Deciphering the protein translation inhibition and coping mechanism of trichothecene toxin in resistant fungi

Kumari, Indu; Ahmed, Mushtaq; Akhter, Yusuf

doi:10.1016/j.biocel.2016.08.002

Cited by 12 publications

(7 citation statements)

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“…SG was also found to specifically interact with 40S and 60S ribosomal subunit as early as 5 minutes and led to polysome disaggregation in RAW 264.7 cells [10]. It has been suggested that trichothecenes targeted the ribosomal protein L3 (Rpl3) at the peptidyltransferase centre of 60S subunit, which led to protein synthesis inhibition [11,12]. Heterogenous expression of N-terminal fragment of yeast Rpl3 in tobacco conferred resistance to DON [13].…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Mitochondria: a critical target in the toxicity of trichothecenes and potential treatment strategies

Huang¹,

Cui²,

Wu³

et al. 2018

Oncotarget

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Copyright: Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ABSTRACTTrichothecenes are secondary metabolites produced by fungi of genus Fusarium. It was known that trichothecenes can bind to the 60S subunit of the eukaryotic ribosomes, resulting in inhibition of protein synthesis. However, emerging evidences suggest that trichothecenes can target the mitochondria to induce mitochondrial dysfunction and mitochondria-dependent apoptosis. Numerous studies have investigated the mitochondria-associated toxicities induced by trichothecenes. Here, we mainly collected the data associated with mitochondria and its relationship with trichothecene-induced toxic effects. We showed that mitochondria are the main site of reactive oxygen species production and mediate the trichothecene-induced apoptosis in various cells. The mitochondrial membrane permeabilization plays critical roles in this apoptotic process. Trichothecenes can penetrate the mitochondrial membrane due to its amphiphilic property, and inhibit the activities of mitochondrial respiratory chain complexes and electron transfer in mitochondria. Besides, trichothecenes reduce the mitochondrial biogenesis and mitochondrial transcription and translation. Peroxisome proliferator-activated receptor-γ co-activator 1α and mammalian target of rapamycin pathway are closely linked to this process. Trichothecenes can activate various microRNAs that may mediate the protein synthesis inhibition. These microRNAs may have a crosstalk with mitochondria to mediate the mitochondrial dysfunction and transcription repression in mitochondrial genome. Numerous natural compounds have been tested for their effective antioxidant and anti-inflammatorycapacities. Specially, mitochondria-targeted antioxidants and mitophagy exhibit outstanding cytoprotective abilitysince they can reduce the mitochondrial reactive oxygen species and maintain the mitochondrial quality. This review may shed some new light on the mitochondriaassociated mechanism underlying the toxicity of trichothecenes and new preventive ways to combat these mycotoxins. www.impactjournals.com/oncotarget/

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Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Mitochondria: a critical target in the toxicity of trichothecenes and potential treatment strategies

Huang¹,

Cui²,

Wu³

et al. 2018

Oncotarget

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“…These authors suggest that this anxiogenic profile may be modulated by hormonal states (Liang et al, 2008;Viana Borges et al, 2019). This proposal is supported by similar results in female rats by Kumari et al (2016), who suggested that in light of the differences attributable to sex, although greater presence of anxious behaviors are found in females, it is, in fact, males who, when subjected to chronic stressors such as CSI, increase and maintain high levels of these behaviors. Weintraub et al (2010) add that females exhibited higher percentages of entries into the open arms of the EPM (vs. the closed arms) when compared to males; indicating that despite being subjected to the same stressor, the males demonstrated higher anxiety levels than the females.…”

Section: Discussionmentioning

confidence: 69%

“…Social isolation also produces anxiety-type behaviors in rodents, which are demonstrated by their performance in tests such as the Open Field and Elevated-Plus-Maze (EPM; Botelho et al, 2007;Pritchard et al, 2013;Kumari et al, 2016;Shetty and Sadananda, 2017;Sequeira-Cordero et al, 2019). The EPM is one of the most widely used models for the evaluation of anxiety-related behavior (Botelho et al, 2007;Amancio-Belmont et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…It is based upon the natural preference of these animals for dark and protected places, and their anxiety is thus measured by the proportion of test time that they remain in the closed arms of the maze, without coming into contact with the open areas (Pellow et al, 1985;Botelho et al, 2007;Casarrubea et al, 2016). Research indicates that exposure to social isolation in adolescence results in an increased level of locomotor activity, as well as a reduced number of entries into-and time spent in-the open arms of the maze (Kumari et al, 2016;Shetty and Sadananda, 2017;Amancio-Belmont et al, 2020); a pattern which has shown itself to be reversible through the use of various types of anxiolytic drugs such as Diazepam, Alprazolam y Pentylenetetrazol (Pellow et al, 1985;Sorregotti et al, 2013;Sprowles et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Anhedonic Type Behavior and Anxiety Profile of Wistar-UIS Rats Subjected to Chronic Social Isolation

Acero-Castillo

Ardila-Figueroa

Oliveira

2021

Front. Behav. Neurosci.

