Deciphering the transcriptomic response of Fusarium verticillioides in relation to nitrogen availability and the development of sugarcane pokkah boeng disease

Lin, Zhenping; Wang, Jihua; Bao, Yixue; Guo, Qiang; Powell, Charles A.; Xu, Shiqiang; Chen, Baoshan

doi:10.1038/srep29692

Cited by 26 publications

(19 citation statements)

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“…7 ). Our results suggested that chronic eutrophication influenced multiple specific biological processes by altering transcription accumulation 40 and coordinating the expression of two aspects of the coral host and Symbiodinium symbionts. However, the mechanisms of global transcriptional changes and their physiological consequences are not yet fully understood.…”

Section: Discussionmentioning

confidence: 78%

Transcriptome profiling of Galaxea fascicularis and its endosymbiont Symbiodinium reveals chronic eutrophication tolerance pathways and metabolic mutualism between partners

Lin

Chen

et al. 2017

Sci Rep

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In the South China Sea, coastal eutrophication in the Beibu Gulf has seriously threatened reef habitats by subjecting corals to chronic physiological stress. To determine how coral holobionts may tolerate such conditions, we examined the transcriptomes of healthy colonies of the galaxy coral Galaxea fascicularis and its endosymbiont Symbiodinium from two reef sites experiencing pristine or eutrophied nutrient regimes. We identified 236 and 205 genes that were differentially expressed in eutrophied hosts and symbionts, respectively. Both gene sets included pathways related to stress responses and metabolic interactions. An analysis of genes originating from each partner revealed striking metabolic integration with respect to vitamins, cofactors, amino acids, fatty acids, and secondary metabolite biosynthesis. The expression levels of these genes supported the existence of a continuum of mutualism in this coral-algal symbiosis. Additionally, large sets of transcription factors, cell signal transduction molecules, biomineralization components, and galaxin-related proteins were expanded in G. fascicularis relative to other coral species.

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Section: Discussionmentioning

confidence: 78%

Transcriptome profiling of Galaxea fascicularis and its endosymbiont Symbiodinium reveals chronic eutrophication tolerance pathways and metabolic mutualism between partners

Lin

Chen

et al. 2017

Sci Rep

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“…A 2 -ΔΔCt algorithm was used to evaluate the relative fold change in the expression of the each gene using the act1 (actin) gene as an endogenous control. [58, 59] The data were analyzed using LightCycler® 480 software version 1.5.1 (Roche). Pearson’s correlation coefficients were calculated to evaluate the correlation of gene expression obtained by RNA-seq and qRT-PCR using Origin 9.0 software (Origin Lab).…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of carbendazim resistance of Fusarium species complex that causes sugarcane pokkah boeng disease

Wang

et al. 2019

BMC Genomics

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BackgroundPokkah boeng is one of the most serious and devastating diseases of sugarcane and causes significant loss in cane yield and sugar content. Although carbendazim is widely used to prevent fungal diseases, the molecular basis of Fusarium species complex (FSC) resistance to carbendazim remains unknown.ResultsThe EC50 (fungicide concentration that inhibits 50% of mycelial growth) values of carbendazim for 35 FSC isolates collected in cane growing regions of China were ranged from 0.5097 to 0.6941 μg mL− 1 of active ingredient (a.i.), in an average of 0.5957 μg a.i. mL− 1. Among carbendazim-induced mutant strains, SJ51M (F. verticillioides) had a CTG rather than CAG codon (Q134L) at position 134 of the FVER_09254 gene, whereas in the mutant strain HC30M (F. proliferatum) codon ACA at position 351 of the FPRO_07779 gene was replaced by ATA (T351I). Gene expression profiling analysis was performed for SJ51M and its corresponding wild type strain SJ51, with and without carbendazim treatment. The gene expression patterns in SJ51 and SJ51M changed greatly as evidenced by the detection of 850 differentially expressed genes (DEGs). Functional categorization indicated that genes associated with oxidation-reduction process, ATP binding, integral component of membrane, transmembrane transport and response to stress showed the largest expression changes between SJ51M and SJ51. The expression levels of many genes involved in fungicide resistance, such as detoxification enzymes, drug efflux transporters and response to stress, were up-regulated in SJ51M compared to SJ51 with and without carbendazim treatment.ConclusionFSC was sensitive to carbendazim and had the potential for rapid development of carbendazim resistance. The transcriptome data provided insight into the molecular pathways involved in FSC carbendazim resistance.Electronic supplementary materialThe online version of this article (10.1186/s12864-019-5479-6) contains supplementary material, which is available to authorized users.

