Deciphering the vascular labyrinth: role of microRNAs and candidate gene SNPs in brain AVM development – literature review

Florian, Ioan Alexandru; Ungureanu, Gheorghe; Bălaşa, A; Berindan‐Neagoe, Ioana

doi:10.1080/01616412.2020.1796380

Cited by 10 publications

(5 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous study ( Wu et al 2022 ), we searched the target genes of miR-138-5p by using established miRNA target prediction programs and identified GPR124 as a potential target of miR-138-5p using TargetScan. GPR124 is an orphan G protein-coupled receptor that functionally modulates physiological angiogenesis and embryonic alteration within the central nervous system ( Erkinova et al 2018 , Florian et al 2020 ). The physiological role of GPR124 is established in angiogenesis and atherosclerosis potentially associated with embryo implantation.…”

Section: Discussionmentioning

confidence: 99%

LIF–STAT signaling in decidual cells: a possible role in embryo implantation and early pregnancy

Wu,

Chen,

Chiu

et al. 2024

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

In this study, we investigate the effects of microRNA-138-5p and GPR124-regulated inflammasome and downstream LIF-STAT and adhesion molecules signaling in human decidual stromal cells. After informed consent was obtained from women aged 25-38 years undergoing surgical termination of the normal pregnancy and spontaneous miscarriage after 6-9 weeks of gestation, human decidual stromal cells were separated from the decidual tissue. Extracellular vesicles with microRNA (miRNA) between cells have been regarded as critical factors for embryo-maternal interactions on embryo implantation and programming of human pregnancy. MicroRNA-138-5p acts as the transcriptional regulator of GPR124 and the mediator of downstream inflammasome. LIF-regulated STAT activation and expression of integrins might influence embryo implantation. Hence, a better understanding of LIF-STAT and adhesion molecules signaling would elucidate the mechanism of microRNA-138-5p- and GPR124-regulated inflammasome activation on embryo implantation and pregnancy. Our results show the purified extracellular vesicles from decidual stromal cells, the microRNA-138-5p-inhibited GPR124 and inflammasome expression, and microRNA-138-5p activated the expression of LIF-STAT and adhesion molecules in human decidual stromal cells. Additionally, the knockdown of GPR124 and NLRP3 through siRNA increases the expression of LIF-STAT and adhesion molecules. Our findings reveal a better understanding the role of extracellular vesicles, microRNA-138-5p, GPR124, inflammasome, and LIF-STAT and adhesion molecules in embryo implantation and programming of human pregnancy.

show abstract

Section: Discussionmentioning

confidence: 99%

LIF–STAT signaling in decidual cells: a possible role in embryo implantation and early pregnancy

Wu,

Chen,

Chiu

et al. 2024

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

show abstract

“…GPR124 was predicted as a potential target of miR-138-5p by Target scan. GPR124 functionally promotes physiological embryonic and angiogenesis within the central nervous system [26,27]. We focused on GPR124 because of its established relevance in angiogenesis and atherosclerosis possibly related to embryo implantation.…”

Section: Discussionmentioning

confidence: 99%

Extracellular Vesicle-Associated MicroRNA-138-5p Regulates Embryo Implantation and Early Pregnancy by Adjusting GPR124

