Decoding ceRNA regulatory network and autophagy-related genes in benign prostatic hyperplasia

Zhou, Liang; Li, Youyou; Li, Jiaren; Yao, Hanyu; Huang, Jin; Li, Chen; Wang, Long

doi:10.1016/j.ijbiomac.2022.11.162

Cited by 10 publications

(6 citation statements)

References 56 publications

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“…We know that ceRNAs regulate many biological processes, including the cell cycle, tumor suppression, and tumor development 37–41 . For instance, lncRNAs are associated with various cancers like colorectal, gastric, oral, and so on by playing a role as ceRNA 42–46 through modulation of various tumorigenesis processes, such as cell proliferation, migration, and drug resistance 37 .…”

Section: Resultsmentioning

confidence: 99%

“…We know that ceRNAs regulate many biological processes, including the cell cycle, tumor suppression, and tumor development. [37][38][39][40][41] For instance, lncRNAs are associated with various cancers like colorectal, gastric, oral, and so on by playing a role as ceRNA [42][43][44][45][46] through modulation of various tumorigenesis processes, such as cell proliferation, migration, and drug resistance. 37 Therefore, these findings helped us to understand the function of dysregulated lncRNAs, possibly ceRNAs and their target genes, and how these ceR-NAs might be contributing to TMZ resistance in GBM.…”

Section: Hub Genes Modulated By Lncrnas As Cerna Involved In Tmz Resi...mentioning

confidence: 99%

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LncRNA‐associated competing endogenous RNA network analysis uncovered key lncRNAs involved in temozolomide resistance and tumor recurrence of glioblastoma

Nayak,

Mallick

2023

J of Molecular Recognition

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Temozolomide (TMZ) is a common alkylating chemotherapeutic agent used to treat brain tumors such as glioblastoma multiforme (GBM) and anaplastic astrocytoma. GBM patients develop resistance to this drug, which has an unclear and complicated molecular mechanism. The competing endogenous RNAs (ceRNAs) play critical roles in tumorigenesis, drug resistance, and tumor recurrence in cancers. This study aims to predict ceRNAs, their possible involvement, and underlying molecular mechanisms in TMZ resistance. Therefore, we analyzed coding and non‐coding RNA expression levels in TMZ‐resistant GBM samples compared to sensitive GBM samples and performed pathway analysis of mRNAs differentially expressed (DE) in TMZ‐resistant samples. We next applied a mathematical model on 950 DE long non‐coding RNAs (lncRNAs), 116 microRNAs (miRNAs), and 7977 mRNAs and obtained 10 lncRNA‐associated ceRNAs that may be regulating potential target genes involved in cancer‐related pathways by sponging 25 miRNAs in TMZ‐resistant GBM. Among these, two lncRNAs named ARFRP1 and RUSC2 regulate five target genes (IRS1, FOXG1, GNG2, RUNX2, and CACNA1E) involved in AMPK, AKT, mTOR, and TGF‐β signaling pathways that activate or inhibit autophagy causing TMZ resistance. The novel lncRNA‐associated ceRNA network predicted in GBM offers a fresh viewpoint on TMZ resistance, which might contribute to treating this malignancy.

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Section: Resultsmentioning

confidence: 99%

Section: Hub Genes Modulated By Lncrnas As Cerna Involved In Tmz Resi...mentioning

confidence: 99%

LncRNA‐associated competing endogenous RNA network analysis uncovered key lncRNAs involved in temozolomide resistance and tumor recurrence of glioblastoma

Nayak,

Mallick

2023

J of Molecular Recognition

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“…Immunofluorescence staining was performed as previously described [ 22 ]. For paraffin-embedded human PRAD or paracancer samples, 5-μm sections were deparaffinized with xylene and ethanol.…”

Section: Methodsmentioning

confidence: 99%

Epigenetic-related gene-based prognostic model construction and validation in prostate adenocarcinoma

Li,

et al. 2024

Heliyon

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“…Autophagy is a key process for the regular maintenance and disposal of intracellular organelles and proteins [34,35] and is involved in BPH development. [36,37] Moreover, FOXK1 is a transcriptional repressor of autophagy and its inhibition has been related to enhancing autophagy. [38,39] Hence, the effects of the miR-765/ FOXK1 axis on autophagy-related proteins LC3, Beclin-1 and ATG5 in TGF-stimulated BPH-1 cells were investigated (Figure 6G).…”

Section: Mir-765 Directly Targets Foxk1 To Inhibit Foxk1 Expressionmentioning

confidence: 99%

MIR663AHG as a competitive endogenous RNA regulating TGF‐β‐induced epithelial proliferation and epithelial–mesenchymal transition in benign prostate hyperplasia

Tong

et al. 2023

J Biochem & Molecular Tox

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Benign prostate hyperplasia (BPH) is the most commonly seen disease among aging males. Transforming growth factor(TGF)‐β‐mediated epithelial–mesenchymal transition (EMT) and epithelial overproliferation might be central events in BPH etiology and pathophysiology. In the present study, long noncoding RNA MIR663AHG, miR‐765, and FOXK1 formed a competing endogenous RNAs network, modulating TGF‐β‐mediated EMT and epithelial overproliferation in BPH‐1 cells. miR‐765 expression was downregulated in TGF‐β‐stimulated BPH‐1 cells; miR‐765 overexpression ameliorated TGF‐β‐mediated EMT and epithelial overproliferation in BPH‐1 cells. MIR663AHG directly targeted miR‐765 and negatively regulated miR‐765; MIR663AHG knockdown also attenuated TGF‐β‐induced EMT and epithelial overproliferation in BPH‐1 cells, whereas miR‐765 inhibition attenuated MIR663AHG knockdown effects on TGF‐β‐stimulated BPH‐1 cells. miR‐765 directly targeted FOXK1 and negatively regulated FOXK1. FOXK1 knockdown attenuated TGF‐β‐induced EMT and epithelial overproliferation and promoted autophagy in BPH‐1 cells, and partially attenuated miR‐765 inhibition effects on TGF‐β‐stimulated BPH‐1 cells. In conclusion, this study provides a MIR663AHG/miR‐765/FOXK1 axis modulating TGF‐β‐induced epithelial proliferation and EMT, which might exert an underlying effect on BPH development and act as therapeutic targets for BPH treatment regimens.

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Decoding ceRNA regulatory network and autophagy-related genes in benign prostatic hyperplasia

Cited by 10 publications

References 56 publications

LncRNA‐associated competing endogenous RNA network analysis uncovered key lncRNAs involved in temozolomide resistance and tumor recurrence of glioblastoma

LncRNA‐associated competing endogenous RNA network analysis uncovered key lncRNAs involved in temozolomide resistance and tumor recurrence of glioblastoma

Epigenetic-related gene-based prognostic model construction and validation in prostate adenocarcinoma

MIR663AHG as a competitive endogenous RNA regulating TGF‐β‐induced epithelial proliferation and epithelial–mesenchymal transition in benign prostate hyperplasia

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