2013
DOI: 10.1016/j.ibmb.2013.07.007
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Deciphering the synergism of endogenous glycoside hydrolase families 1 and 9 from Coptotermes gestroi

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Cited by 28 publications
(31 citation statements)
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“…To further examine the ability of this cellulase to breakdown insoluble complex carbohydrates with industrial interest, hydrolytic activity was investigated using Avicel and BEX as substrates. The rate of sugar released using Avicel was 2- and 8-fold higher than obtained for cellulases Cel5A from Gloeophyllum trabeum and endoglucanase CgEG1 from Coptotermes gestroi , which were 4.5 x 10 -3 and 1.1 x 10 -3 U.mg -1 , respectively [30,31]. The glycoside hydrolase CelE1 was also active on BEX (Table 2).…”
Section: Resultsmentioning
confidence: 99%
“…To further examine the ability of this cellulase to breakdown insoluble complex carbohydrates with industrial interest, hydrolytic activity was investigated using Avicel and BEX as substrates. The rate of sugar released using Avicel was 2- and 8-fold higher than obtained for cellulases Cel5A from Gloeophyllum trabeum and endoglucanase CgEG1 from Coptotermes gestroi , which were 4.5 x 10 -3 and 1.1 x 10 -3 U.mg -1 , respectively [30,31]. The glycoside hydrolase CelE1 was also active on BEX (Table 2).…”
Section: Resultsmentioning
confidence: 99%
“…It has been reported that CgEG1 and CgBG1 from the termite C. gestroi show synergistic effect in enzyme activity toward glucose oligomers [18]. In addition, synergism study on RfEG1 and RfBG1 from the termite Reticulitermes flavipes against microcrystalline cellulose was also reported [33].…”
Section: Discussionmentioning
confidence: 97%
“…Next, we performed assays combining Cg AKR-1, Cg GH9 ( C. gestroi endoglucanase [56]), and CAT (a commercial catalase that catalyzes the decomposition of H 2 O 2 to water and oxygen) in order to evaluate whether the synergism of these enzymes on barley beta-glucan saccharification could be correlated with ROS generation. Hydrolysis with Cg GH9 released reducing sugars and background H 2 O 2 production (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…The sequence encoding full-length CGAKR1 was amplified from C. gestroi cDNA using a standard polymerase chain reaction (PCR) method, as previously described [56]. Two nucleotide primers were used, as follows: (forward, 5′-TAAAAT GCTAGC ATGCCTAAACAACTGAGCAGT-3′, and reverse, 5′-TATTAT GGATCC CTAATAAGGCTCATCATACGGGT-3′; restriction enzyme recognition sites are underlined).…”
Section: Methodsmentioning
confidence: 99%
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