Deciphering the roles of miR-16-5p in malignant solid tumors

Yang, Liuyi; Yang, Sen; Ren, Congcong; Liu, Shihua; Zhang, Xiaopei; Sui, Aixia

doi:10.1016/j.biopha.2022.112703

Cited by 13 publications

(9 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 12,27,28 ] The ubiquitously expressed miR‐16 , on the other hand, participates in many biological processes and is one of the most abundant circulating miRNAs. [ 29 ] We detected both miRNAs in the attached exosomal fraction (Figure 5C). Interestingly, bound miR‐122 represented a higher fraction of the total miR‐122 present in the input sample than was the case for bound miR‐16 concerning its own input (Figure 5C).…”

Section: Resultsmentioning

confidence: 99%

Immunoaffinity‐Based Microfluidic Platform for Exosomal MicroRNA Isolation from Obese and Lean Mouse Plasma

Rodríguez‐Comas

Castaño

Ortega

et al. 2023

Adv Materials Technologies

View full text Add to dashboard Cite

A new way of intercellular communication has been recently demonstrated, consisting of the controlled release of small vesicles loaded with bioactive factors (proteins, lipids, and nucleic acids chiefly amongst them) to the extracellular compartment. Importantly, these extracellular vesicles (EVs), including microvesicles and exosomes, regulate various physiological processes and, when disturbed, may contribute to the onset and progression of many diseases. Hence, they can be exploited as informative biomarkers to diagnose and monitor pathological conditions, including type 2 diabetes and associated metabolic disease. However, currently available techniques to isolate EVs lack reproducibility, effectiveness, and rapidity and require high amounts of starting samples, limiting their introduction in clinical practice. The objective of this work is to use a simple and easy‐to‐use microfluidic platform for capturing and quantifying a specific subpopulation of EVs from a small volume of plasma. As a proof‐of‐concept, the platform is tested with murine plasma. An analysis of the microRNA (miRNA) content of the isolated EVs is performed, detecting significant differences when comparing lean and obese mice. Thus, the device offers an easy, straightforward system to detect and analyze plasma‐derived EVs in the everyday clinical setting.

show abstract

Section: Resultsmentioning

confidence: 99%

Immunoaffinity‐Based Microfluidic Platform for Exosomal MicroRNA Isolation from Obese and Lean Mouse Plasma

Rodríguez‐Comas

Castaño

Ortega

et al. 2023

Adv Materials Technologies

View full text Add to dashboard Cite

show abstract

“…Other studies showed at least seven host miRNAs (has-miRNA-(15a, 298, 497, 508, 1909, and 3130) targeting SARS-CoV-2-S glycoprotein at various locations, leading to substantial inhibition of the virus replication (39). The candidates of the host miRNA-16 family are involved in many cellular processes, including cell cycle arrest, tumor research, and immune regulation, particularly the TGFβ pathway, and they are also used as diagnostic and prognostic markers for cancer research (40,41,42,43,44,45). Recent studies showed that the host miRNA-16-2-3p could be an excellent prognostic marker for SARS-CoV-2 infection in humans (46).…”

Section: -Discussionmentioning

confidence: 99%

The dual actions of the host miRNA-16a in restricting Bovine coronavirus (BCoV) replication through targeting host cell Furin and in enhancing the host immune response

Shah,

Hemida

2024

Preprint

View full text Add to dashboard Cite

The roles of host cell miRNAs have not been studied well in the context of BCoV replication and immune regulation. The main aim of this study was to identify some miRNA candidates that regulate essential host genes involved in BCoV replication, tissue tropism, and immune regulation. To achieve these goals, we used two isolates of BCoV (enteric and respiratory) to infect the bovine endothelial cells (BEC) and Madine Darby Bovine Kidney (MDBK) cells. This is in addition to the ex vivo model using the peripheral bovine blood mononuclear cells (PBMC). We determined the miRNA expression profiles in these cells after BCoV infection. miRA-16a is one of the differentially altered during BCoV infection. Our data shows that miRNA-16a is a significantly downregulated miRNA in both in vitro and ex vivo models. We confirmed the miRNA-16a expression profile by the qRT-PCR. Overexpression of the pre-miRNA-16a in BEC and MDBK cell lines resulted in marked inhibition of BCoV infection based on the viral genome copy numbers measured by qRT-PCR, the viral protein expression (S and N) measured by Western blot, and the virus infectivity using plaque assay. Our bioinformatic prediction showed that Furin is a potential target for the miRNA-16a. We checked the Furin protein expression level in the pre-miRNA-16a transfected/BCoV infected cells compared to the pre-miRNA scrambled to validate that. Our data shows marked inhibition of the Furin expression levels on the mRNA levels by qRT-PCR and the protein level by Western blot. The BCoV-S protein expression was markedly inhibited on both the mRNA and protein levels. To further confirm the impacts of the downregulation of the Furin enzyme on the replication of BCoV, we used transfected cells with specific Furin-siRNA parallel to the scrambled siRNA. A marked inhibition of BCoV replication was observed in the Furin-siRNA-treated group. To further validate Furin as a novel target for miRNA-16a, we cloned the 3'UTR of the bovine Furin carrying the seed region of the miRNA-16a in the dual luciferase vector. Our data shows luciferase activity in the pre-miRNA-16a transfected cells decreased by more than 50% compared to the cells transfected with the construct carrying the mutated Furin seed region. Our data confirms miRNA-16a inhibits BCoV replication by targeting the host cell Furin and the BCoV-S glycoprotein. It will also enhance the host immune response, which contributes to the inhibition of viral replication. To our knowledge, this is the first study to confirm that Furin is a valid target for the miRNA-16a. Our findings highlight the clinical applications of the host miRNA-16a as a potential miRNA-based vaccine/antiviral therapy.

