Deciphering the Synergies of Reductive Soil Disinfestation Combined with Biochar and Antagonistic Microbial Inoculation in Cucumber Fusarium Wilt Suppression Through Rhizosphere Microbiota Structure

Ali, Ahmad; Elrys, Ahmed S.; Liu, Liangliang; Xia, Qiuyuan; Wang, Baoying; Li, Yunlong; Dan, Xiaoqian; Iqbal, Muhammad; Zhao, Jun; Huang, Xinqi; Cai, Zucong

doi:10.1007/s00248-022-02097-3

Cited by 11 publications

(4 citation statements)

References 59 publications

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“…Specifically, RSD-completed soil combined with the inoculation of beneficial species (such as B. subtilis SQR-N1 and Trichoderma spp.) has attracted increasing attention for enhancing plant disease resistance; this is mainly because RSD alone cannot always perform well to control soil-borne diseases during plant cultivation ( Huang et al., 2016 ; Khadka and Miller, 2021 ; Ali et al., 2022 ). For example, our previous study showed that the control efficiencies of RSD on Fusarium wilt and F. oxysporum in watermelon were unsatisfactory ( Liu et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…Herein, for example, the specific bacterial taxa (e.g, Bacillus , Cohnella , and Paenibacillus spp.) have been previously reported as the potential PGRPs, which have the capacity to promote plant growth by establishing defense lines against pathogen invasion in the rhizosphere ( Li and Chen, 2019 ; Premalatha et al., 2021 ; Ali et al., 2022 ; Zhou et al., 2022 ). The species of Oxobacter , Rummeliibacillus , and Thermoanaerobacterium can suppress pathogens by releasing organic acids such as acetic acid, ethanol, and lactic acid during the fermentation of organic materials ( Mowlick et al., 2013 ; Pang et al., 2018 ; Tan et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is important to develop appropriate methods to promote the colonization efficiency of these native functional microorganisms, especially facultative anaerobic bacteria, in RSD-treated soils for maintaining soil and plant health. Our previous studies showed that biocontrol agents such as Bacillus subtilis SQR-N1 and Trichoderma harzianum SQR-T37 inoculated into the RSD-completed soil (aerobic conditions) could further enhance its resistance to damping-off and Fusarium wilt diseases of cucumber ( Huang et al., 2016 ; Ali et al., 2022 ). However, we are unsure whether the functional microorganisms inoculated into the strongly anaerobic and reductive environment, which is the RSD treatment process, may improve their colonization efficiencies and plant disease resistance.…”

Section: Introductionmentioning

confidence: 99%

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Facilitating effects of the reductive soil disinfestation process combined with Paenibacillus sp. amendment on soil health and physiological properties of Momordica charantia

et al. 2023

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Reductive soil disinfestation (RSD) is an anaerobic and facultative anaerobic microbial-mediated soil management process. The extent of improvement of diseased soil properties by RSD relative to comparable healthy soil is, however, not well characterized. Importantly, how to promote the colonization efficiency of these facultative anaerobic functional species to ensure soil and plant health remain unknown. Here, Fusarium wilt-diseased soil of Momordica charantia grown under a plastic-shed field (PS-CK) was used to conduct molasses-RSD (MO-RSD) along with Paenibacillus sp. (a model of facultative anaerobic species) (MOPA-RSD) treatment, and the soil from a nearby open-air paddy field was considered comparable healthy soil (OA-CK). Both RSD treatments significantly improved the properties of PS-CK soil, and the extent of improvement of soil pH, Fusarium oxysporum reduction efficiency (98.36%~99.56%), and microbial community and functional composition were higher than that achieved for OA-CK soil, which indicated that RSD-regulated most soil properties outperformed those of the comparable healthy soil. The disease incidence and ascorbic acid content of M. charantia in MO-RSD- and MOPA-RSD-treated soils were considerably decreased, while the weight and soluble protein contents were correspondingly increased, as compared to those of M. charantia in PS-CK soil. Specifically, the changes in these physiological properties of M. charantia in MOPA-RSD soil performed well than that in MO-RSD soil. The relative abundances of Cohnella, Effusibacillus, Rummeliibacillus, Oxobacter, Thermicanus, and Penicillium enriched in both RSD-treated soils were positively correlated with Paenibacillus and negatively correlated with F. oxysporum population and disease incidence (P < 0.05). Notably, the relative abundances of these potential probiotics were considerably higher in MOPA-RSD-treated soil than in MO-RSD alone-treated soil. These results show that the RSD process with inoculation of Paenibacillus sp. could promote the colonization of this species and simultaneously stimulate the proliferation of other probiotic consortia to further enhance soil health and plant disease resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Facilitating effects of the reductive soil disinfestation process combined with Paenibacillus sp. amendment on soil health and physiological properties of Momordica charantia