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“…In particular, Fusarium verticillioides is a phytopathogenic fungus of economic importance because it is the most commonly reported fungal species that infects maize (32), in addition to causing stalk rot disease in sorghum (33) and Pokkah Boeng disease in sugarcane (34). The diseases caused by Fusarium spp.…”

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confidence: 99%

Stress-Responsive Alternative Sigma Factor SigB Plays a Positive Role in the Antifungal Proficiency of Bacillus subtilis

Bartolini

Cogliati

Vileta

et al. 2019

Appl Environ Microbiol

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DifferentBacillusspecies with PGPR (plant growth-promoting rhizobacterium) activity produce potent biofungicides and stimulate plant defense responses against phytopathogenic fungi. However, very little is known about how these PGPRs recognize phytopathogens and exhibit the antifungal response. Here, we report the antagonistic interaction betweenBacillus subtilisand the phytopathogenic fungusFusarium verticillioides. We demonstrate that this bacterial-fungal interaction triggers the induction of the SigB transcription factor, the master regulator ofB. subtilisstress adaptation. Dual-growth experiments performed with live or dead mycelia or culture supernatants ofF. verticillioidesshowed that SigB was activated and required for the biocontrol of fungal growth. Mutations in the different regulatory pathways of SigB activation in the isogenic background revealed that only the energy-related RsbP-dependent arm of SigB activation was responsible for specific fungal detection and triggering the antagonistic response. The activation of SigB increased the expression of the operon responsible for the production of the antimicrobial cyclic lipopeptide surfactin (thesrfAoperon). SigB-deficientB. subtiliscultures produced decreased amounts of surfactin, andB. subtiliscultures defective in surfactin production (ΔsrfA) were unable to control the growth ofF. verticillioides.In vivoexperiments of seed germination efficiency and early plant growth inhibition in the presence ofF. verticillioidesconfirmed the physiological importance of SigB activity for plant bioprotection.IMPORTANCEBiological control using beneficial bacteria (PGPRs) represents an attractive and environment-friendly alternative to pesticides for controlling plant diseases. Different PGPRBacillusspecies produce potent biofungicides and stimulate plant defense responses against phytopathogenic fungi. However, very little is known about how PGPRs recognize phytopathogens and process the antifungal response. Here, we report howB. subtilistriggers the induction of the stress-responsive sigma B transcription factor and the synthesis of the lipopeptide surfactin to fight the phytopathogen. Our findings show the participation of the stress-responsive regulon of PGPRBacillusin the detection and biocontrol of a phytopathogenic fungus of agronomic impact.

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Deciphering the transcriptomic response of Fusarium verticillioides in relation to nitrogen availability and the development of sugarcane pokkah boeng disease

Cited by 26 publications

References 59 publications

Transcriptome profiling of Galaxea fascicularis and its endosymbiont Symbiodinium reveals chronic eutrophication tolerance pathways and metabolic mutualism between partners

Transcriptome profiling of Galaxea fascicularis and its endosymbiont Symbiodinium reveals chronic eutrophication tolerance pathways and metabolic mutualism between partners

Molecular characterization of carbendazim resistance of Fusarium species complex that causes sugarcane pokkah boeng disease

Stress-Responsive Alternative Sigma Factor SigB Plays a Positive Role in the Antifungal Proficiency of Bacillus subtilis

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