Deciphering the phylogeny of violets based on multiplexed genetic and metabolomic approaches

Chervin, Justine; Talou, Thierry; Audonnet, Marjorie; Dumas, Bernard; Camborde, Laurent; Esquerré-Tugayé, Marie-Thérèse; Roux, Christophe; Cabanac, Guillaume; Marti, Guillaume

doi:10.1016/j.phytochem.2019.04.001

Cited by 14 publications

(8 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…dehnhardtii. More recently, its distinct taxonomic position was refined among a large collection of violets through genotypic and metabolomic analyses ( Chervin et al . 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Phenolic profile of a Parma violet unveiled by chemical and fluorescence imaging

et al. 2021

Self Cite

View full text Add to dashboard Cite

The ability of phenolic compounds to autofluoresce upon illumination by UV or blue light was exploited to explore the nature and distribution of these metabolites within the flower petals, leaves, and roots of the violet, Viola alba subsp. dehnhardtii. This was achieved through a dual complementary approach that combined fluorescence microscopy imaging of living intact tissues and chemical extraction of pulverized material. The blue to red fluorescence displayed by living tissues upon illumination was indicative of their richness in phenolic compounds. Phenolic acids were found in all tissues, while flavonoids characterized the aerial part of the plant, anthocyanidins being restricted to the petals. The chemical quantification of phenolics in plant extracts confirmed their tissue-specific distribution and abundance. A key finding was that the spectral signatures obtained through confocal microscopy of endogenous fluorophores in living tissues and their counterpart extracts share the same fluorescence patterns, pointing out the potential of fluorescence imaging of intact organs for a proper estimation of their phenolic content. In addition, this study highlighted a few distinct morphology cell types, in particular foliar-glandular like structures, and jagged petal cell walls. Alltogether, these data provide a comprehensive histochemical localization of phenolics in living tissues of a violet. Converting fluorescence imaging into a chemical imprint indicated that one can rely on fluorescence microscopy of intact living tissues as a rapid, non-destructive means to follow their phenolic imprint under various environmental conditions.

show abstract

“…dehnhardtii. More recently, its distinct taxonomic position was refined among a large collection of violets through genotypic and metabolomic analyses ( Chervin et al . 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Phenolic profile of a Parma violet unveiled by chemical and fluorescence imaging

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Even if this species remains underutilized, this information is important to enhance Pistacia oil uses. Solid-phase microextraction (SPME) can extract and pre-concentrate VOC from different matrices [28,32]. This method can be used for the characterization of the VOC from P. atlantica oil.…”

Section: Introductionmentioning

confidence: 99%

Pistacia Atlantica Desf., a Source of Healthy Vegetable Oil

et al. 2019

View full text Add to dashboard Cite

Pistacia atlantica, which belongs to the Anacardiaceae family, is an important species for rural people in arid and semi-arid areas. The fruit, rich in oil, is used in traditional medicine for the treatment of various diseases. The oil extracted from this species growing in a northern area of Algeria and its fatty acid composition were previously studied. However, the largest areas where this species is present (traditional cultivation) is located in southern Algeria. Moreover, studies on oil fatty acid composition and essential oil were always conducted separately. This study was performed in order to assess the fatty acid and volatile organic compound composition of P. atlantica vegetable oil. The seeds were collected randomly from Djelfa (300 km South of Algiers, Algeria). Oil content and fatty acid composition were determined by Soxhlet extraction. The seeds contained high concentrations of oil (32–67%). The major fatty acids were oleic (39–49%), linoleic (23.6–31%), and palmitic (21.3–26.6%) acids. The ratio of polyunsaturated fatty acids (PUFA) to saturated fatty acids (SFA) indicated that the content of unsaturated fatty acids was approximately three times higher than that of SFA. This ratio is widely used in epidemiological studies and research on cardiovascular diseases, diabetes, and metabolic syndrome. The ratios of -acids, i.e., -9/-6 and -6/-3, were 1.3–2 and 18.5–38.3, respectively. Crushed seeds were analyzed by headspace solid-phase microextraction (SPME) coupled with gas chromatography–mass spectrometry. More than 40 compounds were identified, mainly monoterpenes (C10H16), such as α-terpinene and terpinolene, but also sesquiterpenes (C15H24) at lower levels. The value of this species as a source of healthy oil rich in -3 acid and its effects on cardiovascular disease risk are discussed.

show abstract

“…The phylogenetic trees constructed with Neighbor‐Joining and Maximum Likelihood methods also obtained similar grouping results ( Figure S1 and Figure S2 ). Combining the phylogenetic analysis and metabolomics determined the phenotypic characteristics of the studied organisms [20] . The present phylogenetic grouping results can be used as a background reference for plant classify cation in metabolomic studies.…”

Section: Resultsmentioning

confidence: 81%

“…Combining the phylogenetic analysis and metabolomics determined the phenotypic characteristics of the studied organisms. [20] The present phylogenetic grouping results can be used as a background reference for plant classify cation in metabolomic studies.…”

Section: Phylogenetic Analysismentioning

confidence: 92%

Metabolite Profiling Based on UPLC‐Q‐TOF‐MS/MS and the Biological Evaluation of Medicinal Plants of Chinese Dichocarpum (Ranunculaceae)

Shen

et al. 2021

Chemistry & Biodiversity

View full text Add to dashboard Cite

The genus Dichocarpum is endemic to East Asia, and many species have been used to treat various diseases. However, phytochemical researches of this genus have been limited to date. In the present study, a metabolomic approach based on UPLC‐Q‐TOF‐MS/MS was used to explore the phytochemical profiles of 10 Chinese Dichocarpum species, and cannabinoid receptor (CB1/CB2) agonistic activities evaluation of these plants was performed. A total of 128 features were putatively annotated, belonging to alkaloids, flavonoids, triterpenes saponins, phenolic acids, and others. Semi‐quantitative statistics demonstrated that alkaloids and flavonoids were widely distributed, with the former the most abundant, whereas triterpenes saponins were mainly distributed in D. fargesii and D. wuchuanense. The phylogenetic results obtained from DNA sequencing assigned the 10 species to three groups. Further results of in silico annotation revealed three chemical families and helped determine the characteristic features of the three groups. In addition, the plant extracts of nine species from this genus showed agonistic activity on CB2 receptors. This comprehensive analysis revealed the chemotype distribution and pharmacophylogenetic relationship, to provide clues for the prospective resource utilization of the medicinal plants from the genus Dichocarpum.

show abstract

Deciphering the phylogeny of violets based on multiplexed genetic and metabolomic approaches

Abstract: Deciphering the phylogeny of violets based on multiplexed genetic and metabolomic approaches. (2019) Phytochemistry, 163. 99-110.

Cited by 14 publications

References 47 publications

Phenolic profile of a Parma violet unveiled by chemical and fluorescence imaging

Phenolic profile of a Parma violet unveiled by chemical and fluorescence imaging

Pistacia Atlantica Desf., a Source of Healthy Vegetable Oil

Metabolite Profiling Based on UPLC‐Q‐TOF‐MS/MS and the Biological Evaluation of Medicinal Plants of Chinese Dichocarpum (Ranunculaceae)

Contact Info

Product

Resources

About