Decline of Fibroblast Chemotaxis with Age of Donor and Cell Passage Number

Albini, Adriana; Pontz, B. F.; Pulz, M.; Allavena, G; Mensing, H.; Müller, Peter

doi:10.1016/s0174-173x(88)80033-5

Cited by 42 publications

(12 citation statements)

References 38 publications

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“…Comparable observations were made in assays with human skin fibroblasts which show a migratory response to their own conditioned medium in vitro (Albini et al, 1988;Heckmann et al, 1993;Wach et al, 1998). Mensing and coworkers demonstrated that one of the migration inducing components in the conditioned medium is fibronectin (Mensing et al, 1983).…”

Section: Discussionmentioning

confidence: 94%

“…Human skin fibroblasts have been shown to induce their own migration if exposed to their own conditioned medium in vitro (Albini et al, 1988;Heckmann et al, 1993;Wach et al, 1998). To establish and evaluate our migration assay system with the modified Boyden chamber, conditioned medium of the skin fibroblast cell line NHDFc was tested for its ability to induce migration of primary human CMF ( Fig.…”

Section: Evaluation Of the Migration Assay Systemmentioning

confidence: 99%

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Regulation of Migration of Human Colonic Myofibroblasts

et al. 2002

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Section: Discussionmentioning

confidence: 94%

Section: Evaluation Of the Migration Assay Systemmentioning

confidence: 99%

Regulation of Migration of Human Colonic Myofibroblasts

et al. 2002

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“…Molecular mass labels indicate marker position in the 10% gels. effect of aging on ®broblast migration in both chemotactic and chemokinetic assays (Albini et al, 1988;Pienta and Coffey, 1990) and are particularly relevant to the impact of hypoxia on wound healing since the aged experience a greater incidence of nonhealing wounds associated with low tissue O 2 supply (Eaglstein, 1989;Eaglstein and Falanga, 1997).…”

Section: Discussionmentioning

confidence: 99%

Effect of age and hypoxia on TGFβ1 receptor expression and signal transduction in human dermal fibroblasts: Impact on cell migration

Mogford

Tawil²,

Chen

et al. 2002

Journal Cellular Physiology

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Wound healing is critically affected by age, ischemia, and growth factors such as TGFbeta1. The combined effect of these factors on fibroblast migration, an essential component of wound healing, is poorly understood. To address this deficiency, we examined expression of TGFbeta receptor type I and II (TGFbetaRI and RII) under normoxia or hypoxia (1% O(2)) in cultured human dermal fibroblasts (HDFs) from young (ages 24-33) and aged (ages 61-73) adults. TGFbetaRI and RII expression was similar in both groups under normoxia. Hypoxia did not alter receptor levels in young HDFs but significantly decreased TGFbetaRI in aged cells (12 and 43%, respectively). Additionally, young cells displayed a 50% increase in activation of p42/p44 mitogen-activated kinase by TGFbeta1 (2-200 pg/ml) under hypoxia while aged cell levels of active p42/p44 decreased up to 24%. To determine functional outcomes of these findings, we measured the migratory capacity of the cells on type I collagen using a gold salt migration assay. Hypoxia increased the migratory index (MI) of young HDFs over normoxia by 30% but had no effect on aged cells. Under normoxia, TGFbeta1 (1-1000 pg/ml) increased young HDF migration in a concentration-dependent manner up to 109% over controls but minimally increased aged HDF migration (37%). Under hypoxia, TGFbeta1 significantly increased young cell MI at all concentrations but was without effect on the aged HDF response. These data demonstrate that aged fibroblasts have an impaired migratory capacity with complete loss of responsiveness to hypoxia and deficits in the migratory and signal transduction responsiveness to TGFbeta1 that may partly explain diminished healing capabilities often observed in aged patients.

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“…If the state of differentiation did influence growth factor-dependent aspects of cell behaviour in culture this would in any case have been superimposed on their intentional, or programmed, response. This observation is consistent with the finding reported byAlbini et al (1988) that reduction in the proliferative capacity of fibroblasts is associated with reduced chemotaxis. This only became apparent after P25 in embryo-derived tissue whereas it occurred after P15 in cells from 70-to 90-year-old donors.…”

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confidence: 99%

Motogenic substrata and chemokinetic growth factors for human skin cells

2005

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Extracellular matrix remodelling and accurate spatio-temporal coordination of growth factor expression are two factors that are believed to regulate mitoses and cell migration in developing and regenerating tissues. The present quantitative videomicroscopical study examined the influence of some of the principal components of extracellular matrix and several growth factors that are known to be expressed in dermal wounds on three important facets of human skin cell behaviour in culture. Keratinocytes, melanocytes and dermal fibroblasts (and myofibroblast controls) exhibited varying degrees of substrate adhesion, division and migration depending on the composition of the culture substrate. Substrates that are recognized components of transitional matrices generally accentuated cell adhesion and proliferation, and were motogenic, when compared with serum-treated control surfaces, whereas components of more stable structures such as basement membrane had less influence. Platelet-derived growth factor (PDGF), epidermal growth factor (EGF) and α fibroblastic growth factor ( α FGF) all promoted cell proliferation and were chemokinetic to dermal fibroblasts, but not keratinocyte growth factor (KGF) or transforming growth factor β (TGF β ). PDGF, EGF and KGF, but not TGF β or α FGF, all enhanced proliferation of dermal keratinocytes.The same growth factors, and in addition KGF, all stimulated motility in keratinocytes, but TGF β and α FGF again had no effect. Developing a better understanding of the interdependency of factors that control crucial cell behaviour may assist those who are interested in the regulation of histogenesis and also inform the development of rational therapeutic strategies for the management of chronic and poorly healed wounds.

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Decline of Fibroblast Chemotaxis with Age of Donor and Cell Passage Number

Cited by 42 publications

References 38 publications

Regulation of Migration of Human Colonic Myofibroblasts

Regulation of Migration of Human Colonic Myofibroblasts

Effect of age and hypoxia on TGFβ1 receptor expression and signal transduction in human dermal fibroblasts: Impact on cell migration

Motogenic substrata and chemokinetic growth factors for human skin cells

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