Deciphering the role of human arylamine N-acetyltransferase 1 (NAT1) in breast cancer cell metabolism using a systems biology approach.

Abstract

who constructed the cell lines used as samples in the studies presented in this dissertation, Dr. Carolyn Klinge who I worked with on the Bioenergetics experiments presented in Chapter 3 of this dissertation, Dr. Wolfgang Zacharias, Sabine Waigel, and Vennila Arumugam of the University of Louisville Genomics Core who graciously allowed me to be a part of the library preparation of the RNA-seq (transcriptomics) samples, presented in Chapter 5, Dr. Eric Rouchka and Dr. Xiaohong Li of the KBRIN Bioinformatics Core who did the preprocessing of the transcriptomics data, and Dr. Patrick Trainor who was never too busy to serve as my own personal StackOverflow database. I want to thank all of my collaborators for their kindness, help, and willingness to let me be involved. Furthermore, I would like to thank the Bioinformatics Journal Club Group and Dr. Julia Chariker for providing such a welcoming and supportive environment in which to broaden my bioinformatics knowledge. Thank you to my fellow Hein Lab members, Dr. Raul Salazar-González, Dr. Mariam Habil, and Angeliki Likoudi, for providing a listening ear when times were tough and their friendship. Lastly, I would like to acknowledge and thank all of my friends and family who have provided unwavering encouragement and support in all of my endeavors. I am tremendously grateful for all of you. Several chapters included in this dissertation have been published; Chapter 2 has been published in the International Journal of Oncology and Chapter 3 has been published in Molecular Carcinogenesis; additionally, we intend to publish Chapters 4-7 soon.