Decoding Acinetobacter baumannii biofilm dynamics and associated protein markers: proteomic and bioinformatics approach

Choudhary, Monika; Kaushik, Shubham; Kapil, Arti; Shrivastava, Rahul; Vashistt, Jitendraa

doi:10.1007/s00203-022-02807-y

Cited by 4 publications

(2 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FepA has been shown to be involved in iron-scavenging system and promote the growth of A. baumannii [30,31]. The tyrosine autokinase is markly elevated to export the exopolysaccharide during biofilm formation [32], and the taurine ABC transporter substrate-binding protein is involved in the transport of taurine as a sulfur source and in competition for taurine utilization between the nosocomial pathogens and host [33].…”

Section: Plos Onementioning

confidence: 99%

Differential proteomic analysis and pathogenic effects of outer membrane vesicles derived from Acinetobacter baumannii under normoxia and hypoxia

et al. 2023

View full text Add to dashboard Cite

Acinetobacter baumannii is a major causative agent of nosocomial infections and its outer membrane vesicles (AbOMVs) have been shown to be involved in pathogenicity by transporting virulence factors and transferring information for communication between pathogens and host cells. Despite the fact that the infected sites of A. baumannii such as lungs and skin soft tissues are hypoxic, most studies on AbOMV virulence have used AbOMVs prepared under aerobic conditions. The present study aims to elucidate the protein profile and pathogenic impact of AbOMVs released under hypoxic condition. AbOMVs were isolated from A. baumannii under normoxic and hypoxic conditions, and their protein profiles were compared. The different effects of both normoxic and hypoxic AbOMVs in cytokine response from mouse macrophages, cytotoxicity to the human lung epithelial cells, and bacterial invasion were then investigated. Our results showed that A. baumannii under hypoxia released larger amounts of OMVs with different protein profiles. Although the cytotoxic effect of AbOMVs from normoxia and hypoxia were comparable, AbOMVs from normoxia induced higher TNF-α production and invasion of Staphylococcus aureus and Pseudomonas aeruginosa than those from hypoxia. On the other hand, AbOMVs significantly enhanced A. baumannii invasion into lung epithelial cells in a dose-dependent manner. These results clearly demonstrate that AbOMVs released from normoxic and hypoxic have different impacts in pathogenesis. This finding provides new insight into the complex interactions between A. baumannii, coinfecting pathogens and host cells via OMVs, in particular the different pathogenic effects of AbOMVs under normoxic and hypoxic conditions.

show abstract

Section: Plos Onementioning

confidence: 99%

Differential proteomic analysis and pathogenic effects of outer membrane vesicles derived from Acinetobacter baumannii under normoxia and hypoxia

et al. 2023

View full text Add to dashboard Cite

show abstract

“…To attempt to explain the microbiological differences observed between Lac-4 and Lac-5, relative protein abundance was monitored. The number of proteins identified in both isolates was similar and corresponded to the average number of proteins identified in previous bacterial studies [108][109][110] . Mass spectrometry based proteome profiling however showed distinct differences between the numbers of identified proteins altered in abundance between the two clinical isolates.…”

Section: Chapter 5: Discussionsupporting

confidence: 82%

Time-course proteomic study of Acinetobacter baumannii after exposure to oxidative stress

Gallo¹

View full text Add to dashboard Cite

Acinetobacter baumannii is an opportunistic nosocomial Gram-negative bacterium responsible for infectious diseases. Due to its rapidly growing multidrug resistance (MDR), there is a need to improve understanding of host-pathogen interactions to identify targets for the development of novel therapeutics. Characterizing bacterial responses to a stress condition representative of a host environment was hypothesized to provide improved understanding of A. baumannii pathogenesis. To this end, an in vitro time-course proteomic study evaluating the impact of oxidative stress on two clinical strains exhibiting different levels of virulence was performed using mass spectrometry. After exposure to hydrogen peroxide, 38 proteins and 181 proteins were observed to have different abundance levels in strain Lac-4 and Lac-5, respectively. The putative localization, pathway and molecular function of these proteins were assessed. Proteins that changed abundance levels were of interest as they indicate potential response to stress and could lead to targets for future therapeutic development.

show abstract

Acinetobacter baumannii Biofilm Formation: Association with Antimicrobial Resistance and Prolonged Survival under Desiccation

2022

View full text Add to dashboard Cite

Decoding Acinetobacter baumannii biofilm dynamics and associated protein markers: proteomic and bioinformatics approach

Cited by 4 publications

References 94 publications

Differential proteomic analysis and pathogenic effects of outer membrane vesicles derived from Acinetobacter baumannii under normoxia and hypoxia

Differential proteomic analysis and pathogenic effects of outer membrane vesicles derived from Acinetobacter baumannii under normoxia and hypoxia

Time-course proteomic study of Acinetobacter baumannii after exposure to oxidative stress

Acinetobacter baumannii Biofilm Formation: Association with Antimicrobial Resistance and Prolonged Survival under Desiccation

Contact Info

Product

Resources

About