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Chronic Social Isolation (CSI) is a model of prolonged stress employed in a variety of studies to induce depression and anxious behavior in rats. The present study aims to evaluate the effect of CSI on male Wistar rats in terms of “anhedonic-type” behavior in the Sucrose Preference Test (SPT) and anxiogenic profile in the elevated-plus-maze (EPM) test, as well as evaluating the effect of resocialization upon sucrose consumption. A total of 24 adolescent male Wistar rats were evaluated. The animals were housed either together (communally) or socially isolated for 21 days, and then exposed for four consecutive days to the SPT test [water vs. a 32% sucrose solution (SS)]. Four days later, they were again subjected to the SPT test (32% vs. 0.7% SS), and then tested on the EPM apparatus 3 days later. Following the completion of the anxiogenic profile of the model, the animals were resocialized for 72 h and then re-tested once again using the SPT (32% vs. 0.7% SS). Twenty-four hours after this final consumption, the animals were euthanized to record the weight of their adrenal glands (AG). It was found that exposure to CSI produces anhedonic-type behavior and an anxiogenic profile in adolescent male rats, as evidenced in both the SPT and EPM tests, as well as in the animals’ physiological stress response. It was also demonstrated that resocialization does not reverse the anhedonic-type behavior, nor the physiological response to stress.

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“…In the subsequent animal skin infection experiment, trichodermin could treat rabbit skin mycosis caused by T. mentagrophytes , although a high dosage should be avoided: it could cause rabbit skin cracking at 20 mg/mL, indicating that it could induce skin irritation. Documents have reported that trichodermin metabolites have phytotoxic effects [ 25 ]. The extracellular metabolites and conidia of Trichoderma koningii smf2 were tested by acute oral toxicity test in a mouse model, as well as skin and eye irritation tests in rabbit models.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect and Mechanism of Trichoderma taxi and Its Metabolite on Trichophyton mentagrophyte

Xiao

Liu

et al. 2022

JoF

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Trichophyton mentagrophytes is an important zoonotic dermatophyte, which seriously harms the skin of humans and animals. Chemical drugs are generally used for the prevention and treatment of the disease caused by T. mentagrophytes. Discovering new compounds from natural products is an important approach for new drug development. Trichoderma includes a variety of fungal species used for biological control of phytopathogenic fungi. However, the antifungal effects of Trichoderma and their metabolites on zoonotic fungal pathogens are largely unknown. Here, the effect of trichodermin, a metabolite derived from the plant endophytic fungus Trichoderma taxi, on T. mentagrophytes was examined, and the underlying mechanism was explored. T. mentagrophytes growth was suppressed significantly by trichodermin and completely inhibited under 1000 μg/mL trichodermin. The production and germination of T. mentagrophytes spores were remarkably reduced upon exposure to trichodermin, in comparison with control samples. Treatment of lesions caused by T. mentagrophytes on the rabbit skin with 1 mg/mL trichodermin prompted the healing process significantly; however, 20 mg/mL trichodermin was likely toxic to the skin. Under trichodermin treatment, the number of mitochondria in T. mentagrophytes increased significantly, while a few mitochondria-related genes decreased, indicating possible mitochondrial damage. In transcriptome analysis, the GO terms enriched by DEGs in the trichodermin-treated group included carbohydrate metabolic process, integral component of membrane, intrinsic component of membrane, and carbohydrate binding, while the enriched KEGG pathways comprised biosynthesis of secondary metabolites, glycolysis/gluconeogenesis, and carbon metabolism. By comparing the wild type and a gene deletion strain of T. mentagrophytes, we found that CDR1, an ABC transporter encoding gene, was involved in T. mentagrophytes sensitivity to trichodermin.

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Deciphering the protein translation inhibition and coping mechanism of trichothecene toxin in resistant fungi

Cited by 12 publications

References 31 publications

WITHDRAWN: Mitochondria: a critical target in the toxicity of trichothecenes and potential treatment strategies

WITHDRAWN: Mitochondria: a critical target in the toxicity of trichothecenes and potential treatment strategies

Anhedonic Type Behavior and Anxiety Profile of Wistar-UIS Rats Subjected to Chronic Social Isolation

Inhibitory Effect and Mechanism of Trichoderma taxi and Its Metabolite on Trichophyton mentagrophyte

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