Deciphering the response of asymmetry in the hydrophobic chains of novel cationic lipids towards biological function

Abstract

Variations in molecular architecture of the hydrophobic tails of cationic lipids influence cationic liposome*s efficiency of delivering nucleic acids during cell transfection.

Host‐Assisted Delivery of a Model Drug to Genomic DNA: Key Information from Ultrafast Spectroscopy and in silico Study

“…[23b] This corresponds to the higher average amplitude of the donorÀ acceptor distance fluctuations in the latter case than that of the former. The higher amplitude of the donorÀ acceptor distance fluctuations measured in terms of the half-width of the probability distribution curve is consistent with the possibility of system flexibility which manifests conformational relaxation of the model drug [38] at pre-micellar SDS during recognition to DNA. This conformational flexibility of the model drug at pre-micellar concentration of SDS assists in the delivery of TB to DNA, as discussed in the latter section.…”

Barriers and Strategies of Cationic Liposomes for Cancer Gene Therapy

“…The molecular structures of cationic lipids are crucial to cell uptake, endosomal escape, and cytotoxicity. 18 , 146 , 147 , 148 , 149 Kolašinac et al. 95 reported that an inverted conical shape and an aromatic molecule in cationic lipid are indispensable to the fusion induction.…”

“…The molecular structures of cationic lipids are crucial to cell uptake, endosomal escape, and cytotoxicity. 18,[146][147][148][149] Kola sinac et al 95 reported that an inverted conical shape and an aromatic molecule in cationic lipid are indispensable to the fusion induction. Bruininks et al 16 elucidated the molecular effects of lipid tail saturation on lipid fusion and TE and suggested that cationic lipid tail saturation is a necessary condition for these processes.…”

“…2 However, despite its excellent performance in in vitro studies, DOPE is unsuitable for improving gene delivery in vivo, whereas cholesterol-rich CLs work better than their DOPE counterpart to deliver genes in the body with prolonged blood circulation time. 148 Similar to DOPE, cholesterol-rich lipoplexes could partially induce fusiondriven cellular uptake and facilitate endosomal destabilization. 99 Cholesterol has a much smaller hydration repulsion layer than does 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), and thus the substitution of DOPC by cholesterol facilitates the fusion of Lipoplexes enter into cells via endocytosis or membrane fusion.…”

Picosecond-resolved fluorescence resonance energy transfer (FRET) in diffuse reflectance spectroscopy explores biologically relevant hidden molecular contacts in a non-invasive way

“…In the development of chemical and biological experimental techniques Förster resonance energy transfer (FRET) plays a significant role. 1,2 Extreme spatial resolution (1–10 nm) in FRET lies on the nearfield optical communication where the radius of the interaction between two participating molecules (donor and acceptor) is much smaller than the wavelength of light emitted by the donor molecule. In the nearfield region, the emitted photons from the donor molecules, which are duly absorbed by the acceptor, are virtual in nature as their existence violates the conservation of energy and momentum, and hence FRET is known as a radiation-less mechanism.…”