Deciphering the role of SPL12 and AGL6 from a genetic module that functions in nodulation and root regeneration in Medicago sativa

Nasrollahi, Vida; Yuan, Zhongshun; Lü, Qing; McDowell, Tim; Kohalmi, Susanne E.; Hannoufa, Abdelali

doi:10.1007/s11103-022-01303-7

Cited by 6 publications

(16 citation statements)

References 113 publications

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“…Interestingly, our analysis returned additional MADS box members with above threshold hits which include FLC, SVP, AP1, AGL6, AGL15, FUL, AG, SEP1, and AGL24. Such occurrence is supported by other studies like that in sweet potato for SVP, and AGL24 [42], Medicago sativa for AGL6 [43] and AP1 [44], Arabidopsis for FLC [45] and AGL15 [46] etc.…”

Section: Rootssupporting

confidence: 80%

Functional Overview on Mads Box: A Meta-Review

Adhikari,

Kasahara

2024

Preprint

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Majority of the studied MADS box members are linked with flowering and fruit traits. However, higher volumes of studies on the type II of their two types so far suggest that florigenic effect of the gene members could just be tip of the iceberg. In current study we used a systematic approach to have general overview on the MADS box members their cross-trait and multifactor associations as well as their pleiotropic potentials based on local reference database curated for MADS box members. While doing so, we screened for the co-occurrence of the terms of interest within the title or abstract of each reference with threshold of 3 hits. Analysis results showed that our approach can retrieve multi-faceted information on the subject of study (MADS box gene members in current case) which could otherwise have been skewed depending on the authors’ expertise and/or volume of literature reference base. Overall, our study discusses on the roles of MADS box members in association with plant organs and traits-linked factors among plant species. Our assessment showed that plants with majority of the MADS box member studies include tomato, apple, rice etc., after Arabidopsis. Furthermore, based on the degree of their multi-trait associations, FLC, SVP, SOC1, etc. are suggested to have relatively higher pleiotropic potential among others in plant growth, development and flowering process. The approach devised in this study is expected applicable for having basic understanding on any study subject of interest regardless of depth prior knowledge.

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Section: Rootssupporting

confidence: 80%

Functional Overview on Mads Box: A Meta-Review

Adhikari,

Kasahara

2024

Preprint

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“…For example, Arabidopsis AtSPL2/10/11 participate in root regeneration, as the rooting capacity is enhanced in spl10 spl11 double mutants and spl2 spl10 spl11 triple mutants ( Ye et al 2020 ). Likewise, overexpressing SmSPL6 decreased the lateral root number and fresh weight of red sage ( Salvia miltiorrhiza ) while increasing root length ( Cao et al 2021 ), and silencing SPL12 improved root regeneration and nodulation in alfalfa ( M. sativa ) ( Nasrollahi et al 2022 ). Our results revealed that MsSPL13 , an SPL transcription factor gene in the same clade as AtSPL2/10/11 , is a direct target gene of msi-miR156ab and that its heterologous expression inhibited aboveground and root development in transgenic Arabidopsis ( Figs.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA156ab regulates apple plant growth and drought tolerance by targeting transcription factor MsSPL13

Chen

Zhang

et al. 2023

Plant Physiology

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Drought stress substantially reduces the productivity of apple plants and severely restricts the development of the apple industry. Malus sieversii, a wild apple with excellent drought resistance, is a valuable wild resource for rootstock improvement of cultivated apple (Malus domestica). miRNAs and their targets play essential roles in plant growth and stress responses, but their roles in drought stress responses in apple are unknown. Here, we demonstrate that microRNA156ab is upregulated in M. sieversii in response to drought stress. Overexpressing msi-miR156ab promoted auxin accumulation, maintained the growth of apple plants, and increased plant resistance to osmotic stress. Antioxidant enzyme activities and proline contents were also increased in miR156ab-OE transgenic apple lines, which improved drought resistance. The SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor MsSPL13 is the target of msi-miR156ab, as demonstrated by 5-RACE and dual luciferase assays. Heterologous expression of MsSPL13 decreased auxin contents and inhibited growth in Arabidopsis (Arabidopsis thaliana) under normal and stress conditions. The activities of antioxidant enzymes were also suppressed in MsSPL13-OE transgenic Arabidopsis, reducing drought resistance. We showed that MsSPL13 regulates the expression of the auxin-related genes MsYUCCA5, PIN-FORMED7 (MsPIN7), and Gretchen Hagen3-5 (MsGH3-5) by binding to the GTAC cis-elements in their promoters, thereby regulating auxin metabolism. Finally, we demonstrated that the miR156ab-SPL13 module is involved in mediating the difference in auxin metabolism and stress responses between the M. sieversii and M26 (M. domestica) rootstocks. Overall, these findings reveal that the miR156ab-SPL13 module enhances drought stress tolerance in apples by regulating auxin metabolism and antioxidant enzyme activities.

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“…Furthermore, miR156/SPL10 also regulated the root meristem activity and root-derived de novo shoot regeneration via cytokinin responses [ 44 ]. Similarly, in alfalfa, miR156 significantly increased root regenerative capacity and root length by forming a miR156–SPL12–AGL6 genetic module in transgenic lines [ 45 , 46 , 47 ]. A previous finding also showed that high miR156 expression is necessary for adventitious root formation in apple [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

The MdmiR156n Regulates Drought Tolerance and Flavonoid Synthesis in Apple Calli and Arabidopsis

Guo

Wang

Liu

et al. 2023

IJMS

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Drought is the major abiotic stress that limits apple productivity and quality. To date, many important and divergent regulatory functions of miR156/SBP genes in plant growth and development have been well understood. However, little is known about the role of apple miR156 in response to abiotic stress. To better understand the functions of MdmiR156 in abiotic stress tolerance, we constructed the overexpression (OE) and short tandem target mimic (STTM) vector of MdmiR156n and performed its functional analysis through the characterization of transgenic apple calli and Arabidopsis thaliana plants. In this study, MdmiR156n overexpression significantly increased the length of primary roots and the number of lateral roots in transgenic Arabidopsis plants under drought stress. In addition, MdmiR156n transgenic Arabidopsis and apple calli had a lower electrolyte leakage rate and less cell membrane damage than WT and STTM156 after drought stress. Further studies showed that MdmiR156n overexpression promoted the accumulation of flavonoids and scavenging of reactive oxygen species (ROS) under drought conditions in transgenic apple calli and A. thaliana plants. Taken together, overexpression MdmiR156n enhances drought tolerance by regulating flavonoid synthesis and ROS signaling cascades in apple calli and A. thaliana.

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Deciphering the role of SPL12 and AGL6 from a genetic module that functions in nodulation and root regeneration in Medicago sativa

Cited by 6 publications

References 113 publications

Functional Overview on Mads Box: A Meta-Review

Functional Overview on Mads Box: A Meta-Review

MicroRNA156ab regulates apple plant growth and drought tolerance by targeting transcription factor MsSPL13

The MdmiR156n Regulates Drought Tolerance and Flavonoid Synthesis in Apple Calli and Arabidopsis

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