Decoding Stability and Epistasis in Human Myoglobin by Deep Mutational Scanning and Codon-level Machine Learning
Christoph Küng,
Olena Protsenko,
Rosario Vanella
et al.
Abstract:Understanding the linkage between protein sequence and phenotypic expression level is crucial in biotechnology. Machine learning algorithms trained with deep mutational scanning (DMS) data have significant potential to improve this understanding and accelerate protein engineering campaigns. However, most machine learning (ML) approaches in this domain do not directly address effects of synonymous codons or positional epistasis on predicted expression levels. Here we used yeast surface display, deep mutational … Show more
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