseradish peroxidase (I-HRP), including pH values, H 2 O 2 amount, enzyme amount, temperature and so on. Under optimum conditions, percent decolorization was about 90% or 98% respectively for F-HRP or I-HRP. A kinetic study showed that F-HRP possessed more affinity compared to I-HRP, but reusability experiments clarified that I-HRP was cost-efficient as about 90% decolorization was obtained after three cycles. The human normal hepatocytes cell line (L02 cell line) was utilized to assess cytotoxicity of metabolites, and the result was acceptable.

Functionalized Titania Nanosheet Dispersions of Peroxidase Activity

“…Enzymes, on the other hand, are sensitive biocatalysts that can partially or totally lose their enzymatic activity as a consequence of any changes occurring in their surrounding environment (e.g., pH, temperature, pressure, or ionic strength). , This major drawback of their environmental response can be overcome by immobilization on solid supports. ,− However, depending on the enzymes and the supports used, contradictory results were reported. ,− In some cases, immobilized enzymes performed better in harsher conditions compared to their native form in solution, whereas in some situations, immobilization resulted in denaturation over time.…”

Hemin-bound cysteinyl bolaamphiphile self-assembly as a horseradish peroxidase-mimetic catalyst

“…Interestingly, the catalytic activity of the HRP-mimetic catalyst continuously increased with increasing temperature up to 45 C (Fig. 3b), in contrast with the natural HRP activity, which decreases at temperatures above 38 C. [26][27][28] The temperature dependency is a non-biological character of the HRP-mimetic catalyst, although the activity did not increase further at temperatures above 45 C. This non-biological behaviour is presumably due to the thermal stability of the Cys-C7 assembly structure and solid hemin binding through the cysteinate-Fe bonding. On the other hand, the structure of HRP is destabilized above $40 C, resulting in reduced catalytic activity.…”

Teksti̇l Atik Suyunda HRP-Clea İle Hi̇drojen Peroksi̇di̇n Gi̇deri̇lmesi̇ İçi̇n Ölçek Büyütme Tasariminin Modellenmesi̇ Ve Si̇mülasyonu

“…Research (Sekuljica, Prlainovic, Jakovetic, Grbavcic, Ognjanovi, Knezevi-Jugovic ve Mijin, 2016;Opwis, Kiehl ve Gutmann, 2016;Yinca, Yan, Xueyong, Qiao ve Xiaoping, 2017) veya ÇBEA formları (Grateron, Barbosa, Ruedaa, Ortiz-L´opez ve Torres, 2007;Mehde, 2019;Kulkarni, Kadam, Jeon ve Govindwar, 2020) ile araştırmalar yapılmıştır. Etanol varlığında sığır serum albumini (BSA) olmadan ve BSA varken oluşturulan agregatlar çapraz bağlanmış ve üç azo boyanın renk giderimi sıcaklık ve pH için optimize edilmiştir.…”