et al. 2023

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“…Zhao et al (2020) revealed that diversity and decomposability of organic materials added in RSD may determine the extent of microbial activity improvement. Meanwhile, soil abiotic factors, such as pH and carbon content, can also highly influence the dissimilarity and assembly of soil microbial communities in RSD-treated soil (Liu et al, 2019(Liu et al, , 2022Ali et al, 2022). Besides, a growing number of studies suggest that RSD-regulated soil microbial communities may deteriorate to a state similar to those of diseased soils after crop cultivation, which might be affected by root exudates (Mowlick et al, 2013;Liu et al, 2018;Huang et al, 2019a).…”

Section: Introductionmentioning

confidence: 99%

Contributions of carbon source, crop cultivation, and chemical property on microbial community assemblage in soil subjected to reductive disinfestation

Zhu

Lü²,

Hong³

et al. 2023

Front. Microbiol.

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In agricultural practice, reductive soil disinfestation (RSD) is an effective method for eliminating soil-borne pathogens that depends heavily on carbon source. However, knowledge regarding the assembly of soil microbial communities in RDS-treated soils amended with different carbon sources after continuous crop cultivation is still not well-characterized. RSD treatments were performed on greenhouse soil with six different carbon sources (ethanol, glucose, alfalfa, wheat bran, rice bran, and sugarcane residue), which have different C:N ratios (Org C/N) and easily oxidized carbon contents (Org EOC). After RSD, two consecutive seasons of pepper pot experiments were conducted. Then, the effects of carbon source property, crop cultivation, and soil chemical property on soil microbial community reestablishment, pathogen reproduction, and crop performance were investigated in the RSD-cropping system. Variation partition analysis indicated that carbon source property, crop cultivation, and soil chemical property explained 66.2 and 39.0% of bacterial and fungal community variation, respectively. Specifically, Mantel tests showed that Org C/N, crop cultivation, soil available phosphorus and potassium were the most important factors shaping bacterial community composition, while Org C/N, Org EOC, and crop cultivation were the most important factors shaping fungal community composition. After two planting seasons, the number of cultivable Fusarium was positively correlated with Org EOC, and negatively correlated with soil total organic carbon, Fungal Chao1, and Fungal PC1. Crop yield of complex-carbon soils (Al, Wh, Ri and Su) was negatively affected by Org C/N after the first season, and it was highest in Al, and lower in Et and Su after the second season. Overall, Org EOC and Org C/N of carbon source were vitally important for soil microbe reestablishment, Fusarium reproduction and crop performance. Our findings further broaden the important role of carbon source in the RSD-cropping system, and provide a theoretical basis for organic carbon selection in RSD practice.

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Role of Fungi in Imparting General Disease Suppressiveness in Soil from Organic Field

et al. 2023

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Deciphering the Synergies of Reductive Soil Disinfestation Combined with Biochar and Antagonistic Microbial Inoculation in Cucumber Fusarium Wilt Suppression Through Rhizosphere Microbiota Structure

Cited by 11 publications

References 59 publications

Facilitating effects of the reductive soil disinfestation process combined with Paenibacillus sp. amendment on soil health and physiological properties of Momordica charantia

Facilitating effects of the reductive soil disinfestation process combined with Paenibacillus sp. amendment on soil health and physiological properties of Momordica charantia

Contributions of carbon source, crop cultivation, and chemical property on microbial community assemblage in soil subjected to reductive disinfestation

Role of Fungi in Imparting General Disease Suppressiveness in Soil from Organic Field

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