show abstract

“…Other studies have shown that at least seven host miRNAs (hsa-miRNA-(15a, 298, 497, 508, 1909, and 3130)) target the SARS-CoV-2-S glycoprotein at various locations, leading to substantial inhibition of virus replication (39). The candidates of the host miRNA-16 family are involved in many cellular processes, including cell cycle arrest, tumor research, and immune regulation, particularly the TGFβ pathway, and they are also used as diagnostic and prognostic markers for cancer research (40,41,42,43,44,45). Recent studies have shown that host miRNA-16-2-3p could be an excellent prognostic marker for SARS-CoV-2 infection in humans (46).…”

Section: Discussionmentioning

confidence: 99%

The dual actions of host miRNA-16a in restricting bovine coronavirus (BCoV) replication through targeting the host cell Furin and enhancing the host immune response

Shah,

Hemida

2024

Preprint

View full text Add to dashboard Cite

The roles of host cell miRNAs have not been well studied in the context of BCoV replication and immune regulation. The main aim of this study was to identify miRNA candidates that regulate essential host genes involved in BCoV replication, tissue tropism, and immune regulation. To achieve these goals, we used two isolates of BCoV (enteric and respiratory) to infect bovine endothelial cells (BECs) and Madine Darby Bovine Kidney (MDBK) cells. This is in addition to the ex vivo model using peripheral bovine blood mononuclear cells (PBMCs). We determined the miRNA expression profiles of these cells after BCoV infection. The expression of miR-16a is differentially altered during BCoV infection. Our data show that miRNA-16a is a significantly downregulated miRNA in both in vitro and ex vivo models. We confirmed the miRNA-16a expression profile by qRT‒PCR. Overexpression of pre-miRNA-16a in the BEC and MDBK cell lines markedly inhibited BCoV infection, as determined by the viral genome copy numbers measured by qRT‒PCR, viral protein expression (S and N) measured by Western blot, and virus infectivity using a plaque assay. Our bioinformatic prediction showed that Furin is a potential target of miRNA-16a. We compared the Furin protein expression level in pre-miRNA-16a-transfected/BCoV-infected cells to that in pre-miRNA-scrambled-transfected cells. Our qRT‒PCR and Western blot data revealed marked inhibition of Furin expression at the mRNA level and at the protein level, respectively. BCoV-S protein expression was markedly inhibited at both the mRNA and protein levels. To further confirm the impact of the downregulation of the Furin enzyme on the replication of BCoV, we transfected cells with specific Furin-siRNAs parallel to the scrambled siRNA. Marked inhibition of BCoV replication was observed in the Furin-siRNA-treated group. To further validate Furin as a novel target for miRNA-16a, we cloned the 3'UTR of bovine Furin carrying the seed region of miRNA-16a in a dual luciferase vector. Our data showed that luciferase activity in pre-miRNA-16a-transfected cells decreased by more than 50% compared to that in cells transfected with the construct carrying the mutated Furin seed region. Our data confirmed that miRNA-16a inhibits BCoV replication by targeting the host cell line Furin and the BCoV-S glycoprotein. It also enhances the host immune response, which contributes to the inhibition of viral replication. To our knowledge, this is the first study to confirm that Furin is a valid target of miRNA-16a. Our findings highlight the clinical applications of host miRNA-16a as a potential miRNA-based vaccine/antiviral therapy.

show abstract

Deciphering the roles of miR-16-5p in malignant solid tumors

Cited by 13 publications

References 97 publications

Immunoaffinity‐Based Microfluidic Platform for Exosomal MicroRNA Isolation from Obese and Lean Mouse Plasma

Immunoaffinity‐Based Microfluidic Platform for Exosomal MicroRNA Isolation from Obese and Lean Mouse Plasma

The dual actions of the host miRNA-16a in restricting Bovine coronavirus (BCoV) replication through targeting host cell Furin and in enhancing the host immune response

The dual actions of host miRNA-16a in restricting bovine coronavirus (BCoV) replication through targeting the host cell Furin and enhancing the host immune response

Contact Info

Product

Resources

About