Decoding Microbial Responses to Ammonia Shock Loads in Biogas Reactors through Metagenomics and Metatranscriptomics
Maria Gaspari,
Gabriele Ghiotto,
Victor Borin Centurion
et al.
Abstract:The presence of elevated ammonia levels is widely recognized as a significant contributor to process inhibition in biogas production, posing a common challenge for biogas plant operators. The present study employed a combination of biochemical, genome-centric metagenomic and metatranscriptomic data to investigate the response of the biogas microbiome to two shock loads induced by single pulses of elevated ammonia concentrations (i.e., 1.5 g NH 4 + /L R and 5 g NH 4 + /L R ). The analysis revealed a microbial c… Show more
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