Tsai

et al. 2022

Pharmaceutics

View full text Add to dashboard Cite

Functional embryo–maternal interactions occur during the embryo implantation and placentation. Extracellular vesicles with microRNA (miR) between cells have been considered of critical importance for embryo implantation and the programming of human pregnancy. MiR-138-5p functions as the transcriptional regulator of G protein-coupled receptor 124 (GPR124). However, the signaling pathway of miR138-5p- and GPR124-adjusted NLRP3 inflammasome activation remains unclear. In this study, we examine the roles of the miR138-5p and GPR124-regulated inflammasome in embryo implantation and early pregnancy. Human decidual stromal cells were isolated from the abortus tissue and collected by curettage from missed abortion patients and normal pregnant women at 6– to 12-week gestation, after informed consent. Isolated extracellular vesicles from decidua and decidual stromal cells were confirmed by transmission electron microscopy (TEM). Next-Generation Sequencing (NGS) and microarray were performed for miR analysis. The predicated target genes of the differentially expressed miR were analyzed to identify the target genes and their pathway. We demonstrated the down-regulation of miR-138-5p and the overexpression of GPR124 in spontaneous miscarriage compared to normal pregnancy. We also showed the excessive activation of the NLRP3 inflammasome in spontaneous miscarriage compared to normal pregnancy. Here, we newly demonstrate that the miR-138-5p and GPR124-adjusted NLRP3 inflammasome were expressed in extracellular vesicles derived from decidua and decidual stromal cells, indicating that the miR-138-5p, GPR124 and NLRP3 (NACHT, LRR, and PYD domains-containing protein 3) inflammasome have a potential modulatory role on the decidual programming and placentation of human pregnancy. Our findings represent a new concept regarding the role of extracellular vesicles, miR-138-5p, GPR124, and the NLRP3 inflammasome in normal early pregnancy and spontaneous miscarriage.

show abstract

“…GPR124 (probable G-protein coupled receptor 124) was predicted as a potential target of miR-138-5p by Target scan. GPR124 is an orphan G protein-coupled receptor that functionally promotes physiological embryonic and angiogenesis within the central nervous system [24,25]. We focused on GPR124 because of its established relevance in angiogenesis and atherosclerosis possibly related to embryo implantation.…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicle-associated microRNA-138-5p regulates embryo implantation and early pregnancy by adjusting GPR124

Tsai

et al. 2022

Preprint

View full text Add to dashboard Cite

Background Functional embryo-maternal interactions occur during the embryo implantation and placentation. Extracellular vesicles with microRNA (miRNA) between cells have been considered of critical importance for embryo implantation and programming of human pregnancy. MicroRNA-138-5p functions as the transcriptional regulator of GPR124. However, the mechanism of microRNA-138-5p- and GPR124-regulated NLRP3 inflammasome activation remains unclear. In this study, we examine the roles of microRNA-138-5p and GPR124-regulated inflammasome in embryo implantation and early pregnancy. Methods Human decidual stromal cells were isolated from the abortus tissue, collected by curettage from missed abortion patients and normal pregnant women at 6- to 12-wk gestation, after informed consent. Transmission electron microscopy (TEM) images of isolated extracellular vesicles were obtained. NGS and microarray were performed for miRNAs analysis. The predicated target genes of the differentially expressed miRNA were analyzed to identify the target genes and their pathway. Results Isolated extracellular vesicles from decidua and decidual stromal cells were confirmed by TEM images. We demonstrated the down-regulation of miR-138-5p and the overexpression of GPR124 in spontaneous miscarriage compared to normal pregnancy. We also showed the excessive activation of NLRP3 inflammasome in spontaneous miscarriage compared to normal pregnancy. Conclusions Here we newly demonstrate that the miR-138-5p and GPR124-regulated NLRP3 inflammasome were expressed in extracellular vesicles derived from decidua and decidual stromal cells, indicating the miR-138-5p, GPR124 and NLRP3 inflammasome have potential modulatory role on decidual programming and placentation of human pregnancy. Our findings represent a new concept regarding the role of extracellular vesicles, miR-138-5p, GPR124, and NLRP3 inflammasome in normal early pregnancy and spontaneous miscarriage.

show abstract

Deciphering the vascular labyrinth: role of microRNAs and candidate gene SNPs in brain AVM development – literature review

Cited by 10 publications

References 70 publications

LIF–STAT signaling in decidual cells: a possible role in embryo implantation and early pregnancy

LIF–STAT signaling in decidual cells: a possible role in embryo implantation and early pregnancy

Extracellular Vesicle-Associated MicroRNA-138-5p Regulates Embryo Implantation and Early Pregnancy by Adjusting GPR124

Extracellular vesicle-associated microRNA-138-5p regulates embryo implantation and early pregnancy by adjusting GPR124

Contact Info

Product

Resources

About